A study by scanning electron microscopy of the bladder epithelium of the guinea pig

Wong, Yi‐Lee; Martin, B. F.

doi:10.1002/aja.1001500203

Cited by 18 publications

(13 citation statements)

References 13 publications

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“…The view that the very small cell profiles occasionally seen in the adult bladder by SEM are those of cells emerging from the pyriform into the surface cell layer [ Wolf, 1966;Wong and Martin, 1977a] is further substantiated by the present study. These profiles are more numerous in the fetal period and sometimes appear in groups, so that they are more readily encountered in the TEM preparations.…”

Section: Discussionsupporting

confidence: 72%

“…It seemed likely, therefore, that the small profiles seen by SEM are those of the upper pyriform cells entering the surface layer; a view which was also put forward by Wolf [1966], It was suggested [ Wong and Martin, 1977a] that the presence of only short mi crovilli as a surface feature of these cells indicates a lack of maturity. This was further borne out by a study of the repair processes following artificial ulceration of the epithelium [ Wong and Martin, 1977b].…”

Section: Introductionmentioning

confidence: 87%

“…[ Wong and Martin, 1977a], it was shown that the large surface cells of the lining In a recent scanning electron-microscopic transitional epithelium have pentagonal or (SEM) study on the adult guinea pig bladder hexagonal outlines, and their luminal sur-faces are characterized by a system of ridges, arranged in a reticular pattern. Among the large cells, small cell profiles are occasion ally seen which have less than five sides and their surfaces are characterized by short microvilli.…”

Section: Introductionmentioning

confidence: 99%

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Development and Maturation of the Bladder Epithelium of the Guinea Pig

Martin

Wong²

1981

Cells Tissues Organs

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In the fetal guinea pig, transitional epithelium develops at a little beyond midterm of gestation from a single layer of columnar cells. Some of the cells elongate and overlap their neighbors, but remain attached to the basement membrane by fine cytoplasmic stalks. The fetal epithelial cells do not markedly vary in size. Within the basal layer, which is the site of mitotic activity, many cells are large and binucleate, which suggests that basal cell fusions occur. It is believed that these cells eventually become surface cells. The luminal membrane develops its asymmetric structure (asymmetric unit membrane; AUM) following the appearance of AUM-bound vesicles in the apical cytoplasm, indicating that they are the source of the luminal AUM. The vesicles originate in the Golgi cisternae and are initially cup-shaped. The immature surface cells are characterized by microvilli of varying density on the luminal surface. The characteristic mature surface ridges appear to develop from rows of microvilli which fuse and link with other rows to form a reticular pattern. The process commences shortly after mid-gestation. By –15 to -10 days, the majority of cells have a well-developed surface pattern. Microvilli, however, remain prevalent in some small surface cells. These are believed to be the younger surface cells derived from pyriform intermediate cells, with the surface not fully differentiated. During the first 3 postnatal weeks, the basal cell population reaches the adult level and mitotic activity declines. The epithelium also acquires its adult morphology, whilst its functional maturity is indicated by the adult distribution and content of both glycogen, which is reduced, and alkaline phosphatase, which is increased. Rejection of cells occurs both antenatally and postnatally, and is probably a consequence of excess cell production.

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Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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Development and Maturation of the Bladder Epithelium of the Guinea Pig

Martin

Wong²

1981

Cells Tissues Organs

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“…Scanning electron microscopy (SEM) of critical point dried specimens, by virtue of its ability to display extensive views of cell surface architecture, has advanced the understanding of normal bladder structure (Mooney & Hinman, 1974;Noak et al, 1975;Mooney et al, 1976;Wong & Martin, 1977b) and is becoming an increasingly important tool in experimental and diagnostic bladder pathology (Jacobs et al, 1976;Hodges et al, 1977;Kahan et al, 1977;Newman & Hicks, 1977;Wong & Martin, 1977a;Shirai et al, 1977Shirai et al, , 1978Nishi, 1978).…”

Section: Introductionmentioning

confidence: 99%

A simple technique for the examination of urothelial surface morphology by transmission electron microscopy: platinum‐carbon replicas of critical point dried bladder tissue

Severs

1980

Journal of Microscopy

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A simple technique is described for the examination of bladder luminal surface morphology by transmission electron microscopy (TEM). Pieces of bladder tissue are critical point dried and replicated with platinum-carbon in the manner standard for freeze-fiactue. The large intact areas of replica produced show improved resolution of detail compared with scanning electron microscopy (SEM), and allow the survey of larger expanses of membrane than is possible with standard TEM techniques. Comparison of the structure observed in platinum-bon replicas of critical point dried tissue with that seen in frozen-surface replicas, fieeze-fkture replicas and thin-sections reveals the presence of artefacts in the critical point dried specimens which are not detected by SEM. Similar artefacts are evident in replicas of partially fieeze-dried samples. The nature and origin of these artefacts are discussed. It is concluded that replicas of critical point dried specimens provide useful information on surface morphology at magnif~cations intermediate between those normally used with standard TEM and SEM techniques. The details of surface morphology viewed by this method are discussed with particular reference to the structure of the intercellular boundary.

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“…Although the microscopic appearance of the lumi nal surface of transitional epithelium has been described, there is little information concerning the sur face morphology of this tissue [Porter and Bonneville, 1964;Hicks, 1965], Some studies using scanning elec tron microscopy in the guinea pig show a well-ordered arrangement of the epithelial cells [ Wong and Martin, 1977). This study was undertaken to determine a corre lation, in the rat, between the surface morphology and the previously described epithelial cells [Hicks, 1965],…”

Section: Introductionmentioning

confidence: 99%

The urinary bladder epithelium of the rat

Malczak¹

1980

Cells Tissues Organs

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The surface of the urinary bladder of the rat appeared to have a very well-ordered arrangement when viewed with the help of the scanning electron microscope. Intercellular junctions were well demarcated by prominent ridges. The majority of cells appeared to have a pentagonal or hexagonal shape. The surface morphology of the epithelial cells appeared to be consistent throughout the whole population of cells. It was made up of small ridges and some short microvilli. Comments on the significance of the surface specialization were made.

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A study by scanning electron microscopy of the bladder epithelium of the guinea pig

Cited by 18 publications

References 13 publications

Development and Maturation of the Bladder Epithelium of the Guinea Pig

Development and Maturation of the Bladder Epithelium of the Guinea Pig

A simple technique for the examination of urothelial surface morphology by transmission electron microscopy: platinum‐carbon replicas of critical point dried bladder tissue

The urinary bladder epithelium of the rat

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