A study on hepatoprotective activity and antioxidant properties of Musa balbisianacolla Seeds

Abstract

This study aims to determine and characterize the antioxidant properties of Musa balbasiana colla flower and seeds and Musa Paradisiaca. Hepatoprotective potential of Musa balbisiana colla seeds was also assessed. The ethanolic extract of flower and seeds of the samples were evaluated for antioxidant assay using 1,1-diphenyl-2-picrl-hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2,2-azino-bis (3-ethlbenzothiazoline-6-sulfonic acid) (ABTS), Nitric oxide (NO) assays. The seed extract of Musa balbisiana exhibited the highest scavenging activity. Also, the highest total phenolic content (195.62µg/mg) was obtained from the seed extract. Thus, having high antioxidant activity, the seed was further evaluated for in- vitro hepatoprotective effect in paracetamol- induced hepatotoxicity in HepG2 cell line. Dose dependent effect of MbS (25, 50, 100, 200 µM) were tested on HepG2 cell line. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was used for the determination of toxicity which resulted in 80% recovery with 200µg/ml concentration in a dose dependent manner. The result indicated that the high IC50 was the important factor ensuring to posses powerful radical scavenging activity. The research conducted contributes data to validate the hepatoprotective effect of Musa balbisiana colla seeds on paracetamol -induced liver toxicity.