A strategic crossflow filtration methodology for the initial purification of promegapoietin from inclusion bodies

Ledung, Erika; Eriksson, Per-Olov; Oscarsson, Sven

doi:10.1016/j.jbiotec.2009.02.016

Cited by 11 publications

(5 citation statements)

References 30 publications

(38 reference statements)

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“…Ultrafiltration is used for purification and protein concentration (Ledung et al, 2009;Emami et al, 2019;Palombarini et al, 2019). Diafiltration via an ultrafiltration membrane is used for desalting recombinant proteins (Kovács, 2016).…”

Section: Membrane Filtration-based Techniquesmentioning

confidence: 99%

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi

Shrivastava

2019

Front. Bioeng. Biotechnol.

400

243

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Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind. Recent progress in the area of recombinant DNA technologies has paved the way to producing recombinant proteins that can be used as therapeutics, vaccines, and diagnostic reagents. Recombinant proteins for these applications are mainly produced using prokaryotic and eukaryotic expression host systems such as mammalian cells, bacteria, yeast, insect cells, and transgenic plants at laboratory scale as well as in large-scale settings. The development of efficient bioprocessing strategies is crucial for industrial production of recombinant proteins of therapeutic and prophylactic importance. Recently, advances have been made in the various areas of bioprocessing and are being utilized to develop effective processes for producing recombinant proteins. These include the use of high-throughput devices for effective bioprocess optimization and of disposable systems, continuous upstream processing, continuous chromatography, integrated continuous bioprocessing, Quality by Design, and process analytical technologies to achieve quality product with higher yield. This review summarizes recent developments in the bioprocessing of recombinant proteins, including in various expression systems, bioprocess development, and the upstream and downstream processing of recombinant proteins.

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Section: Membrane Filtration-based Techniquesmentioning

confidence: 99%

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi

Shrivastava

2019

Front. Bioeng. Biotechnol.

400

243

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“…Since these inclusion bodies are biologically inactive, therefore, in vitro refolding of these proteins are required to make it biologically active (Fahnert et al, 2004; Singh and Panda, 2005; Ledung et al, 2009; Singh et al, 2015). …”

Section: Purification Process Developmentmentioning

confidence: 99%

Sophisticated Cloning, Fermentation, and Purification Technologies for an Enhanced Therapeutic Protein Production: A Review

Gupta

Shukla

2017

Front. Pharmacol.

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The protein productions strategies are crucial towards the development of application based research and elucidating the novel purification strategies for industrial production. Currently, there are few innovative avenues are studies for cloning, upstream, and purification through efficient bioprocess development. Such strategies are beneficial for industries as well as proven to be vital for effectual therapeutic protein development. Though, these techniques are well documented, but, there is scope of addition to current knowledge with novel and new approaches and it will pave new avenues in production of recombinant microbial and non-microbial proteins including secondary metabolites. In this review, we have focussed on the recent development in clone selection, various modern fermentation and purification technologies and future directions in these emerging areas. Moreover, we have also highlighted notable perspectives and challenges involved in the bioengineering of such proteins, including quality by design, gene editing and pioneering ideas. The biopharmaceutical industries continue to shift towards more flexible, automated platforms and economical product development, which in turn can help in developing the cost effective processes and affordable drug development for a large community.

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“…The clearance of DNA, hcPs, and endotoxins at the different stages of recombinant protein purification was evaluated during downstream process optimization for monoclonal antibody [25], promegapoietin [26], human granulocyte colony-stimulating factor (rhg-cSF) [27], and interferon γ (INF-γ) [28] expression in E. coli. Although expressed in the same host cell, the impurity levels present enormous variation among the different proteins and processes (Table 13.9).…”

Section: Endotoxinmentioning

confidence: 99%

Biotechnological Products

Bohrer

2012

Sources of Contamination in Medicinal Products and Medical Devices

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A strategic crossflow filtration methodology for the initial purification of promegapoietin from inclusion bodies

Cited by 11 publications

References 30 publications

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Sophisticated Cloning, Fermentation, and Purification Technologies for an Enhanced Therapeutic Protein Production: A Review

Biotechnological Products

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