A study on natural recovery of tassel fertilization and doubling method in maize haploids

Jiang, Long; Yang, Xiaoyan; Li, X.Y.; Deng, Pan; Jing, Gao; Wang, X.Q.; Xing, Zhao; Zhao, Rulang

doi:10.4238/gmr16019173

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…On average, a colchicine treatment survival was 85.7% for all populations (Table 3). Lower seedling survival rates were observed in other studies, commonly amounting to 60-70% on average (Dang et al, 2012;Battistelli et al, 2013;Jiang et al, 2017).…”

Section: Resultscontrasting

confidence: 52%

The development of homozygous maize lines using an in vivo haploid induction in the Croatian germplasm

Mazur

Vila

Brkić

et al. 2019

Poljoprivreda (Online)

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The in vivo haploid induction has been widely applied to the maize breeding in recent decades, but it has not been used in the breeding programs in the Republic of Croatia by now. This study's objectives were to examine the haploid induction rates in the Croatian germplasm and to evaluate the properties of the D0 haploids, which are essential for a successful implementation of this method in breeding. The in vivo haploid induction was performed on 11 single-cross hybrids using the Zarodyshevy Marker Krasnodarsky (ZMK) inducer, and colchicine was used as a chromosome doubling agent. Emergence, misclassification rate, colchicine treatment survival, chromosome doubling rate and self-pollination success were examined in the D0 generation. The haploid induction rates ranged from 6.9 to 15.8%, which is consistent with the average induction rates characteristic of the ZMK inducer and the other modern ones. Significant differences were found among the populations of D0 haploids for all tested properties, except for self-pollination success. On average, the misclassification rates were lower, and the seedling survival rates were higher than those reported in other studies, indicating a possibility of a successful application of the doubled haploid method in maize breeding.

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Section: Resultscontrasting

confidence: 52%

The development of homozygous maize lines using an in vivo haploid induction in the Croatian germplasm

Mazur

Vila

Brkić

et al. 2019

Poljoprivreda (Online)

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“…Recent studies reported that HMF is genotype dependent (Wu et al 2016;Ma et al 2018). In Chinese germplasm, Jiang et al (2017) found minimal levels of HMF from 4% to 11% with an average of 8.28%.…”

Section: Introductionmentioning

confidence: 97%

QTL mapping of spontaneous haploid genome doubling using genotyping-by-sequencing in maize (Zea mays L.)

et al. 2020

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Genome doubling of haploids is one of the major constraints of large-scale doubled haploid (DH) technology. Improving spontaneous haploid genome doubling (SHGD) is an alternative to overcome this limitation. In this study, we aimed to construct a high-density linkage map based on genotyping by sequencing (GBS) of Single Nucleotide Polymorphism (SNPs), to detect QTL and QTL by environment (Q by E) interactions affecting SHGD, and to identify the best trait for mapping and selection of haploid male fertility (HMF). To this end, a bi-parental population of 220 F2:3 families was developed from a cross between A427 (high HMF) and CR1Ht (moderate HMF) to be used as donor. A high-density linkage map was constructed containing 4,171 SNP markers distributed over 10 chromosomes with an average distance between adjacent markers of 0.51 cM. QTL mapping for haploid fertile anther emergence (AE), pollen production (PP), tassel size (TS), and HMF, identified 27 QTL across three environments, and Q by E interactions were significant. A major QTL was identified on chromosome 5. This QTL explained over 45% of the observed variance for all traits across all environments. The introgression of this major QTL, using marker-assisted backcrossing, has great potential to overcome the need of using colchicine in DH line development.

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“…Generally, haploids are sterile, while fertile gametes could be produced when the chromosomes are doubled. Most of the current chromosome doubling methods depend on antimitotic chemical reagents, such as colchicine, amiprophos-methyl (APM) and pronamide [ 11 , 12 , 13 , 14 ]. Traditionally, colchicine treatment of haploids results in a better doubling effect.…”

Section: Introductionmentioning

confidence: 99%

QTL Mapping and Prediction of Haploid Male Fertility Traits in Maize (Zea mays L.)

Chen

et al. 2020

Plants

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Chromosome doubling of maize haploids is a bottleneck in the large-scale application of doubled haploid (DH) technology. Spontaneous chromosome doubling (SCD) of haploid has been taken as an important method in the production of DH lines and low haploid male fertility (HMF) is a main limiting factor for the use of SCD. To study its genetic basis, haploids of 119 DH lines derived from a cross between inbred lines Qi319 and Chang7-2 were used to map the quantitative trait locus (QTL) contributing to HMF. Three traits including anther emergence rate (AER), anther emergence score (AES) and pollen production score (PPS) of the haploid population were evaluated at two locations. The heritability of the three traits ranged from 0.70 to 0.81. The QTL contributing to AER, AES and PPS were identified on the chromosomes 1, 2, 3, 4, 5, 7, 9 and 10. Five major QTL, qAER5-1, qAER5-2, qAES3, qPPS1 and qPPS5, were found and each could explain more than 15% of the phenotypic variance at least in one environment. Two major QTL, qPPS1 and qPPS5, and two minor QTL, qAES2 and qAER3, were repeatedly detected at both locations. To increase the application efficiency of HMF in breeding programs, genomic prediction for the three traits were carried out with ridge regression best linear unbiased prediction (rrBLUP) and rrBLUP adding QTL effects (rrBLUP-QTL). The prediction accuracies of rrBLUP-QTL were significantly higher than that by rrBLUP for three traits (p < 0.001), which indirectly indicates these QTL were effective. The prediction accuracies for PPS were 0.604 (rrBLUP) and 0.703 (rrBLUP-QTL) across both locations, which were higher than that of AER and AES. Overall, this study provides important information to understand the genetic architecture of SCD of maize haploids.

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A study on natural recovery of tassel fertilization and doubling method in maize haploids

Cited by 3 publications

References 11 publications

The development of homozygous maize lines using an in vivo haploid induction in the Croatian germplasm

The development of homozygous maize lines using an in vivo haploid induction in the Croatian germplasm

QTL mapping of spontaneous haploid genome doubling using genotyping-by-sequencing in maize (Zea mays L.)

QTL Mapping and Prediction of Haploid Male Fertility Traits in Maize (Zea mays L.)

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