A study of the genetic variation of the aquatic fern Marsilea quadrifolia L. preserved in botanical collections in Poland and originated from natural populations in Europe

Janiak, Agnieszka; Galej, Katarzyna; Parusel, Jerzy B.; Szarejko, Iwona

doi:10.1016/j.flora.2014.08.011

Cited by 4 publications

(1 citation statement)

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“…Increasingly smaller and fragmented populations of rare species are more sensitive to the threat of loss of genetic diversity, inbred depression or the accumulation of new harmful mutations (Frankham et al 2002 ). It is therefore necessary to examine the genetic variability of the population of endangered species, which can be the basis for the development of programs of their protection (Parusel et al 2009 ; Frankham 2010 ; Janiak et al 2014 .…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and population structure of endangered plant species Anagallis foemina Mill. [Lysimachia foemina (Mill.) U. Manns & Anderb.]

Kwiecińska-Poppe

Haliniarz

Sowa

et al. 2020

Physiol Mol Biol Plants

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Anagallis foemina L. [syn. Lysimachia foemina (Mill.) U. Manns & Anderb.] is an annual, segetal weed from the family Primulaceae , recognized as a very rare and endangered species in many European countries. The rare occurrence of this species is associated with the specificity of the habitats in which it occurs. Knowledge of genetic diversity within and between rare species populations is a crucial step for investigating the causes of extinction as well as developing effective conservation strategies. The current study undertakes the assessment of the genetic variation and population structure of Anagallis foemina L. specimens collected in south-eastern Poland, Volhynian Polesie and West Volhynian Upland based on inter-simple sequence repeats (ISSR) polymorphism. Twenty ISSR primers amplified 374 DNA fragments, of which 79% were polymorphic. The polymorphic information content values ranged from 0.230 to 0.430 with an average of 0.344. An average genetic similarity calculated based on Dice algorithm between all analysed samples was 0.635 (0.28–1.00). The AMOVA study found a significant difference (Φ pt = 0.88, P = 0.001) between Anagallis L. genotypes gathered in Volhynian Polesie (VP) and West Volhynian Upland (VU). Analysis indicated, that 89% of the variation existed among groups and 11% within groups. UPGMA analyses grouped A. foemina samples into 2 clearly separated clusters. The plants of the same geographic origin were grouped together. Principal coordinates analysis (PCoA) as well as STRUCTURE also grouped samples in consistence with the collection site, indicating close genetic affinity of plants from the same location. The observed results are typical for fragmented and isolated populations of rare species. Isolation of a small population leads to a decrease in internal genetic variation and to an increase of variation among them. In that case, the conservation of populations from each regional cluster is important to preserve biodiversity.

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Section: Discussionmentioning

confidence: 99%