A Study on the factors affecting biomass formation by a highly kojic acid producer fungal isolate from sugarcane molasses

Zohri, Abd El-Naser; Mahmoud, Ghada; Sadik, Nermien; Hanafy, Radwa A.

doi:10.21608/esugj.2018.228291

Cited by 4 publications

(5 citation statements)

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“…After optimizing KA production by A. flavus ASU45 (OL314748) using Box-Behnken statistical design, kojic acid production values increased to 81.59 g/l (with predicted value 79.24 g/l) using glucose 150, yeast extract 5, KH 2 PO 4 1, MgSO 4 .7H 2 O 2 g/l and pH 3. In agreement with our findings; after the optimization process of KA from A. flavus the researcher reached varied quantities; Zohri et al [ 12 ] obtained 24.65 g/l KA, Sanjotha et al, [ 13 ] obtained 31 g/l KA, Rosfarizan and Ariff [ 64 ] obtained 39.9 g/l KA, Devi et al [ 63 ] obtained 47 g/l KA, El-Kady et al [ 11 ] obtained 53.5 g/l KA, and Devi et al [ 65 ] obtained 82.6 g/l of kojic acid. In our study, the kojic acid was extracted and crystalized using ethyl acetate (1:1, filtrate: solvent).…”

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

“…It was found that A. oryzae could produce 24 to 41 g/l KA in a glucose medium [15,59], however, Yan et al [53] obtained 33.1 g/l KA from A. oryzae M866 in a corn stalk medium. Also, it was found that A. flavus could produce 24 to 47 g/l KA using glucose as a carbon source [12,13,52,[60][61][62][63][64]. Statistical experimental designs were recently preferred in the optimization process over the traditional way (one factor at a time) for saving time, costs, minimizing the experimental errors, and showing the interaction between the tested parameters [17].…”

Section: Discussionmentioning

confidence: 99%

“…Kojic acid is fundamentally produced by Aspergillus flavus [9][10][11][12][13], A. flavus var. columnaris [11,14], A. oryzae [15][16][17], A. oryzae var.…”

Section: Introductionmentioning

confidence: 99%

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Production of kojic acid by Aspergillus flavus OL314748 using box-Behnken statistical design and its antibacterial and anticancer applications using molecular docking technique

Mahmoud,

Abdel Shakor,

Kamal-Eldin

et al. 2024

BMC Microbiol

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Kojic acid is a wonderful fungal secondary metabolite that has several applications in the food, medical, and agriculture sectors. Many human diseases become resistant to normal antibiotics and normal treatments. We need to search for alternative treatment sources and understand their mode of action. Aspergillus flavus ASU45 (OL314748) was isolated from the caraway rhizosphere as a non-aflatoxin producer and identified genetically using 18S rRNA gene sequencing. After applying the Box-Behnken statistical design to maximize KA production, the production raised from 39.96 to 81.59 g/l utilizing (g/l) glucose 150, yeast extract 5, KH2PO4 1, MgSO4.7H2O 2, and medium pH 3 with a coefficient (R2) of 98.45%. Extracted KA was characterized using FTIR, XRD, and a scanning electron microscope. Crystalized KA was an effective antibacterial agent against six human pathogenic bacteria (Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Serratia marcescens, and Serratia plymuthica). KA achieves high inhibition activity against Bacillus cereus, K. pneumonia, and S. plymuthica at 100 μg/ml concentration by 2.75, 2.85, and 2.85 compared with chloramphenicol which gives inhibition zones 1, 1.1, and 1.6, respectively. Crystalized KA had anticancer activity versus three types of cancer cell lines (Mcf-7, HepG2, and Huh7) and demonstrated high cytotoxic capabilities on HepG-2 cells that propose strong antitumor potent of KA versus hepatocellular carcinoma. The antibacterial and anticancer modes of action were illustrated using the molecular docking technique. Crystalized kojic acid from a biological source represented a promising microbial metabolite that could be utilized as an alternative antibacterial and anticancer agent effectively.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Kojic acid is fundamentally produced by Aspergillus flavus [9][10][11][12][13], A. flavus var. columnaris [11,14], A. oryzae [15][16][17], A. oryzae var.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production of kojic acid by Aspergillus flavus OL314748 using box-Behnken statistical design and its antibacterial and anticancer applications using molecular docking technique

Mahmoud,

Abdel Shakor,

Kamal-Eldin

et al. 2024

BMC Microbiol

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show abstract

“…When interactions between different components are present, the traditional way of medium optimization, one factor at a time, is time consuming, expensive, and frequently leads to misinterpretation of results. Statistical experimental designs reduce the amount of error in determining the influence of parameters and allow for simultaneous, systematic, and efficient manipulation of all variables [18][19][20] .…”

Section: Aphidsmentioning

confidence: 99%

Application of Aspergillus Oryzae Asu44 (Ol314732) and Their Kojic Acid as Pesticides Against Cotton Aphid, Aphis Gossypii

Mahmoud

Zohri

Kamal-Eldin

et al. 2023

Bulletin of Pharmaceutical Sciences. Assiut

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Because of their negative effects on the environment, humans, and other organisms, chemical pesticides have been prohibited in many countries. Alternative microbial management is easy-to-implement, effective, and safe for humans and the environment. Fungus-based biopesticide products like kojic acid could commercialize as effective alternative chemical pesticides. Aspergillus oryzae ASU44 (OL314732) was isolated from hollyhock rhizosphere and identified using 18S rRNA gene sequencing. The strain showed high ability of kojic acid production especially after optimizing the production using 41-run Box-Behnken statistical design. Maximum kojic acid production was 46.53 g/l (predicted values 46.62 g/l) obtained in run number (23) using Glucose (150, g/l), Yeast extract (5 g/l), KH2PO4 (3 g/l), MgSO4.7H2O (0.5 g/l) and pH (3). The design was effective to applied with coefficient (R 2 ) 0.988, 0.986 (adjusted R 2 0.975, 0.972) for kojic acid (g/l) and dry fungal mass formation (g/l). The pathogenicity of the fungus and their kojic acid in addition to standard synthetic kojic acid was investigated to the cotton aphid, Aphis gossypii Glover (Homoptera: Aphididae). All treatments had a considerable impact on aphid mortality. The mortality rate was relative to the duration of exposure and the quantities of bio-pesticides agents. However, data revealed that as concentrations increased, the lethal time values decreased. Aspergillus oryzae ASU44 and its extract containing kojic acid were found effective against A. gossypii. As a result, this research suggests that these agents could be effective in aphid cotton management.

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Statistical optimization, kinetic modeling, and techno-economic analysis for the production of high molecular mass dextran using sugarcane industrial waste-molasses

Rahman,

Pasupathi,

Karuppiah

2024

Bioresource Technology Reports

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A Study on the factors affecting biomass formation by a highly kojic acid producer fungal isolate from sugarcane molasses

Cited by 4 publications

References 0 publications

Production of kojic acid by Aspergillus flavus OL314748 using box-Behnken statistical design and its antibacterial and anticancer applications using molecular docking technique

Production of kojic acid by Aspergillus flavus OL314748 using box-Behnken statistical design and its antibacterial and anticancer applications using molecular docking technique

Application of Aspergillus Oryzae Asu44 (Ol314732) and Their Kojic Acid as Pesticides Against Cotton Aphid, Aphis Gossypii

Statistical optimization, kinetic modeling, and techno-economic analysis for the production of high molecular mass dextran using sugarcane industrial waste-molasses

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