A stringent validation of mouse adipose tissue identity markers

Jong, Jasper de; Larsson, Ola; Cannon, Barbara; Nedergaard, Jan

doi:10.1152/ajpendo.00023.2015

Cited by 245 publications

(235 citation statements)

References 53 publications

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“…In addition, the validity of brown and beige markers has not been well documented. A recent study shows that only ZIC1 but not LHX8 proves to be the marker of brown fat as well as brown adipocytes (de Jong et al 2015). Therefore, more studies are needed to define and characterize the human BAT, a potential therapeutic target for the treatment of obesity.…”

Section: Characteristics Of Adipocytesmentioning

confidence: 99%

Adipose tissue in control of metabolism

Luo

Liu

2016

Journal of Endocrinology

505

331

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Adipose tissue plays a central role in regulating whole-body energy and glucose homeostasis through its subtle functions at both organ and systemic levels. On one hand, adipose tissue stores energy in the form of lipid and controls the lipid mobilization and distribution in the body. On the other hand, adipose tissue acts as an endocrine organ and produces numerous bioactive factors such as adipokines that communicate with other organs and modulate a range of metabolic pathways. Moreover, brown and beige adipose tissue burn lipid by dissipating energy in the form of heat to maintain euthermia, and have been considered as a new way to counteract obesity. Therefore, adipose tissue dysfunction plays a prominent role in the development of obesity and its related disorders such as insulin resistance, cardiovascular disease, diabetes, depression and cancer. In this review, we will summarize the recent findings of adipose tissue in the control of metabolism, focusing on its endocrine and thermogenic function.

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Section: Characteristics Of Adipocytesmentioning

confidence: 99%

Adipose tissue in control of metabolism

Luo

Liu

2016

Journal of Endocrinology

505

331

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“…Fabp4 expression has been reported to be induced upon rosiglitazone treatment in primary cultures from various adipose depots. 16,21,35 No differences between FBS and NCS were observed (Figure 4D). …”

Section: Resultsmentioning

confidence: 92%

“…The mice were then euthanized using CO 2 , followed by cervical dislocation. The interscapular (iBAT), cervical (cBAT) and axillary brown adipose tissue (aBAT) depots were pooled (see description in 35 ). Cells were prepared for cell culture as described in.…”

Section: Methodsmentioning

confidence: 99%

Promotion of lipid storage rather than of thermogenic competence by fetal versus newborn calf serum in primary cultures of brown adipocytes

2018

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Much current understanding of brown adipocyte development comes from in-vitro cell models. Serum type may affect the behavior of cultured cells and thus conclusions drawn. Here, we investigate effects of serum type (“fetal bovine” versus “newborn calf”) on responses to differentiation inducers (the PPARγ agonist rosiglitazone or the neurotransmitter norepinephrine) in cultured primary brown adipocytes. Lipid storage was enhanced by fetal versus newborn serum. However, molecular adipose conversion (Pparg2 and Fabp4 expression) was not affected by serum type. Rosiglitazone-induced (7-days) expression of thermogenic genes (i.e. Ucp1, Pgc1a, Dio2 and Elovl3) was not systematically affected by serum type. However, importantly, acute (2 h) norepinephrine-induced thermogenic gene expression was overall markedly higher (and adipose genes somewhat lower) in cells cultured in newborn serum. Thus, newborn serum promotes thermogenic competence, and the use of fetal serum in brown adipocyte cultures (as is often routine) counteracts adequate differentiation. Agents that counteract this inhibition may therefore confoundingly be ascribed genuine thermogenic competence-inducing properties.

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“…14,15 One of the studies also claims that human brown adipose tissue abundantly expresses genes unique to BRITE/beige cells, while lacking the classical brown adipocyte-selective genes. The brown fat tissue primarily functions for energy consumption, whereas white adipose tissue mainly works to store energy.…”

Section: Potential Role Of Epsti1 In Adipose Metabolism and Energy Homentioning

confidence: 99%

“…In addition to its role in cancer, EPSTI1 has also been implicated in immune privilege, 1,7,8 autoimmune disorders, 9,10 fetal development, 11,12 apoptosis regulation, 13 adipose tissue metabolism, 14,15 and hepatitis virus infection. 16,17 Importantly, all these biological processes appear to be associated with the stromal or inflammatory factors, such as interferon alpha (IFN-α) and transforming growth factor beta (TGF-β).…”

Section: Introductionmentioning

confidence: 99%

Implications of Epithelial–Stromal Interaction 1 in Diseases Associated with Inflammatory Signaling

Gray

Zhao

2016

Cell Communication Insights

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Epithelial-stromal interaction 1 (EPSTI1) was initially identified as an induced gene in breast cancer epithelial cells by cocultured stromal fibroblasts. This discovery led to further investigation and understanding of the role of EPSTI1 in cancer. Aberrant elevation of EPSTI1 occurs primarily in invasive breast cancer epithelial cells. Forced overexpression of EPSTI1 in noninvasive cancer cells can substitute for the stromal fibroblasts. EPSTI1 was further implicated in cancer by our most recent study that identified it as one of the few most upregulated genes in human breast cancer by Krüppel-like factor 8 (KLF8), a pro-cancerous transcription factor in many cancer types. Our study also demonstrated that EPSTI1 interacts with valosin-containing protein to promote the degradation of nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) inhibitor alpha, leading to the activation and nuclear translocation of NF-κB. Additionally, EPSTI1 was shown to inhibit apoptosis by inactivating caspase 8. Studies on hepatitis C and E viruses have indicated that EPSTI1 plays a role in inhibition of the viral replication by promoting the expression of protein kinase R or protein kinase RNA-activated, a viral response gene, suggesting a role of EPSTI1 in immune response. Interestingly, in addition to transducing stromal signals, EPSTI1 has been implicated in immune privilege and autoimmune diseases, including systemic lupus erythematosus, rheumatoid arthritis, and histiocytic necrotizing lymphadenitis. This review seeks to comb EPSTI1-related studies as it was cloned a dozen years ago with a particular focus on the mechanisms of its regulation and signaling, as well as its potential roles in the diseases.

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A stringent validation of mouse adipose tissue identity markers

Abstract: de Jong JM, Larsson O, Cannon B, Nedergaard J. A stringent validation of mouse adipose tissue identity markers.

Cited by 245 publications

References 53 publications

Adipose tissue in control of metabolism

Adipose tissue in control of metabolism

Promotion of lipid storage rather than of thermogenic competence by fetal versus newborn calf serum in primary cultures of brown adipocytes

Implications of Epithelial–Stromal Interaction 1 in Diseases Associated with Inflammatory Signaling

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A stringent validation of mouse adipose tissue identity markers

Abstract: de Jong JM, Larsson O, Cannon B, Nedergaard J. A stringent validation of mouse adipose tissue identity markers.

Cited by 245 publications

References 53 publications

Adipose tissue in control of metabolism

Adipose tissue in control of metabolism

Promotion of lipid storage rather than of thermogenic competence by fetal versus newborn calf serum in primary cultures of brown adipocytes

Implications of Epithelial&ndash;Stromal Interaction 1 in Diseases Associated with Inflammatory Signaling

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Product

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Implications of Epithelial–Stromal Interaction 1 in Diseases Associated with Inflammatory Signaling