A study of the human skin-whitening effects of resveratryl triacetate

Abstract

Resveratrol has a variety of bioactivities that include its anti-melanogenic effects, but its use in cosmetics has been challenging partly because of its chemical instability. Resveratryl triacetate (RTA) is a prodrug that can enhance stability. The purpose of this study was to examine the skin safety and whitening effects of RTA in human subjects. The primary skin irritation potentials of RTA and resveratrol were tested at 0.1 and 0.5 % on human subjects. Resveratrol at a concentration of 0.5 % induced weak skin irritation, whereas RTA did not induce any skin responses. The skin-whitening efficacy of a cosmetic formulation containing 0.4 % RTA was evaluated in two different test models. In the artificial tanning model, the test product and the control product were applied twice daily to the skin of the forearms of 22 human subjects after pigmentation induction by ultraviolet irradiation. Applying the test and the control products to the artificial tanning model for 8 weeks increased the individual topology angles (ITA°) by 17.06 and 13.81 %, respectively, a difference that was statistically significant (p < 0.05). In the hyperpigmentation model, the test product and the control product were applied twice daily to the faces of 21 human subjects. The averaged intensity of the hyperpigmented spots decreased by 2.67 % in the test group and 1.46 % in the control group, a difference that was statistically significant (p < 0.05). Therefore, RTA incorporated into cosmetic formulations can whiten human skin without inducing skin irritation.

Human skin gene expression: Natural (trans) resveratrol versus five resveratrol analogs for dermal applications

“…Other studies have investigated how RV analogs may improve dermal health. 14,18 However, further studies are underway in our research efforts to determine how chemical modifications of RV can positively impact dermal and other health applications.…”

“…Thus, analogs of RV have been studied for increased functionality and biological activity as active ingredients in topical. 14,18 In the present study, five RV analogs were tested and based upon the CLogP values for each analog a prediction of increased solubility was somewhat reasonable to postulate. Although, the palmitoate analog with a CLogP value at 10.3 displayed the lowest impact on the human skin gene biomarkers tested; this was potentially due to its long methylene sidechain that may have caused conformational interference.…”

“…4 However, as an effective natural compound in commercial products RV has been problematic due to its rapid metabolism, especially via oral dosing and possibly in cosmetics. 2,14 One recent report examined the RV analog, resveratryl triacetate suggested that this RV analog, with increased stability, may be incorporated into cosmetic formulations that can whiten human skin without inducing skin irritation.…”

Identification of novel antimelanogenic hexapeptides via positional scanning of a synthetic peptide combinatorial library

“…[1][2][3] Exposure of human skin to UV irradiation induces several matrix metalloproteinases (MMPs), which are strongly correlated with photoageing. [4] UV irradiation triggers skin inflammation by inducing the production of cyclooxygenase (COX)-2 and proinflammatory cytokines including interleukin (IL)-1β and IL-6 in keratinocytes.…”

“…[1,2] Peptides are potentially useful as active ingredients for cosmetics because of their bioactivity relevant to skin care and biosafety. [3,4] A method called "positional scanning synthetic peptide combinatorial library" is useful for the screening of various bioactive peptides.…”