A stereological study on calculation of volume values regarding lumbosacral segments of quails

Abstract

In the study, total segment, grey matter and white matter volume values of lumbosacral segment of spinal cord in quails (Coturnix coturnix japonica) were stereologically examined. Regardless of sex, six adult quails were used as material. After the materials were perfused, they were dissected and their spinal cords were uncovered. Tissue samples were taken from each lumbosacral segment of spinal cord. After the tissue fixation process, 6–7 sections having a 5‐µm thickness were separated in every 50th section as from the first section, where the tissue was seen, among tissue samples of each segment. These segments were stained with haematoxylin–eosin staining. They were photographed via a microscope. By using the Cavalieri's principle, the volume values of both the total segment, grey matter (GM) and white matter (WM) in each lumbosacral segment were separately calculated. All these calculations were performed by using SHTEREOM 1.5 program. As a result, total volume, grey matter and white matter volume values of lumbosacral segments and white matter/total volume, grey matter/total volume, and grey matter/white matter volume fractions were obtained.

CT anatomy of the head in the Ile de France sheep

“…(2018) have conducted a developmental study on the spinal cord of sheep embryo using stereological methods. More recently, the volumetric properties of the lumbo‐sacral spinal cord in quail and thoracic spinal cord in ducks were investigated (Cakmak & Ragbetli, 2019; Cakmak & Karadag, 2019). Because the Cavalieri method was older and the CT scan software was more reliable, we decided to use the automatic method for volume calculations so as not to commit potential human errors in measurement and to increase accuracy.…”

Applied anatomy of the skull in the Arabian horse: A computed tomographic, cross‐sectional, volumetric and morphometric study

“…The combination of cross sections and stereological procedures has less frequently used for volume estimation of organs in veterinary medicine. Only a few studies were conducted on the spinal cord of sheep embryo (Sadeghinezhad et al., 2018 ), quail and ducks (Cakmak & Karadag, 2019 ; Cakmak & Ragbetli, 2019 ).…”