A standardized extract from Paeonia lactiflora and Astragalus membranaceus attenuates liver fibrosis induced by porcine serum in rats

Sun, Wei; Wang, Ling; Líu, Hao; Li, Xiang; Wei, Wei

doi:10.3892/ijmm.2011.844

Cited by 12 publications

(9 citation statements)

References 31 publications

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“…Chinese medical treatment is aimed at adjusting the environmental and human influences through the use of TCM, which exerts a comprehensive effect on the diseases; because the decoction is usually composed of several herbs or minerals according to the symptoms of patients. WYHZTL formula is composed of 10 herbs, among which Radix Astragali membranacei exerts an anti-fibrotic effect in rats induced by porcine serum via down-regulating PDGFR-β, inhibiting hepatic stellate cells proliferation and MAPK activation [ 23 ]. One of the isolated constituents from Herba Glechomae longitubae , asiatic acid, inhibits TGF-β1-induced collagen and PAI-1 expression in keloid fibroblasts through PPAR-γ activation [ 24 ]; Radix Dioscoreae oppositae attenuats CCl (4)-induced hepatic fibrosis in rats in a dose-dependent manner and the attenuation may be related to the antioxidant properties of Radix Dioscoreae oppositae [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis

Han¹,

Bian²,

Ouyang³

et al. 2016

BMC Complement Altern Med

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BackgroundFibrosis is a major contributor to systemic sclerosis (SSc)-related morbidity, and rapid, progressive skin involvement predicts later mortality. Western medicine therapies for SSc cannot produce satisfactory effects currently, while Traditional Chinese Medicine (TCM), such as the Wenyang Huazhuo Tongluo (WYHZTL) formula, a Chinese herbal decoction, has shown amazing anti-fibrosis efficacy on SSc in clinical applications. This study is aiming to investigate the anti-fibrotic mechanism of WYHZTL formula for the treatment of SSc.MethodsFibroblasts from primary culture of skin lesions of SSc patients were exposed to rat medicated sera containing WYHZTL or XAV939, a small-molecule inhibitor of both tankyrase 1/2 and Wnt/β-catenin pathway. Cell counting kit-8 assay and Annexin V FITC/PI apoptosis kit were used to analyze cell proliferation and apoptosis in fibroblasts, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were used to detect the mRNA and protein levels of cyclin D1 and survivin.ResultsAfter 28, 48 and 72 h of incubation, the proliferative ability of the fibroblasts cells was obviously reduced by the sera containing WYHZTL compared with that in the control group; the percentage of apoptotic cell population in the sera containing WYHZTL treated fibroblasts cells was significantly higher than that in those treated with the control sera, and was about similar to that in those treated with XAV939. The sera containing WYHZTL could down-regulate both mRNA and protein levels of cyclin D1 and survivin, compared with the control group.ConclusionsThe present study demonstrates the antiproliferative and pro-apoptotic actions of WYHZTL formula against fibroblasts and the effect may be related to the down-regulation of mRNA and protein levels of cyclin D1 and survivin in SSc.

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Section: Discussionmentioning

confidence: 99%

Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis

Han¹,

Bian²,

Ouyang³

et al. 2016

BMC Complement Altern Med

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“…Astragalus membranaceus (Fisch) Bunge, known as Huang-qi in Chinese or Radix Astragali in Latin, is one of the most popular herbal medicines worldwide, and it has been widely used as a Qi-tonifying medicine in the People’s Republic of China, Mongolia, and Korea for a long time. 1 , 2 Pharmacological studies have shown that A. membranaceus exhibits many beneficial effects, including immunomodulation, 3 – 5 antihyperglycemic effects and improved insulin sensitivity, 6 – 8 anti-inflammation effects, 9 , 10 antioxidant effects, 11 , 12 antiviral effects, 13 , 14 hepatoprotection effects, 15 , 16 antineoplastic effects, protection of cardiovascular function, 17 and so on. Meanwhile, phytochemical studies have displayed >100 compounds from A. membranaceus (root), such as flavonoids, polysaccharides, saponins, sucroses, amino acids, and phenolic acids.…”

Section: Introductionmentioning

confidence: 99%

Formononetin, an active compound of <em>Astragalus membranaceus</em> (Fisch) Bunge, inhibits hypoxia-induced retinal neovascularization via the HIF-1α/VEGF signaling pathway

Wu¹,

Ke²,

Ma³

et al. 2016

DDDT

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BackgroundIt has been reported that formononetin (FMN), one of the main ingredients from famous traditional Chinese medicine “Huang-qi” (Astragalus membranaceus [Fisch] Bunge) for Qi-tonifying, exhibits the effects of immunomodulation and tumor growth inhibition via antiangiogenesis. Furthermore, A. membranaceus may alleviate the retinal neovascularization (NV) of diabetic retinopathy. However, the information of FMN on retinal NV is limited so far. In the present study, we investigated the effects of FMN on the hypoxia-induced retinal NV and the possible related mechanisms.Materials and methodsThe VEGF secretion model of acute retinal pigment epithelial-19 (ARPE-19) cells under chemical hypoxia was established by the exposure of cells to 150 μM CoCl2 and then cells were treated with 3-(5′-hydroxymethyl-2′-furyl)-1-benzylindazole (YC-1, a potent HIF-1α inhibitor, 1.0 μg/mL) or different concentrations of FMN (0.2 μg/mL, 1.0 μg/mL, and 5.0 μg/mL). The supernatants of cells were collected 48 hours later to measure the VEGF concentrations, following the manufacturer’s instruction. The mRNA expressions of VEGF, HIF-1α, PHD-2, and β-actin were analyzed by quantitative reverse transcription polymerase chain reaction, and the protein expressions of HIF-1α and PHD-2 were determined by Western blot analysis. Furthermore, the rats with retinopathy were treated by intraperitoneal administration of conbercept injection (1.0 mg/kg) or FMN (5.0 mg/kg and 10.0 mg/kg) in an 80% oxygen atmosphere. The retinal avascular areas were assessed through visualization of the retinal vasculature by adenosine diphosphatase staining and hematoxylin and eosin staining.ResultsFMN can indeed inhibit the VEGF secretion of ARPE-19 cells under hypoxia, downregulate the mRNA expression of VEGFA and PHD-2, and decrease the protein expression of VEGF, HIF-1α, and PHD-2 in vitro. Furthermore, FMN can prevent hypoxia-induced retinal NV in vivo.ConclusionFMN can ameliorate retinal NV via the HIF-1α/VEGF signaling pathway, and it may become a potential drug for the prevention and treatment of diabetic retinopathy.

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“…Liver fibrosis which is a common feature of the majority of chronic liver diseases is considered as an important medical problem due to its high morbidity and mortality [ 1 , 2 ]. Hepatitis viral infections are the most common cause, and drug abuse, autoimmune disorders, biliary obstruction and metabolic disorders due to mineral overload are also contributed to the disease [ 3 , 4 ], which is characterized as excessive accumulation of collagen and extracellular matrix proteins within the perisinusoidal space [ 5 , 6 ]. Advanced liver fibrosis could leads to cirrhosis and liver failure eventually.…”

Section: Introductionmentioning

confidence: 99%

“…Advanced liver fibrosis could leads to cirrhosis and liver failure eventually. Now there is few effective medical treatments approaches for liver fibrosis [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Protective effects of extracts from Pomegranate peels and seeds on liver fibrosis induced by carbon tetrachloride in rats

Wei

Fang

Yang

et al. 2015

BMC Complement Altern Med

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BackgroundLiver fibrosis is a feature in the majority of chronic liver diseases and oxidative stress is considered to be its main pathogenic mechanism. Antioxidants including vitamin E, are effective in preventing liver fibrogenesis. Several plant-drived antioxidants, such as silymarin, baicalin, beicalein, quercetin, apigenin, were shown to interfere with liver fibrogenesis. The antioxidans above are polyphenols, flavonoids or structurally related compounds which are the main chemical components of Pomegranate peels and seeds, and the antioxidant activity of Pomegranate peels and seeds have been verified. Here we investigated whether the extracts of pomegranate peels (EPP) and seeds (EPS) have preventive efficacy on liver fibrosis induced by carbon tetrachloride (CCl4) in rats and explored its possible mechanisms.MethodsThe animal model was established by injection with 50 % CCl4 subcutaneously in male wistar rats twice a week for four weeks. Meanwhile, EPP and EPS were administered orally every day for 4 weeks, respectively. The protective effects of EPP and EPS on biochemical metabolic parameters, liver function, oxidative markers, activities of antioxidant enzymes and liver fibrosis were determined in CCl4-induced liver toxicity in rats.ResultsCompared with the sham group, the liver function was worse in CCl4 group, manifested as increased levels of serum alanine aminotransferase, aspartate aminotransferase and total bilirubin. EPP and EPS treatment significantly ameliorated these effects of CCl4. EPP and EPS attenuated CCl4–induced increase in the levels of TGF-β1, hydroxyproline, hyaluronic acid laminin and procollagen type III. They also restored the decreased superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and inhibited the formation of lipid peroxidized products in rats treated with CCl4.ConclusionThe EPP and EPS have protective effects against liver fibrosis induced by CCl4, and its mechanisms might be associated with their antioxidant activity, the ability of decreasing the level of TGF-β1 and inhibition of collagen synthesis.

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A standardized extract from Paeonia lactiflora and Astragalus membranaceus attenuates liver fibrosis induced by porcine serum in rats

Cited by 12 publications

References 31 publications

Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis

Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis

Formononetin, an active compound of <em>Astragalus membranaceus</em> (Fisch) Bunge, inhibits hypoxia-induced retinal neovascularization via the HIF-1α/VEGF signaling pathway

Protective effects of extracts from Pomegranate peels and seeds on liver fibrosis induced by carbon tetrachloride in rats

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A standardized extract from Paeonia lactiflora and Astragalus membranaceus attenuates liver fibrosis induced by porcine serum in rats

Cited by 12 publications

References 31 publications

Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis

Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis

Formononetin, an active compound of <em>Astragalus membranaceus</em> (Fisch) Bunge, inhibits hypoxia-induced retinal neovascularization via the HIF-1&alpha;/VEGF signaling pathway

Protective effects of extracts from Pomegranate peels and seeds on liver fibrosis induced by carbon tetrachloride in rats

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Formononetin, an active compound of <em>Astragalus membranaceus</em> (Fisch) Bunge, inhibits hypoxia-induced retinal neovascularization via the HIF-1α/VEGF signaling pathway