A strategy for integrated pharmacokinetic study of cardiovascular herbal medicines based on chemiluminescence and HPLC-MS/MS assays: a case using Danshen injection

Abstract

A strategy for an integrated PK study based on chemiluminescence assays for cardiovascular herbal medicines with antioxidants as the main effective substances.

Identification of Pharmacokinetic Markers for Guanxin Danshen Drop Pills in Rats by Combination of Pharmacokinetics, Systems Pharmacology, and Pharmacodynamic Assays

“…To obtain easy PK parameters, we proposed to carry out the integrated PK studies by using the identified PK markers according to previous reports, viz . drug concentration sum method (Yao et al, 2017b) and AUC weighting method (Lu et al, 2008). The total concentrations of the three PK markers in rat plasma were calculated by the two methods at the studied PK time points, respectively.…”

“…The tests were carried out according to our previous report (Yao et al, 2017b) with slight modification. Briefly, mice were randomly allocated into eleven groups ( n = 8).…”

“…The resulted total concentration-time curve was prepared by plotting the PK time point against the relative sum concentration. In addition, AUC weighting integrated analysis for the three markers was performed according to the previous reports (Lu et al, 2008; Hao et al, 2009; Yao et al, 2017b).…”

An Integrated Pharmacokinetic Study of an Acanthopanax senticosus Extract Preparation by Combination of Virtual Screening, Systems Pharmacology, and Multi-Component Pharmacokinetics in Rats

“…Accordingly, considering all these factors, the six main ingredients, 4-CQA, GPS, 5-CQA, PCA, EB, and 3-CQA should be selected as drug markers to profile the in vivo behavior of ASEP. All these provide supportings to characterize the whole in vivo process of ASEP by integrating the plasma drug concentration of these ingredients using simple methods, like "plasma drug concentration sum method" and the "AUC weighting integrated method" (Yao et al, 2017;Shi et al, 2018;Yao et al, 2018;Huang et al, 2019). Therefore, we next calculated the total concentrations of the markers in rat plasma according to the previous reports.…”

“…It is based on the multi-components PK, as well as takes into account the pharmacological effects and the contribution weights of the multi-components to the treatment effects. Thus, the integrated PK parameters could be helpful for the dosage regimen design of complex TCMs' preparations (Yao et al, 2017;Shi et al, 2018;Yao et al, 2018;Huang et al, 2019). In the evaluation of efficacy, besides in vivo and in vitro experiments, systems pharmacology has received more and more attention due to its serviceability on predicting the therapeutic effects and mechanisms of multiple ingredients in TCMs (Fu et al, 2014;Liu et al, 2016;Zhang et al, 2016).…”