A Status Update on Pharmaceutical Analytical Methods of Aminoglycoside Antibiotic: Amikacin

Raut, Adishri; Sharma, Dhvani; Suvarna, Vasanti

doi:10.1080/10408347.2020.1803042

Cited by 17 publications

(11 citation statements)

References 86 publications

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“…When an antibiotic is administered, some of the antibiotic is excreted and can cause environmental damage, while the rest remains in the body. Because AGs are highly polar, they are poorly preserved in conventional reversed-phase LC, and they cannot be detected using ultraviolet or fluorescence detectors unless pre-or post-column derivatization is performed because they do not contain chromophores or fluorophores [42][43][44][45]. However, derivatization makes the instrument conditions more complex, and the additional processing steps required lead to loss of the analytical substance.…”

Section: Introductionmentioning

confidence: 99%

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Luan

Wang

et al. 2020

Antibiotics

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Antibiotic abuse is becoming increasingly serious and the potential for harm to human health and the environment has aroused widespread social concern. Aminoglycoside antibiotics (AGs) are broad-spectrum antibiotics that have been widely used in clinical and animal medicine. Consequently, their residues are commonly found in animal-derived food items and the environment. A simple, rapid, and sensitive detection method for on-site screening and detection of AGs is urgently required. In recent years, with the development of molecular detection technology, nucleic acid aptamers have been successfully used as recognition molecules for the identification and detection of AGs in food and the environment. These aptamers have high affinities, selectivities, and specificities, are inexpensive, and can be produced with small batch-to-batch differences. This paper reviews the applications of aptamers for AG detection in colorimetric, fluorescent, chemiluminescent, surface plasmon resonance, and electrochemical sensors for the analysis in food and environmental samples. This study provides useful references for future research.

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Section: Introductionmentioning

confidence: 99%

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Luan

Wang

et al. 2020

Antibiotics

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“…Due to the increased clinical significance of amikacin, several analytical methods have been reported in the published literature for its estimation in pharmaceutical dosage forms [ 7–11 ] and in biological fluids. [ 12–18 ] Analytical techniques such as spectrophotometry, [ 19,20 ] spectrofluorimetry, [ 21–24 ] voltammetry, [ 25 ] chemiluminescence, [ 26,27 ] ultra‐performance liquid chromatography–tandem mass spectrometry, [ 28 ] high performance liquid chromatography (HPLC), [ 19–23 ] and capillary electrophoresis [ 18,29,30 ] have been reported for amikacin quantification.…”

Section: Introductionmentioning

confidence: 99%

“…estimation in pharmaceutical dosage forms [7][8][9][10][11] and in biological fluids. [12][13][14][15][16][17][18] Analytical techniques such as spectrophotometry, [19,20] spectrofluorimetry, [21][22][23][24] voltammetry, [25] chemiluminescence, [26,27] ultra-performance liquid chromatography-tandem mass spectrometry, [28] high performance liquid chromatography (HPLC), [19][20][21][22][23] and capillary electrophoresis [18,29,30] have been reported for amikacin quantification.…”

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confidence: 99%

“…For these reasons, spectrophotometric methods are most widely used technique in pharmaceutical analysis due to their inherent simplicity and wide availability in most QC laboratories. [7,31] Amikacin sulfate is a nonfluorescent compound and has intrinsic structural inadequacies due to the absence of a chromophore or conjugate system that can produce reliable ultraviolet (UV) light/ fluorescence signals. Direct estimation or quantification of amikacin is therefore difficult.…”

mentioning

confidence: 99%

“…Direct estimation or quantification of amikacin is therefore difficult. [7,32] However, the drug contains four primary amine functional groups (Figure 1) that can be derivatized chemically to generate reliable UV/fluorescence signals, which can, in turn, be used as a basis for the quantification of amikacin. [33][34][35][36][37][38] During the last few years, significant research has driven the field of nanomedicine, especially in the delivery of drugs.…”

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A novel high throughput 96‐well‐based fluorimetric method to measure amikacin in pharmaceutical formulations: development using response surface methodology

et al. 2022

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An aminoglycoside antibiotic, amikacin, is used to treat severe and recurring bacterial infections. Due to the absence of a chromophore, however, amikacin must be extensively derivatized before being quantified, both in analytical and bioanalytical samples. In this study, for the first time, we developed a simple and sensitive method for measuring amikacin sulfate using spectrofluorimetry with a 96-well plate reader, based on the design of the experiment's approach. To develop a robust and reproducible spectrofluorimetric method, the influence of essential attributes, namely pH of the buffer, heating temperature, and concentration of reagents, were evaluated using univariate analysis followed by multivariate analysis (central composite design).International Conference of Harmonization guidelines were used to validate the optimized method. The developed technique is linear from 1.9 to 10 μg/ml with a regression coefficient of 0.9991. The detection and quantification limits were 0.649 μg/ml and 1.9 μg/ml, respectively. For the developed method, both intraday and interday precision (%RSD) were less than 5%. Using the method, amikacin concentrations were quantified in prepared amikacin liposomes and commercial formulations of Amicin ® . The developed method greatly reduces sample volume and is a rapid, high throughput microplate-based fluorescence approach for the convenient and costeffective measurement of amikacin in pharmaceutical formulations. In comparison with previously published approaches, the suggested method allowed for quick analysis of a high number of samples in a short amount of time (96 samples in 125 sec), resulting in an average duration of analysis of 1.3 sec per sample.

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Comprehensive discovery and migration evaluation of antimicrobial drugs and their transformation products in a swine farm by target, suspect, and nontarget screening

Huang,

Hu,

Yang

et al. 2023

Environment International

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A Status Update on Pharmaceutical Analytical Methods of Aminoglycoside Antibiotic: Amikacin

Cited by 17 publications

References 86 publications

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

A novel high throughput 96‐well‐based fluorimetric method to measure amikacin in pharmaceutical formulations: development using response surface methodology

Comprehensive discovery and migration evaluation of antimicrobial drugs and their transformation products in a swine farm by target, suspect, and nontarget screening

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