A study of the effect of Bacillus thuringiensis serotype H14 (subspecies israelensis)delta endotoxin on Musca larva

Abstract

Background/aim: House flies (Musca domestica) are of major public health concern in areas with poor sanitation and hygiene conditions. Biological control through the use of parasitoids and pathogens is one of the alternatives to the use of chemical pesticides for control of insects of public health importance. Materials and methods:The effects of the delta endotoxin of Bacillus thuringiensis on house fly larval mortality were studied. Gel filtration and SDS-PAGE methods were used for separation and purification of proteins. Delta endotoxin was incubated with larvae in concentrations of 0.43 mg/mL and 0.27 mg/mL in bioassay tests. Results:The results of this study indicated protein crystal toxicity against larvae of the house fly. A concentration of 0.43 mg/mL of this toxin caused 100% mortality in house fly larvae. The LD 50 amount of these toxins was calculated as 125 µg/g. Conclusion:The results of this study suggest that the use of the protein crystal including delta endotoxin of Bacillus thuringiensis serotype H14 is an effective weapon in the biological fight against the house fly.

Biological Control of Aedes aegypti Mosquitoes Using Bacillus thuringiensis

“…Sporulated culture was harvested by centrifugation. The bacterial biomass in the form of a cake was harvested and used for the preparation of the formulations, ( Mehrabi et al, 2015).…”

Distribución potencial de Musca domestica en el municipio de Jesús María, Aguascalientes, con el uso de escenarios de cambio climático

“…Recibido: 02/08/2016 Aceptado: 05/03/2018 Introducción Musca domestica es un insecto sinantrópico, que tiene una cercana asociación con el hombre y su ambiente; se puede encontrar en cualquier lugar donde existe población humana, y además se asocia con animales de corral, como aves, ganado vacuno, equino y porcino (1) , es por ello que tiene un alto potencial en la propagación de diversas enfermedades que pueden pasar de una persona a otra, o bien del animal a la persona (2) . Se estima que en los Estados Unidos de América se gastan anualmente 1.6 millones de dólares americanos en insecticidas para el control de este parásito en granjas avícolas (3) .…”