A study of artificial fibre bag technique for determining the dig estibility of feeds in the rumen

Mehrez, A. Z.; Ørskov, E. R.

doi:10.1017/s0021859600037321

Cited by 677 publications

(310 citation statements)

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“…Previous authors have studied either phosphorus availability or phosphorus use by rumen microbes using radio labelled phosphorus [36], in vitro devices and in vivo trials [3,37]. The aim of the present work was to measure the rumen phosphorus release from feedstuffs commonly used in ruminant diets by the nylon bag technique [17,19] as previously done on forages [5,6,26].…”

Section: Introductionmentioning

confidence: 99%

Ruminal phosphorus availability from several feedstuffs measured by the nylon bag technique

Bravo

Meschy

Bogaert³

et al. 2000

Reprod. Nutr. Dev.

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-The present study was aimed at determining rumen phosphorus availability of some feedstuffs assessed with the nylon bag technique: forage (alfalfa), cereals (control-C wheat, formaldehyde treated-FT wheat, barley, corn), cereal by-products (wheat bran, wheat distillers, corn distillers) and meals (C and FT soya bean meals, rapeseed meals and sunflower meals). Rumen phosphorus availability was not uniform amongst the feedstuffs, varying from 33.1% (FT rapeseed meal) to 84.7% (C wheat). Alfalfa phosphorus release kinetics showed high bacterial phosphorus contamination. Technological treatments affected phosphorus content of by-products by either increasing (wheat bran and distillers) or decreasing (corn distillers) after germ extraction from the seed. Formaldehyde treatment decreasing rumen phosphorus availability (from 77.2% vs. 89.4% for wheat to 33.1% vs. 64.4% for the rapeseed meal) may depreciate the phosphorus nutritional value of FT meals. availability / feedstuff / phosphorus / rumenRésumé -Disponibilité ruminale du phosphore de certaines matières premières mesurée à l'aide de la méthode des sachets nylon. Cet article décrit la détermination, par la méthode des sachets nylon, de la disponibilité ruminale du phosphore d'un fourrage (luzerne déshydratée), de céréales (blé, blé tanné, orge, maïs), de sous-produits de céréales (son et drêches de blé, drêches de maïs) et de tourteaux (soja et soja tanné, colza et colza tanné, tournesol et tournesol tanné). La disponibilité ruminale du phosphore est très variable : de 33,1 % (tourteau de colza tanné) à 84,7 % (blé). La cinétique de libération du phosphore de la luzerne indique une importante contamination par le phosphore bactérien. Les traitements technologiques influencent la teneur en phosphore. L'extraction du germe enrichit (son et drêches de blé) ou appauvrit (drêche de maïs) en phosphore les sous-produits par rapport à la céréale. Le tannage au formol, diminuant la disponibilité ruminale du phosphore (de 77,2 % vs. 89,4 %, blé à 33,1 % vs. 64,4 %, tourteau de colza), semble pénaliser la valeur nutritionnelle du phosphore des tourteaux tannés. disponibilité / matière première / phosphore / rumen

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Section: Introductionmentioning

confidence: 99%

Ruminal phosphorus availability from several feedstuffs measured by the nylon bag technique

Bravo

Meschy

Bogaert³

et al. 2000

Reprod. Nutr. Dev.

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“…The rumen hgestion kinetics of the alfalfa hay was evaluated using the dacron bag technique (Mehrez and Orskov, 1977). Alfalfa hay (only leaves) had 95% DM, 25% crude protein (CP) and 20% NDF and was chopped in a meat mincer with lcm diameter cribs.…”

Section: Methodsmentioning

confidence: 99%

Ruminal Forage Dry Matter, Crude Protein and Fiber Digestion in Steers Fed Alfalfa Hay and Supplemented with Fresh Onion Bulbs or Cracked Corn

Pasinato¹,

Sevilla²,

García³

1999

Journal of Applied Animal Research

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“…The quantitative assessment of the breakdown process is key to carry out an effective and sustainable nutrition of the productive ruminants (Givens et al, 2000;Monteny, 2000, quoted by Van Duinkerken et al, 2005. The in situ technique developed by Mehrez and Ørskov (1977) is widely used to study the protein breakdown of the feeds in the rumen, nevertheless, several criticisms have weakened the merits that were originally assigned to this methodology, forcing several corrections needed to adjust the data according to their sources of error (Mathis et al, 2001;Cone et al, 2004). Research has also been developed on the proteins degradability with commercial enzymes (Pichard and Van Soest, 1977;Assoumani et al, 1992), where proteases from Streptomyces griseus fungus have been used, reaching satisfactory levels of proteins breakdown.…”

Section: Introductionmentioning

confidence: 99%

In vitro protein breakdown by enzyme extracts of rumen origin: comparison with methods in situ and proteases of Streptomyces griseus

Velásquez

Pichard

2010

Cienc. Inv. Agr.

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A. Velásquez, and G. Pichard. 2010. In vitro protein breakdown by enzyme extracts of rumen origin: comparison with methods in situ and proteases of Streptomyces griseus. Cien. Inv. Agr. 37(3): 57-70. Proteolytic activity of enzymatic extracts generated from rumen microorganisms cultivated in vitro was evaluated. The incubation of rumen fluid used different substrates to generate a higher enzyme concentration and promote a broad spectrum of hydrolytic activity. The composition of the substrates used in the cultivation of the fluid was enriched in protein, starch or cell wall. Enzyme preparations were evaluated by incubating in 30 mL of buffer 50 mM Tris-HCl (pH 6.5) at 39 ºC during 48 hours, 100 mg of crude protein from feeds soybean meal, canola meal, sunflower meal, gluten feed, dehydrated alfalfa meal, berseem clover, oat forage and perennial ryegrass. Enzyme extracts from cultivated rumen fluid showed an average protein breakdown of 75.5%, in eight feed samples tested. This value was very close to that measured with the technique of proteases from Streptomyces griseus (74.6% CP), but significantly lower (P≤0.05) than the one obtained by the in situ methodology (84.8% CP). The technique with extracted rumen enzymes showed higher level of proteolysis in the early hours of incubation (6 H) compared to the other techniques. These results suggest that the enzyme preparations of ruminal origin have the ability to predict degradability of feed proteins in the rumen, particularly in the first phase when most of proteins are hydrolyzed and become available for microbial utilization.

show abstract

A study of artificial fibre bag technique for determining the dig estibility of feeds in the rumen

Cited by 677 publications

References 8 publications

Ruminal phosphorus availability from several feedstuffs measured by the nylon bag technique

Ruminal phosphorus availability from several feedstuffs measured by the nylon bag technique

Ruminal Forage Dry Matter, Crude Protein and Fiber Digestion in Steers Fed Alfalfa Hay and Supplemented with Fresh Onion Bulbs or Cracked Corn

In vitro protein breakdown by enzyme extracts of rumen origin: comparison with methods in situ and proteases of Streptomyces griseus

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