A study of the dynamics of volume changes during the period of active motility in carp,Cyprinus carpioL., spermatozoa

Abstract

Carp, Cyprinus carpio L., spermatozoa swelling in NaCl hypotonic solutions (18.8, 37.5, 75, 100 mM) was studied by the methods of photometry and resistance impulses spectroscopy (RIS). The possibility of application of the value of relative optical density of cell suspension, as a qualitative characteristics of the extent of spermatozoa swelling in the period of their movement, was demonstrated. It has been noted, that the moment when swelling starts coincides with the moment of the start of sperm motility. Movement duration, extent and time of swelling depend on the osmotic pressure of the activation solution and moreover the maximum motile period is observed during the activation by 75 mM NaCl solution.

Relationship of Motility Activation to Lipid Composition, Protein Profile, and Swelling Rate of Burbot Lota lota Spermatozoon Following Change of Temperature and Osmolality

“…Assessment of spermatozoon volume change was carried out according to Dzuba et al (2001) and Bondarenko et al (2013). Briefly, optical density of the milt suspension was measured using a spectrophotometer (SPECORD 210; Analytic Jena AG) equipped with a thermostat-controlled cell chamber in a 1 cm light path cuvette at wavelength of 500 nm.…”