A study of the interrelationship between the triacylglycerol and protein components of very-low-density lipoproteins using the perfused rat liver

Petersburg, St.; Madeley, A; Robinson, Donald S.

doi:10.1042/bj1500315

Cited by 20 publications

(6 citation statements)

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“…The mechanism(s) underlying a potent se rum triacylglycerol-lowering effect of dietary diacylglycérol is not clear at present. Reported data suggested that the hepatic secretion of triacylglycerol as a constituent of very-lowdensity lipoprotein is severely impaired in fasted rats [16,17], As the triacylglycerol-low ering effect of dietary diacylglycérol could be detected even in overnight-fasting rats (18 h of fasting) in the present study (experiment 1, 17 days of the feeding period), the alteration in hepatic lipoprotein synthesis may not ac count for the observed changes in serum triacylglycerol levels in rats fed diacylglycérol.…”

Section: Discussionmentioning

confidence: 69%

Dietary Diacylglycerol-Dependent Reduction in Serum Triacylglycerol Concentration in Rats

et al. 1993

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The effects of dietary diacylglycerol consisting of 1,3 (65.2%) and 1,2 species (32.6%) and triacylglycerol (rapeseed oil) on the serum and hepatic lipid profiles were compared in the rat. The fatty acid composition was similar between these dietary lipids. The dietary acylglycerols were added to the experimental diets so as to provide the same amounts of fatty acids (9.39%). Dietary diacylglycerol compared with triacylglycerol significantly reduced concentrations of serum triacylglycerol at 17 and 34 days of the feeding periods without influencing those of phospholipid and cholesterol. There were no significant differences in the concentrations of hepatic triacylglycerol, cholesterol and phospholipid between the two groups of rats at 34 days of the feeding period. In the second trial, triacylglycerol in the experimental diet was replaced by varying amounts of diacylglycerol while maintaining the fatty acid contents (9.39%). After 14 days of the feeding period, significant reductions in serum triacylglycerol levels were confirmed in the groups of rats fed the diets in which diacylglycerol fatty acids supplied more than 50% (50, 75 and 100%) of total dietary fatty acids. Thus, it was confirmed that dietary diacylglycerol compared with triacylglycerol exerts a potent serum triacylglycerol-lowering effect in the rat.

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Section: Discussionmentioning

confidence: 69%

Dietary Diacylglycerol-Dependent Reduction in Serum Triacylglycerol Concentration in Rats

et al. 1993

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“…The maintenance diet ofthe liver donors was replaced by a fructose-rich diet. (Suri et al, 1978) 3 days before the perfusion experiments in order to increase the output of VLDlipoprotein triacylglycerol and protein (Petersburg et al, 1975).…”

Section: Animalsmentioning

confidence: 99%

“…After further centrifugation under the same conditions for 3 h, the VLD lipoproteins in the top 1.5 ml of the salt gradient were again removed and dialysed against three changes of 200 vol. of 0.9 % NaCl solution containing L-leucine (20mg/litre) to remove unincorporated [3H]leucine (Petersburg et al, 1975). VLD lipoproteins isolated as described above were then concentrated to a volume of approx.…”

Section: Liver Perfusionmentioning

confidence: 99%

“…This was carried out in an isothermal cabinet at 37°C by a modification of the method of Hems et al (1966), as described by Petersburg et al (1975). The perfusion medium consisted of Krebs-Ringer bicarbonate buffer, pH 7.4 (Krebs & Henseleit, 1932), containing washed rat erythrocytes (20 %, v/v), bovine serum albumin (2.5 %, w/v), glucose (300mg/ 100ml), penicillin (11 x 105 units/IOOml) and an amino acid mixture (Tolman et al, 1973) from which leucine had been omitted.…”

Section: Liver Perfusionmentioning

confidence: 99%

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The metabolic conversion of very-low-density lipoprotein into low-density lipoprotein by the extrahepatic tissues of the rat

Suri¹,

Targ²,

Robinson³

1979

Biochemical Journal

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1. The work reported was designed to provide quantitative information about the capacity of the extrahepatic tissues of the rat to degrade injected VLD lipoproteins (very-lowdensity lipoproteins, d< 1.006) to LD lipoproteins (low-density lipoproteins, d 1.006-1.063) and to study the fate of the different VLD-lipoprotein apoproteins during the degradative process. 2. Rat liver VLD lipoproteins, radioactively labelled in their protein moieties, were produced by the perfusion of the organ and were either injected into the circulation of the supradiaphragmatic rats or incubated in rat plasma at 370C. At a time (75 min) when approx. 90% of the triacylglycerol of the VLD lipoproteins had been hydrolysed, the supradiaphragmatic rats were bled and VLD lipoproteins, LD lipoproteins and HD lipoproteins (high-density lipoproteins, d 1.063-1.21) were separated from their plasma and from the plasma incubated in vitro. The apoproteins of each of the lipoprotein classes were resolved by gel-filtration chromatography into three main fractions, designated peaks I, II and III. 3. Incubation of the liver VLD lipoproteins in plasma in vitro led to the transfer of about 30 % of the total protein radioactivity to the HD lipoproteins. This transfer mainly involved the peak-II (arginine-rich and/or apo A-I) and peak-Ill (apo C) proteins. There was also a small transfer of radioactivity (about 5 % of the total) to the LD lipoproteins. 4. Injection of the liver VLD lipoproteins into the circulation of the supradiaphragmatic rat resulted in the transfer of about 15 % of the total VLD-lipoprotein radioactivity to the LD lipoproteins. This transfer involved mainly the peak-I (apo B) proteins and accounted for about 20% of the total apo B protein radioactivity of the injected VLD lipoproteins. When the endogenous plasma VLD lipoprotein was taken into account the transfer of apo B protein was about 35 %.5. The transfer of peak-Il protein radioactivity from the VLD to the HD lipoproteins was greater in the plasma of the supradiaphragmatic rat than in the incubated plasma, suggesting that there was a net transfer of peak-II apoproteins during the VLD lipoprotein degradation. The transfer of peak-III protein radioactivity was not greater in the plasma of the supradiaphragmatic rat, but there was a loss of this radioactivity from the circulation.

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“…From these previous and pre sent observations, it is supposed that the lipogenic appearance in the liver of rats fed the low protein diet would be amplified by the lipogenic nature of fructose (8-10) as a result of excess ingestion of sucrose in these rats. In the liver of normal animals, however, it is reported that the enhanced hepatic lipogenesis is associated with the higher rate of TG release from the liver (9,(19)(20)(21).…”

Section: Discussionmentioning

confidence: 98%

Effect of dietary fats and fatty acid on liver lipid accumulation in rats fed low protein diets.

Yagasaki

Kametaka

1978

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThis study was conducted to evaluate influences of dif ferent dietary fats and fatty acid on the lipid accumulation in the liver of growing rats fed low protein diets containing purified whole egg pro tein at 5 protein calories percent (PC%). Rats were fed for 2 (experi ment 1) or 3 (experiment 2) weeks. In experiment 1, rats fed low protein diets with 0.1% soybean oil accumulated more lipids in the liver than those fed a control (20 PC%) diet with 10% soybean oil. The excess accumulation of lipids tended to be inhibited by the further addition of 9.9% soybean oil and was inhibited by 9.9% linoleic acid. In experi ment 2, the lipid content in the liver of rats fed the low protein diet with 9.9 % lard was lowered by substituting 9.9% soybean oil, safflower oil and linoleic acid for lard. However, even rats fed the low protein diet with linoleic acid accumulated still more lipids in the liver than those fed the control diet. Furthermore, the liver lipid level in rats fed low protein diets with 20% soybean oil was almost the same to that in rats fed the low protein diet with 10% soybean oil. From these results, the mecha nism of lipid accumulation in liver of the rat fed low protein diet was discussed It has been reported that growing rats fed a low protein diet containing puri fied whole egg protein show accumulation of lipids in their livers when the diet contained the protein at 5 protein calories percent (PC%) (1), and the main part of lipids accumulated is triglycerides (TG) (2). In our previous study on the in vivo incorporation of acetate-2-14C into liver lipids, the total activity of TG was

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A study of the interrelationship between the triacylglycerol and protein components of very-low-density lipoproteins using the perfused rat liver

Cited by 20 publications

References 34 publications

Dietary Diacylglycerol-Dependent Reduction in Serum Triacylglycerol Concentration in Rats

Dietary Diacylglycerol-Dependent Reduction in Serum Triacylglycerol Concentration in Rats

The metabolic conversion of very-low-density lipoprotein into low-density lipoprotein by the extrahepatic tissues of the rat

Effect of dietary fats and fatty acid on liver lipid accumulation in rats fed low protein diets.

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