A stereotaxic apparatus for the study of the central nervous structures in the pig

Marcilloux, J.C.; Rampin, Olivier; Felix, Marie-Bernadette; Laplace, J.P.; Albé-Fessard, D

doi:10.1016/0361-9230(89)90118-4

Cited by 21 publications

(21 citation statements)

References 7 publications

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“…Depending on the tools used, two stereotaxic systems have been proposed. Using ventriculography, the horizontal zero plane was defined on the basis of the plane connecting the recessus preopticus to the posterior commissure (CP) and CP as the origin of the system (Marcilloux et al, 1989;Felix et al, 1999). The recessus preopticus was found to correlate closely with the anterior commissure defining a system similar to that commonly used in human and primate (Felix et al, 1999).…”

Section: Brain Imaging and Neurosurgery In Pigmentioning

confidence: 99%

“…Stereotaxic and image guided surgery Several stereotaxic atlases of the pig brain have been described (Yoshikawa, 1968;Szteyn et al, 1980;SalinasZeballos et al, 1986;Felix et al, 1999;Watanabe et al, 2001) as well as specific stereotaxic instruments either for piglets (Salinas-Zeballos et al, 1986), pigs weighing between 30 and 40 kg (Poceta et al, 1981;Saito et al, 1998) or adults (Marcilloux et al, 1989;van Eerdenburg and Dierx, 2002). The stereotaxic frame devoted to the pig developed by Marcilloux et al (1989) was based on the fixation of the skull by the infraorbital ridges, the hard palate and the auditory canals.…”

Section: Brain Imaging and Neurosurgery In Pigmentioning

confidence: 99%

“…Six Large White 3 Landrace female pigs aged of 3 months and weighing 30 kg were used in this study. Neuronavigation was based on images acquired with a CT scanner while pigs were maintained in a custom-made stereotaxic frame similar to that described by Marcilloux et al (1989). The CT images were transferred to a DICOM PACS Workstation running OsiriX, an Open Source Dicom viewer (Rosset et al, 2004) allowing 3D reconstruction, and direct length and angle measurements (Figure 6).…”

Section: Examples Of Studies On the Porcine Central Nervous Systemmentioning

confidence: 99%

“…The stereotaxic frame devoted to the pig developed by Marcilloux et al (1989) was based on the fixation of the skull by the infraorbital ridges, the hard palate and the auditory canals. The ear-bars were carried by a swivelling system allowing adaptation to the orientation of the auditory canals, perpendicular to the sagittal plane in the new-born piglet but oblique in the weaned pig (Poceta et al, 1981;Salinas-Zeballos et al, 1986;Marcilloux et al, 1989). Unfortunately, this system may have some adverse effects on the eardrums and cannot be used with Gö ttingen mini-pigs.…”

mentioning

confidence: 99%

“…Saito et al (1998) have reported the reliability of this method and minimal variation in distances between some external bony landmarks. Despite the fact that this strategy might be successful in young pig, there is a too large inter-individual variation in skull morphology in growing animals and these landmarks may vary considerably as the age of the animal increases (Marcilloux et al, 1989;Bjarkam et al, 2004). In addition, measures based on bone structures can hardly be applied generally since pig present considerable inter-individual and inter-breed skull variations in size and shape.…”

mentioning

confidence: 99%

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The pig model in brain imaging and neurosurgery

Sauleau

Lapouble

Val‐Laillet

et al. 2009

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The pig model is increasingly used in the field of neuroscience because of the similarities of its brain with human. This review presents the peculiarities of the anatomy and functions of the pig brain with specific reference to its human counterpart. We propose an approximate mapping of the pig's cortical areas since a comprehensive description of the equivalent of Brodmann's areas is lacking. On the contrary, deep brain structures are received more consideration but a true three-dimensional (3D) atlas is still eagerly required. In the second section, we present an overview of former works describing the use of functional imaging and neuronavigation in the pig model. Recently, the pig has been increasingly used for molecular imaging studies using positron emission tomography (PET). Indeed, the large size of its brain is compatible with the limited spatial resolution of the PET scanner built to accommodate a human being. Similarly, neuronavigation is an absolute requirement to target deep brain areas in human and in pig since the surgeon cannot rely on external skull structures for zeroing the 3D reference frame. Therefore, a large body of methodological refinements has been dedicated to image guided surgery in the pig model. These refinements allow now a millimetre precision: an absolute requirement for basal nuclei targeting. In the third section, several examples of ongoing studies in our laboratory were presented to illustrate the intricacies of using the pig model. For both examples, after a brief description of the scientific context of the experiment, we present, in detail, the methodological steps required to achieve the experimental goals, which are specific to the porcine model. Finally, in the fourth section, the anatomical variations depending on the breed and age are discussed in relation with neuronavigation and brain surgery. The need for a digitized multimodality brain atlas is also highlighted.Keywords: pig, neuroimaging, single photon emission tomography, neuronavigation, deep brain stimulation ImplicationsThe pig model is increasingly used in the field of neuroscience because of the similarities of its brain with human. Indeed, aside from the rodent model there is a critical need for a large animal model ethically acceptable, i.e. excluding non-human primates. In this review we present some experiments dealing with the various procedures achievable in the pig model ranging from image-guided brain surgery to functional brain imaging studies. The recent advances in functional imaging data processing pointed out our partial knowledge of pig brain neuro-anatomy and the requirement for a digitalized atlas matching MRI (magnetic resonance imaging) and histological resources. IntroductionSwine have been used extensively as a model of human in biomedical researches such as cardiovascular, metabolic and transplantation (Phillips et al., 1982;Larsen and Rolin, 2004;Imai et al., 2006;Groth, 2007). In the last decade, an increasing number of studies in the field of neuroscience has been reported (Lind et ...

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Section: Brain Imaging and Neurosurgery In Pigmentioning

confidence: 99%