A study of the differentiation of stem cells from human exfoliated deciduous teeth on 3D silk fibroin scaffolds using static and dynamic culture paradigms

Huang, Te‐Yang; Wang, Guo-Shou; Ko, Chih-Sheng; Chen, Xiaowei; Su, Wen-Ta

doi:10.1016/j.msec.2019.110563

Cited by 6 publications

(2 citation statements)

References 31 publications

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“…To date, synthetic scaffolds [5][6][7] and natural scaffolds [8][9][10][11][12] have been widely used in 3D cell culture studies [13,14]. The parameters used in the characterization of scaffolds include degradation profiles, biomechanical properties, and biocompatibility [15].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Interaction of Adipose-Derived Mesenchymal Stem Cells with ε-Polycaprolactone and Egg White Scaffolds

Oztel¹,

Yılmaz

İşoğlu³

et al. 2023

Gazi University Journal of Science

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It is increasingly becoming important to develop three-dimensional (3-D) cell culture systems due to their numerous advantages over traditional monolayer culture. The aim of this study is to investigate the interaction of adipose derived stem cells (ADSCs) with scaffolds composed of ε-polycaprolactone (ε-PCL) and egg white. In our study; ε-PCL and egg white scaffolds were fabricated from their monomers under the catalysis of tin octoate and by polymerization by heat respectively. Characterization of PCL was carried out with Gel permeability chromatography (GPC), Fourier Transform Infrared Spectrophotometry (FTIR), Proton Nuclear Magnetic Resonance (H-NMR), Differential Scan Calorimetry (DSC) and Scanning Electron Microscopy (SEM). CM-DiI labeled ADSCs were cultured for 12 days on egg white and ε-PCL scaffolds. Cell viability was performed using MTT and nitric oxide level was evaluated for toxicity. Results showed that the number of ADSCs on egg white scaffold increased periodically throughout 12 days compared with the other groups. Although the number of ADSCs on ε-PCL scaffold increased until the 6th day of the culture, the cell number began to decrease after day 6.. These results were associated with the decomposition of PCL scaffolds that occurs through catabolic reactions. This causes the release of lactic acid which makes toxic effect on the cells. Thus, these results showed that egg white scaffold increases and protects cell adhesion and cell viability more than ε-Polycaprolactone scaffold, thus it can be used as a scaffold in tissue engineering studies that involve stem cells.

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Section: Introductionmentioning

confidence: 99%

Investigation of the Interaction of Adipose-Derived Mesenchymal Stem Cells with ε-Polycaprolactone and Egg White Scaffolds

Oztel¹,

Yılmaz

İşoğlu³

et al. 2023

Gazi University Journal of Science

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“…Tissue engineering involves cells, scaffold, and signals to which the principles of life science and engineering are applied in order to develop biological substitutes that can be implanted into the human body to restore, maintain, or improve tissue function [ 8 , 9 , 10 , 11 ]. Porous three-dimensional (3D) scaffolds, such as nanofibers fabricated by electrospinning, have greater advantages in repairing large bone defects [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Polycaprolactone/Polyethylene Glycol Blended with Dipsacus asper Wall Extract Nanofibers Promote Osteogenic Differentiation of Periodontal Ligament Stem Cells

et al. 2021

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Dipsacus asper wall (DA) is an ancient Chinese medicinal material that has long been used to maintain the health of human bones. The present study aimed to evaluate the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) of Dipsacus asper wall extracts (DAE). Microwave-assisted alcohol extraction of 100 mesh DA powder under optimal conditions can obtain 58.66% (w/w) yield of the crude extract. PDLSCs have excellent differentiation potential. PDLSCs treated with DA extract (DAE) underwent osteogenesis, exhibiting a higher expression of the Col-1, ALP, Runx2, and OCN genes, and had a 1.4-fold increase in mineralization, demonstrating the potential of DAE to promote osteogenic differentiation. After the addition of PI3K inhibitor LY294002, the expression of osteogenic genes was significantly inhibited, confirming that PI3K is an important pathway for DAE to induce osteogenesis. Mix DAE with polycaprolactone/polyethylene glycol (PCL/PEO) to obtain nanofibers with a diameter of 488 nm under optimal electrospinning conditions. The physical property analysis of nanofibers with and without DAE includes FTIR, mechanical strength, biodegradability, swelling ratio and porosity, and cell compatibility. When cells induced by nanofibers with or without DAE, the mineralization of PDLSCs cultured on PCL/PEO/DAE was 2.6-fold higher than that of PCL/PEO. The results of the study confirm that both DAE and PCL/PEO nanofibers have the effect of promoting osteogenic differentiation. In order to obtain the best induction effect, the optimal amount of DAE can be discussed in future research.

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The application of three-dimensional cell culture in clinical medicine

Qiao

Wang

2020

Biotechnol Lett

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A study of the differentiation of stem cells from human exfoliated deciduous teeth on 3D silk fibroin scaffolds using static and dynamic culture paradigms

Cited by 6 publications

References 31 publications

Investigation of the Interaction of Adipose-Derived Mesenchymal Stem Cells with ε-Polycaprolactone and Egg White Scaffolds

Investigation of the Interaction of Adipose-Derived Mesenchymal Stem Cells with ε-Polycaprolactone and Egg White Scaffolds

Polycaprolactone/Polyethylene Glycol Blended with Dipsacus asper Wall Extract Nanofibers Promote Osteogenic Differentiation of Periodontal Ligament Stem Cells

The application of three-dimensional cell culture in clinical medicine

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