A Strategy for assessing peak purity of pharmaceutical peptides in reversed-phase chromatography methods using two-dimensional liquid chromatography coupled to mass spectrometry. Part II: Development of second-dimension gradient conditions

Stoll, Dwight R.; Sylvester, Maria; Euerby, Melvin R.; Buckenmaier, Stephan; Petersson, Patrik

doi:10.1016/j.chroma.2023.463873

Cited by 7 publications

(5 citation statements)

References 13 publications

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“…The separation of isomers is emphasised, since these compounds with identical m/z are not readily differentiated by MS. The study is divided into two parts: part I is related to selection of columns and mobile phases [current publication] whereas part II is related to the definition of the 2 D gradient elution conditions, and additional proof-of-concept 2D-LC-MS separations that illustrate the power of the strategy [15].…”

Section: Discussionmentioning

confidence: 99%

“…An Agilent 1290 Infinity II 2D-LC system was used for the collection of the data shown in Figure 8. A generalised illustration of this system for multiple heart-cutting is provided as supplemental information in Part II ( [15] Figure S1). The first dimension consisted of an autosampler, a binary pump with a 380 µL Jet Weaver mixer, a thermostated column compartment, and a UV absorbance detector with a 6 mm semi-micro flow cell.…”

Section: Methodsmentioning

confidence: 99%

“…The study is divided into two papers; Part I is related to the selection of columns and mobile phases [current publication], whereas Part II is related to the definition of the second dimension chromatographic gradient conditions and the demonstration of 2D-LC-MS methods developed using this approach [15].…”

Section: Introductionmentioning

confidence: 99%

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A strategy for assessing peak purity of pharmaceutical peptides in reversed-phase chromatography methods using two-dimensional liquid chromatography coupled to mass spectrometry. Part I: Selection of columns and mobile phases

Petersson

Buckenmaier

Euerby

et al. 2023

Journal of Chromatography A

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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A strategy for assessing peak purity of pharmaceutical peptides in reversed-phase chromatography methods using two-dimensional liquid chromatography coupled to mass spectrometry. Part I: Selection of columns and mobile phases

Petersson

Buckenmaier

Euerby

et al. 2023

Journal of Chromatography A

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“…Petersson et al published a study focused on defining the second-dimension chromatographic gradient conditions and demonstrating the 2D-LC-MS methods developed using this approach [62].…”

Section: Two-dimensional Separation Methodsmentioning

confidence: 99%

Advances in Therapeutic Peptides Separation and Purification

Al Musaimi,

Jaradat

2024

Separations

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Peptides are gaining prominence in various fields, including the pharmaceutical industry. To meet regulatory requirements, they must achieve a certain purity threshold to ensure safe administration. Numerous purification technologies have been employed to purify peptides, aiming to reduce cost and time while being sustainable and efficient. These include chromatography, magnetic nanoparticles, isoelectric focusing, and membrane filtration. The physicochemical properties of peptides are the main driving element behind these technologies. While chromatographic separation remains the gold standard for peptide separation and purification, with various models to predict the elution behaviors of peptides, other technologies have demonstrated their capability to meet the performance of established chromatographic methodologies, with better productivity and reduced cost. This opens the door for further investigational studies to assess these outcomes and potentially introduce new techniques for peptide purification. In this review, we examine these technologies in terms of their efficiency and their ability to meet sustainability requirements, concluding with remarks and an outlook on future advancements.

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“…Both, structure and function of proteins are often investigated by means of their enzymatically generated peptide fragments in the so called bottom-up or shotgun proteomics [10][11][12][13]. These peptide fragments are separated and identified by liquid chromatography (LC), 2-DE, and capillary electrophoresis (CE) methods online coupled with MS detection [14][15][16][17][18][19]. In addition, for understanding of both normal and pathological physiological processes, a comprehensive investigation of the whole peptide set (peptidome) of a cell, organ, or organism at given time frame is necessary as well.…”

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confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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A Strategy for assessing peak purity of pharmaceutical peptides in reversed-phase chromatography methods using two-dimensional liquid chromatography coupled to mass spectrometry. Part II: Development of second-dimension gradient conditions

Cited by 7 publications

References 13 publications

A strategy for assessing peak purity of pharmaceutical peptides in reversed-phase chromatography methods using two-dimensional liquid chromatography coupled to mass spectrometry. Part I: Selection of columns and mobile phases

A strategy for assessing peak purity of pharmaceutical peptides in reversed-phase chromatography methods using two-dimensional liquid chromatography coupled to mass spectrometry. Part I: Selection of columns and mobile phases

Advances in Therapeutic Peptides Separation and Purification

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

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