A study on the efficacy of the recombinant <i>Yersinia</i> adhesin A vaccine against yersiniosis in the early phase

Abstract

Yersinia pseudotuberculosis (Y. ptb) is a zoonotic pathogenic bacterial species of the family Enterobacteriaceae and causes yersiniosis, an acute intestinal infection in humans and animals. Y. ptb is often implicated in lethal epidemics in zoo animals and reductions in the breeding population, but a valid prevention method has not been established. Therefore, this study aimed to develop a vaccine for yersiniosis control. The immunogenicity of one of the adhesion factors involved in pathogenic mechanisms of Y. ptb, Yersinia adhesin A (YadA), was investigated. BALB/c mice were divided into 3 groups: in group 1, mice received insoluble recombinant YadA (rYadA) produced in genetically engineered Escherichia coli (100 µg/dose); in group 2, mice received inactivated Y. ptb with strong expression of YadA (20 mg/dose);and in group 3, mice received phosphate-buffered saline (0.2 ml/dose). All interventions were administered subcutaneously twice at an interval of 1 week. One week after the second administration, Y. ptb (107 cells/mouse) was inoculated orally. As a result, the survival rate was 100% in group 1, 60% in group 2, and 0% in group 3. The anti-YadA antibody titer increased in a stepwise fashion in groups 1 and 2. The present study results suggest that rYadA shows promise as a protective antigen against yersiniosis. This study concluded that vaccination against Y. ptb may become available as a new method to prevent lethal epidemics in animals.

Antioxidative, Antimicrobial Activity and HPLC Analysis of Ornithogalum pyrenaicum

“…In this study, antimicrobial properties of the plant were examined and the extract of the O. pyrenacium showed antimicrobial effects against M. smegmatis, P. aeruginosa, S. aureus, and Y. pseudotuberculosis. Y. pseudotuberculosis is a pathogen that causes symptoms such as swelling of lymph nodes, septicemia, typhoidal clinical effects in humans (27). P. aeruginosa is a gram-negative bacterium.…”

Immunogenicity of trimeric autotransporter adhesins and their potential as vaccine targets

“…They indicated that live Y. enterocolitica can (group 2), or just with phosphatebuffered saline (group 3-control). Survival rates after exposure to pathogenic Y. pseudotuberculosis were 100% (group 1), 60% (group 2), and 0% (group 3), respectively [95]. However, the recombinantly expressed YadA proteins did not induce mucosal immunity as measured by IgG secretion.…”

“…The authors concluded that YadA shows promising results as a vaccine component, but more research towards its safety, immunogenicity, and protective properties is necessary [95].…”

Genomic Analysis of Pasteurella atlantica Provides Insight on Its Virulence Factors and Phylogeny and Highlights the Potential of Reverse Vaccinology in Aquaculture

“…In fact, Tahir and Skurnik (2001) [93] found that immunizing mice with live bacteria raised antibodies against only epitopes located in the N-termini, whereas using inactivated bacteria raised antibodies against other epitopes as well. In addition, a study [94] found that mice immunized using a recombinant YadA vaccine had higher survival compared to those vaccinated with inactivated Y. pseudotuberculosis.…”