A straightforward LC-MS/MS analysis to study serum profile of short and medium chain fatty acids

Dei, Michele; Paroni, Rita; Saccardo, Anna; Casagni, Eleonora; Arnoldi, Sebastiano; Gambaro, Veniero; Saresella, Marina; Clerici, Mario; Rosa, Francesca La; Marventano, Ivana; Piancone, Federica; Roda, Gabriella

doi:10.1016/j.jchromb.2020.121982

Cited by 40 publications

(28 citation statements)

References 24 publications

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“…Excellent chromatographic resolution and characteristic fragments of DMED/DEEA-SCFAs derivatives were also simultaneously J o u r n a l P r e -p r o o f achieved. These enhanced signals further improved the assay validity of the method with lower LOD, LOQs and a better linear range as previously described (21)(22)(23)(24).…”

Section: Methods Validationsupporting

confidence: 66%

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Zhang

Yang

Wang

et al. 2021

Journal of Lipid Research

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Methods Validationsupporting

confidence: 66%

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Zhang

Yang

Wang

et al. 2021

Journal of Lipid Research

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“…Butyric and caproic acids were extracted and analyzed by LC-MS/MS according to the protocol described in Dei Cas ( 39 ). Briefly, serum (50 μl) was deproteinized by isopropanol (100 μl) and fatty acids derivatized with nitrophenylhydrazine.…”

Section: Methodsmentioning

confidence: 99%

Alterations in Circulating Fatty Acid Are Associated With Gut Microbiota Dysbiosis and Inflammation in Multiple Sclerosis

et al. 2020

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Background: Butyric acid (BA) is a short-chain fatty acid (SCFA) with anti-inflammatory properties, which promotes intestinal barrier function. Medium-chain fatty acids (MCFA), including caproic acid (CA), promote TH1 and TH17 differentiation, thus supporting inflammation. Aim: Since most SCFAs are absorbed in the cecum and colon, the measurement of BA in peripheral blood could provide information on the health status of the intestinal ecosystem. Additionally, given the different immunomodulatory properties of BA and CA the evaluation of their serum concentration, as well as their ratio could be as a simple and rapid biomarker of disease activity and/or treatment efficacy in MS. Methods: We evaluated serum BA and CA concentrations, immune parameters, intestinal barrier integrity and the gut microbiota composition in patients with multiple sclerosis (MS) comparing result to those obtained in healthy controls. Results: In MS, the concentration of BA was reduced and that of CA was increased. Concurrently, the microbiota was depleted of BA producers while it was enriched in mucin-degrading, pro-inflammatory components. The reduced serum concentration of BA seen in MS patients correlated with alterations of the barrier permeability, as evidenced by the higher plasma concentrations of lipopolysaccharide and intestinal fatty acid-binding protein, and inflammation. Specifically, CA was positively associated with CD4+/IFNγ+ T lymphocytes, and the BA/CA ratio correlated positively with CD4+/CD25 high /Foxp3+ and negatively with CD4+/IFNγ+ T lymphocytes. Conclusion: The gut microbiota dysbiosis found in MS is possibly associated with alterations of the SCFA/MCFA ratio and of the intestinal barrier; this could explain the chronic inflammation that characterizes this disease. SCFA and MCFA quantification could be a simple biomarker to evaluate the efficacy of therapeutic and rehabilitation procedures in MS.

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“…In addition, most studies measure biomarkers in plasma or serum, while red blood cells are usually not considered. Thus, this blood fraction would not compete for sample volume allotment with other measurements where the use of plasma or serum is mandatory, such as the soluble immune system proteins [ 24 ], coagulation factors, circulating hormones, and neuro-endocrine parameters [ 25 ] and the plasma metabolome [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Griess Assay of Nitrite and Nitrate in Plasma and Red Blood Cells for Human Physiology Studies under Extreme Conditions

Brizzolari

Dei

Cialoni³

et al. 2021

Molecules

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The metabolism of nitric oxide plays an increasingly interesting role in the physiological response of the human body to extreme environmental conditions, such as underwater, in an extremely cold climate, and at low oxygen concentrations. Field studies need the development of analytical methods to measure nitrite and nitrate in plasma and red blood cells with high requirements of accuracy, precision, and sensitivity. An optimized spectrophotometric Griess method for nitrite–nitrate affords sensitivity in the low millimolar range and precision within ±2 μM for both nitrite and nitrate, requiring 100 μL of scarcely available plasma sample or less than 50 μL of red blood cells. A scheduled time-efficient procedure affords measurement of as many as 80 blood samples, with combined nitrite and nitrate measurement in plasma and red blood cells. Performance and usefulness were tested in pilot studies that use blood fractions deriving from subjects who dwelt in an Antarctica scientific station and on breath-holding and scuba divers who performed training at sea and in a land-based deep pool facility. The method demonstrated adequate to measure low basal concentrations of nitrite and high production of nitrate as a consequence of water column pressure-triggered vasodilatation in deep-water divers.

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A straightforward LC-MS/MS analysis to study serum profile of short and medium chain fatty acids

Cited by 40 publications

References 24 publications

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Alterations in Circulating Fatty Acid Are Associated With Gut Microbiota Dysbiosis and Inflammation in Multiple Sclerosis

High-Throughput Griess Assay of Nitrite and Nitrate in Plasma and Red Blood Cells for Human Physiology Studies under Extreme Conditions

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