A strategy for introducing an endangered plant Mosla hangchowensis to urban area based on nitrogen preference

Fan, Meiying; Pan, Kaixuan; Han, Wenjuan; Lu, Yujun; Shen, Qi; Wang, Meng; Ren, Yuan; Qu, Zelong; Chang, Jie; Ge, Ying

doi:10.1007/s11738-016-2278-4

Cited by 3 publications

(1 citation statement)

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“…ex situ conservation program and complement in situ conservation carried out in nature reserves. There are examples of plants threatened with extinction introduced into the urban environment, for examples, Mosla hangchowensis [ 52 ] or Calycanthus chinensis [ 53 ]. Another interesting example is the population of the rare plant Sternbergia colchicifora found along a busy road section in the downtown of the county seat Veszprém (Hungary).…”

Section: Discussionmentioning

confidence: 99%

Long-term organogenic callus cultivation of Ranunculus illyricus L.: a blueprint for sustainable ex situ conservation of the species in urban greenery

Kocot,

Nowak,

Sitek

2024

BMC Plant Biol

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The growing trend of introducing wild plant species into urban environments necessitates the identification of novel species adapted to prevailing conditions. A promising reservoir of such species may be xerothermic communities where Ranunculus illyricus occurs. This study aimed to establish a micropropagation protocol for R. illyricus using indirect organogenesis. The protocol includes initiation of culture from various explants, callus proliferation, shoot regeneration, multiplication, and concurrent rooting. Callus appeared on most types of vegetative explants tested, but stolons were considered the best due to their good availability, high disinfection (85%), and robust callus production (maximum increase − 363.1%). The growth rate of the callus fresh matter (CFM) obtained from stolons was calculated. Greater CFM was obtained on the medium with the supplemented picloram 8.0 mg L− 1 with kinetin 5.0 mg L− 1 and in second part of experiment on medium with the addition of 2,4-D (2,4-dichlorophenoxyacetic acid) 2.0 mg L− 1 alone or picloram 6.0 mg L− 1 with kinetin 8.0 mg L− 1. Shoot organogenesis was observed on macronutrients B5 (Gamborg medium), micronutrients MS (Murashige and Skoog) medium with the addition of 2.0 mg L− 1 IBA (indole-3-butyric acid) and 4.0 mg L− 1 BAP (6-benzylaminopurine). To document the process of callus differentiation, microscopic preparations were prepared. Subsequently, the regenerated plants underwent acclimatisation and their growth in an ex situ collection was monitored over three growing seasons. In particular, in vitro-origin plants exhibited developmental patterns similar to those of their seed-origin counterparts. The incorporation of R. illyricus into urban landscapes not only increases aesthetic appeal, but also ensures the preservation of valuable genetic resources for this rare species, potentially contributing to effective ex situ conservation in the future. This marks the first scientific report on in vitro cultures of R. illyricus.

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Section: Discussionmentioning

confidence: 99%