A study on clinical effectiveness of cosmetics containing human stem cell conditioned media

Abstract

Background: With the steadily increasing trend of functional cosmetics consumption, the public is increasingly expecting the efficacy of cosmetics, and in order to meet these consumer needs, the development of various effective raw materials, as well as products through technical convergence research, has been activating focusing on dermatology. In the Republic of Korea, much attention was paid to cosmetic raw materials using human stem cells as a new growth engine at the end of the 2000s. Accordingly, the Ministry of Food and Drug Safety implemented the Regulations on Cosmetic Safety Standards, etc., which contains the safety standards of humanderived cell and tissue culture as raw materials for cosmetics. Methods: Twice a day, morning and evening, for 28 days, 3 mL of the sample provided for each subject was applied onto the entire face using an airbrush. The subjects were divided into a control group (C), an experimental group 1 (ADSC-CM 5% group, E1) and an experimental group 2 (polymersome-containing of ADSC-CM 5% group, E2), and there were 20 subjects in each group. Results: Moisture content on the U-zone, melanin and erythema of lid-cheek, overall size and depth of eye wrinkles, and skin roughness of the items showed significant differences among all three groups. More specifically, E1 had a higher rate of skin improvement than C, and E2 had the highest skin improvement rate in all items: skin moisture content, transepidermal water loss (TEWL), melanin and erythema of lid-cheek, eye wrinkles, and skin roughness of lid-cheek. Conclusions: The effectiveness of ADSC-CM was verified in all items: increases in moisture content, decrease in TEWL, and improvements of whitening and wrinkles. Moreover, the effectiveness of ADSC-CM was observed as higher in samples of polymersome-containing ADSC-CM than those of raw material ADSC-CM. Furthermore, percutaneous absorption was improved compared with previous studies on formulations in relation to polymersome at the same concentration of ADSC-CM, thereby resulting in higher effectiveness.

In vitro effects of conditioned medium from bioreactor cultured human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on skin-derived cell lines

“…Stem cell-based cosmetics are an emerging science and are increasingly being used in regenerative medicine, offering a potential cure to various serious diseases, especially in unmet medical needs [ 1 ]. Longevity and aging societies are a global trend, and Korea is progressing rapidly into an aging society [ 2 ].…”

“…At the cellular level, stem cell dysfunction and natural wastage appear to be key drivers, and both genetic and epigenetic factors are involved in a complex interaction that over time results in the decline of our main protective interface with the external environment. Past and current understanding of the cellular and molecular intricacies of skin aging provides a foundation for future approaches designed to revert the aging phenotype [ 1 , 3 ].…”

“…This hUC-MSC conditioned medium consisting of various growth factors and cytokines is known to promote the regeneration of damaged tissue. Thus, stem cell-conditioned medium is an innovative development that can be used in skin care and hair care [ 1 ].…”

Extracellular Vesicles as Potential Theranostic Platforms for Skin Diseases and Aging

“…Although several stem cell products are currently undergoing clinical trials, there is only one type of stem cell therapy agent approved by the FDA for clinical use, possibly because of safety concerns [102]. Without safety issues of cell therapy, ADSC derivatives, including ADSC-EVs and ADSC-CM, are already accessible to the public as cosmetic agents for skin whitening, rejuvenation, and scar prevention [103,104].…”

Stem cells and the pursuit of youth, a tale of limitless possibilities and commercial fraud

“…For skin and hair, in particular, there is a considerable number of publications supporting the claim that stem cell condition media have a measurable effect when used to treat skin damage or hair loss[ 14 - 17 ]. Notably, these studies also report significant inconsistencies in the outcomes depending on the method used for cell isolation, medium collection, medium processing, and mode of application[ 18 ]. On the other hand, allogenic whole-stem cell use for cosmetic applications is still in it is infancy.…”