A study of the central cavity in the bovine corpus luteum

Abstract

Seven hundred and six bovine corpora lutea in various luteal stages were examined morphologically and endocrinologically to discover whether there is a relationship between the presence of a central cavity in the corpus luteum and infertility in cows. A central cavity was found in 42.1 per cent (80/190) of developing corpora lutea, 33.7 per cent (126/374) of fully developed corpora lutea, 11.1 per cent (7/63) of corpora lutea in regression and in 5.1 per cent (4/79) of corpora lutea in pregnancy. There was no significant difference between the rates of appearance of midcycle follicles in corpora lutea either with or without a central cavity. The proportion of luteal cell type 1 was higher in fully developed corpora lutea with a central cavity than without, but the reverse was found with luteal cell type 2. In fully developed corpora lutea the concentration of progesterone in the luteal tissue was significantly higher in corpora lutea with a central cavity. These results suggest that there are some differences in luteal function between corpora lutea with and without a central cavity, but that the presence of a central cavity in a corpus luteum cannot be described as a pathological condition.

Summer heat stress affects prostaglandin synthesis in the bovine oviduct

“…The stages of the estrous cycle were determined based on a macroscopic observation of the ovaries and uteri (Okuda et al 1988, Miyamoto et al 2000. After trimming of the oviducts that were ipsilateral to the CL, the ampullary and isthmic sections were immediately frozen and stored at K80 8C until mRNA extraction.…”

Progesterone Stimulation by Prostaglandin F2.ALPHA. Involves the Protein Kinase C Pathway in Cultured Bovine Luteal Cells.

“…The stage of the estrous cycle was defined by macroscopic observation of the ovaries as described previously [11]. Only those CLs assigned to the mid-luteal stage (Days [8][9][10][11][12] um as a potent luteolytic agent in cattle [1]. On the other hand, the cyclic bovine corpus luteum (CL) has also been shown to be a site of PGF2α production, with higher PGF2α production apparent during the early luteal stage [2,3].…”

“…The stage of the estrous cycle was defined by macroscopic observation of the ovaries as described previously [11]. Only those CLs assigned to the mid-luteal stage (Days [8][9][10][11][12] um as a potent luteolytic agent in cattle [1].…”

Secretion of Prostaglandins E₂ and F_2α by the Bovine Endometrium in Response to Oxytocin during the Estrous Cycle

“…The luteal phase was determined by macroscopic observation of the ovary as described previously [23,24]. Uteri were washed 3 times in sterile saline containing penicillin (100 IU/ml) and streptomycin (100 µg/ml).…”