A Structural View of Egg Coat Architecture and Function in Fertilization1

Monné, Magnus; Jovine, Luca

doi:10.1095/biolreprod.111.092098

Cited by 78 publications

(79 citation statements)

References 89 publications

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“…Structural changes in the zona matrix have been observed during egg development and post fertilization in a variety of animals3738. However, in the absence of high-resolution structural information on ZP filaments, we lack a complete understanding of the structural basis of gamete interaction.…”

Section: Discussionmentioning

confidence: 99%

The C-terminal region of OVGP1 remodels the zona pellucida and modifies fertility parameters

Algarra

Han

Soriano‐Úbeda

et al. 2016

Sci Rep

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OVGP1 is the major non-serum glycoprotein in the oviduct fluid at the time of fertilization and early embryo development. Its activity differs among species. Here, we show that the C-terminal region of recombinant OVGP1 regulates its binding to the extracellular zona pellucida and affects its activity during fertilization. While porcine OVGP1 penetrates two-thirds of the thickness of the zona pellucida, shorter OVGP1 glycoproteins, including rabbit OVGP1, are restricted to the outer one-third of the zona matrix. Deletion of the C-terminal region reduces the ability of the glycoprotein to penetrate through the zona pellucida and prevents OVGP1 endocytosis. This affects the structure of the zona matrix and increases its resistance to protease digestion. However, only full-length porcine OVGP1 is able to increase the efficiency rate of in vitro fertilization. Thus, our findings document that the presence or absence of conserved regions in the C-terminus of OVGP1 modify its association with the zona pellucida that affects matrix structure and renders the zona matrix permissive to sperm penetration and OVGP1 endocytosis into the egg.

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Section: Discussionmentioning

confidence: 99%

The C-terminal region of OVGP1 remodels the zona pellucida and modifies fertility parameters

Algarra

Han

Soriano‐Úbeda

et al. 2016

Sci Rep

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“…In human ZP assembly, ZP2, ZP3, and ZP4 form long filaments cross-linked by ZP1. 15,16 The p.Ala134Thr substitution affects the ZP domain of ZP3 ( Figure 1C), which is characterized by the presence of eight conserved cysteine residues and is important for protein-protein interactions. 17,18 The amino acid substitution caused by the mutation resulted in conversion of an alanine residue to a threonine.…”

Section: Efs Can Be Classified As Either False Efs (Fefs) or Genuine mentioning

confidence: 99%

A Recurrent Missense Mutation in ZP3 Causes Empty Follicle Syndrome and Female Infertility

Chen

Bian

Liu

et al. 2017

The American Journal of Human Genetics

103

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Empty follicle syndrome (EFS) is defined as the failure to aspirate oocytes from mature ovarian follicles during in vitro fertilization. Except for some cases caused by pharmacological or iatrogenic problems, the etiology of EFS remains enigmatic. In the present study, we describe a large family with a dominant inheritance pattern of female infertility characterized by recurrent EFS. Genome-wide linkage analyses and whole-exome sequencing revealed a paternally transmitted heterozygous missense mutation of c.400 G>A (p.Ala134Thr) in zona pellucida glycoprotein 3 (ZP3). The same mutation was identified in an unrelated EFS pedigree. Haplotype analysis revealed that the disease allele of these two families came from different origins. Furthermore, in a cohort of 21 cases of EFS, two were also found to have the ZP3 c.400 G>A mutation. Immunofluorescence and histological analysis indicated that the oocytes of the EFS female had degenerated and lacked the zona pellucida (ZP). ZP3 is a major component of the ZP filament. When mutant ZP3 was co-expressed with wild-type ZP3, the interaction between wild-type ZP3 and ZP2 was markedly decreased as a result of the binding of wild-type ZP3 and mutant ZP3, via dominant negative inhibition. As a result, the assembly of ZP was impeded and the communication between cumulus cells and the oocyte was prevented, resulting in oocyte degeneration. These results identified a genetic basis for EFS and oocyte degeneration and, moreover, might pave the way for genetic diagnosis of infertile females with this phenotype.During in vitro fertilization (IVF) treatment, oocyte retrieval is performed after ovarian stimulation via vaginal puncture. Cumulus-oocyte complexes (COCs), which consist of cumulus cells surrounding the centrally located oocyte, are isolated from the individual's follicular fluid. As was first described by Coulam et al. in 1986, empty follicle syndrome (EFS) is a condition in which the ovarian response to stimulation and follicular development seems normal but no oocytes are retrieved for fertilization.1 EFS can be classified as either false EFS (FEFS) or genuine EFS (GEFS). FEFS is mainly caused by pharmacological or iatrogenic problems; however, the etiology of GEFS, which is responsible for about 33% of EFS, still remains enigmatic. 2 It has been proposed that GEFS is caused by dysfunctional folliculogenesis, ovarian aging, or genetic factors including pericentric inversion of chromosome 2 and LHCGR (MIM: 152790) mutations. 3-7 A retrospective study of 12,359 individuals who underwent assisted reproductive technology (ART) revealed that the prevalence of GEFS was about 0.016%. 8 Without oocytes for fertilization, these individuals fail to achieve pregnancy after a demanding and expensive medical intervention, resulting in stress to both physicians and the individuals themselves.9

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“…A similar matrix is found in other vertebrates, including birds, amphibians, and fish [1][2][3][4][5]. The specific functions of ZP are highly determined by its morphologic structure, which is directly related to its composition [6]. However, instead of being an evolutionarily conserved structure, this matrix is not constituted by the same proteins in all species.…”

Section: Introductionmentioning

confidence: 56%