A Study of the Substrate Specificity and Other Properties of the Alkaline Phosphatase of Escherichia coli

Heppel, Leon A.; Harkness, Donald R.; Hilmoe, R.J.

doi:10.1016/s0021-9258(18)60381-3

Cited by 261 publications

(30 citation statements)

References 31 publications

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“…When supernatant solutions from low-speed centrifugation of sonic extracts were centrifuged at high speeds to remove ribosomes and membrane fragments, part of the activity of all three phosphatases was observed in the pelleted material. The extent of the pelleted activity was 5 to 10%, 20 to 25%, and 30 to 40% of the total activity for the ayclic phosphodiesterase, acid hexose phosphatase, and nonspecific acid phosphatase, respectively. On Sephadex G-200 columns, nonspecific acid phos- VOL.…”

Section: Resultsmentioning

confidence: 95%

“…After the positions of the major protein and activity bands were determined on basic gels, 1-mm sections in the same mobility region were cut from unstained gel sections, and 20 ,ul of Laemmli sample buffer was added to the sections. After crushing the gel pieces in the sample buffer and boiling the mixture for 2 min, the samples were applied to SDSpolyacrylamide gels, which were run as usual.…”

Section: Methodsmentioning

confidence: 99%

“…Among the first of these enzymes to be studied was alkaline phosphatase (EC 3.1.3.1), originally purified and characterized by Garen and Levinthal (15). The enzyme hydrolyzes a wide variety of phosphate esters as well as pyrophosphate bonds (14,20). Anraku purified a cyclic 2',3'-nucleotide phosphodiesterase that also hydrolyzed 3'-nucleotides (7,8).…”

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confidence: 99%

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Resolution and purification of three periplasmic phosphatases of Salmonella typhimurium

Kier¹,

Weppelman²,

Ames³

1977

J Bacteriol

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A survey of Salmonella typhimurium enzymes possessing phosphatase or phosphodiesterase activity was made using several different growth conditions. These studies revealed the presence of three major enzymes, all of which were subsequently purified: a cyclic 2' ,3'-nucleotide phosphodiesterase (EC 3.1.4.d), an acid hexose phosphatase (EC 3.1.3.2), and a nonspecific acid phosphatase (EC 3.1.3.2). A fourth enzyme hydrolyzed bis-(p-nitrophenyl)phosphate but none of the other substrates tested. No evidence was found for the existence of an alkaline phosphatase (EC 3.1.3.1) or a specific 5'-nucleotidase (EC 3.1.3.5) in S. typhimurium LT2. All three phosphatases could be measured efficiently in intact cells, which suggested a periplasmic location; however, they were not readily released by osmotic shock procedures. The nonspecific acid phosphatase, which was purified to apparent homogeneity, yielded a single polypeptide band on both sodium dodecyl sulfate and acidic urea gel electrophoretic systems.

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Section: Resultsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

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Resolution and purification of three periplasmic phosphatases of Salmonella typhimurium

Kier¹,

Weppelman²,

Ames³

1977

J Bacteriol

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“…It has not yet known whether ALP can also split and liberate the energy present in cyclic trimetaphosphate in addition to the linear molecules in human cells, like the enzyme from E. coli [108]. An inorganic triphosphatase that hydrolyses the linear triphosphate has been reported to be present in mammalian cells [109].…”

Section: Generation Of Metabolic Energymentioning

confidence: 99%

Inorganic Polymeric Materials for Injured Tissue Repair: Biocatalytic Formation and Exploitation

et al. 2022

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Two biocatalytically produced inorganic biomaterials show great potential for use in regenerative medicine but also other medical applications: bio-silica and bio-polyphosphate (bio-polyP or polyP). Biosilica is synthesized by a group of enzymes called silicateins, which mediate the formation of amorphous hydrated silica from monomeric precursors. The polymeric silicic acid formed by these enzymes, which have been cloned from various siliceous sponge species, then undergoes a maturation process to form a solid biosilica material. The second biomaterial, polyP, has the extraordinary property that it not only has morphogenetic activity similar to biosilica, i.e., can induce cell differentiation through specific gene expression, but also provides metabolic energy through enzymatic cleavage of its high-energy phosphoanhydride bonds. This reaction is catalyzed by alkaline phosphatase, a ubiquitous enzyme that, in combination with adenylate kinase, forms adenosine triphosphate (ATP) from polyP. This article attempts to highlight the biomedical importance of the inorganic polymeric materials biosilica and polyP as well as the enzymes silicatein and alkaline phosphatase, which are involved in their metabolism or mediate their biological activity.

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“…Experimentally motivated distributions of parameter from which the function parameters are drawn [17], [23]- [24].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Oscillators for Specific Tasks Predicts Preferred Biochemical Implementations

Agrahar

Rust

2022

Preprint

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Oscillatory processes are used throughout cell biology to control time-varying physiology including the cell cycle, circadian rhythms, and developmental patterning. It has long been understood that free-running oscillations require feedback loops where the activity of one component depends on the concentration of another. Oscillator motifs have been classified by the positive or negative net logic of these loops. However, each feedback loop can be implemented by regulation of either the production step or the removal step. These possibilities are not equivalent because of the underlying structure of biochemical kinetics. By computationally searching over these possibilities, we find that certain molecular implementations are much more likely to produce stable oscillations. These preferred molecular implementations are found in many natural systems, but not typically in artificial oscillators, suggesting a design principle for future synthetic biology. Finally, we develop an approach to oscillator function across different reaction networks by evaluating the biosynthetic cost needed to achieve a given phase coherence. This analysis predicts that phase drift is most efficiently suppressed by delayed negative feedback loop architectures that operate without positive feedback.PACS numbers47.15.-x

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A Study of the Substrate Specificity and Other Properties of the Alkaline Phosphatase of Escherichia coli

Cited by 261 publications

References 31 publications

Resolution and purification of three periplasmic phosphatases of Salmonella typhimurium

Resolution and purification of three periplasmic phosphatases of Salmonella typhimurium

Inorganic Polymeric Materials for Injured Tissue Repair: Biocatalytic Formation and Exploitation

Optimizing Oscillators for Specific Tasks Predicts Preferred Biochemical Implementations

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