A study on the inhibition of <scp>VEGF</scp> expression in salivary gland adenoid cystic carcinoma cells via <scp>iNOS</scp> gene <scp>RNAi </scp><i>in vitro</i>

Yang, Ke-Xiong Ou; Liang, Jun; Yang, Zhiyun; Zhao, Jianjiang

doi:10.1111/jop.12217

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…This biomarker may also be utilized to create novel antiinflammatory medications that prevent cancer and utilized as a supplement to the radiation therapy and chemotherapy. 14,15 In this study VEGF shows positive correlation with tumor grading. Even though some immunohistochemistry work did not find a association expression of VEGF in epithelial dysplasia or cancer of oral cavity, due to differences in antibodies used in identifying the several isoforms of VEGF.…”

Section: Discussionsupporting

confidence: 56%

Colocalization of VEGF and iNOS in Parenchymal and Stromal Cells of Oral Squamous Cell Carcinoma

Essa

2024

Egyptian Dental Journal

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Background and objectives: Tumor angiogenesis and inflammation perception are intricately linked to cancer progression. Vascular endothelial growth factor (VEGF) controls distinct procedures in angiogenesis of pathological conditions in metabolic functions of cancer cells with the inflammatory cytokine inducible nitric oxide synthase (iNOS) in parenchymal together with stromal cells of oral squamous cell carcinoma (OSCC) as well as assessing microvessel density (MVD).Methods: Immunohistochemistry was applied to analyze VEGF, iNOS as well as CD31 expression within 31 OSCCs paraffin blocks, which grouped into 13 well differentiated, 10 moderately differentiated, and 8 poorly differentiated (paraffin blocks OSCC).Results: Both VEGF and iNOS exhibit strong expression in both parenchymal and stromal cells in all grades of OSCC together with increased MVD. Conclusion:Enhanced expression of VEGF and iNOS together with MVD potentially correlate with OSCC grades.

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Section: Discussionsupporting

confidence: 56%

Colocalization of VEGF and iNOS in Parenchymal and Stromal Cells of Oral Squamous Cell Carcinoma

Essa

2024

Egyptian Dental Journal

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“…Angiogenesis plays an important role in cancer development and metastasis. Molecules involved in this process, such as VEGF, iNOS and NF-κB were noted to be highly expressed in ACC cells as compared to normal salivary gland cells [ 63 , 64 ]. VEGF, c-kit, and EGFR were not correlated with ACC recurrence of prognosis in one study [ 48 ].…”

Section: Molecular Pathology Of Adenoid Cystic Carcinomamentioning

confidence: 99%

Adenoid cystic carcinoma: current therapy and potential therapeutic advances based on genomic profiling

et al. 2015

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Adenoid cystic carcinoma (ACC) is a rare cancer with high potential for recurrence and metastasis. Efficacy of current treatment options, particularly for advanced disease, is very limited. Recent whole genome and exome sequencing has dramatically improved our understanding of ACC pathogenesis. A balanced translocation resulting in the MYB-NFIB fusion gene appears to be a fundamental signature of ACC. In addition, sequencing has identified a number of other driver genes mutated in downstream pathways common to other well-studied cancers. Overexpression of oncogenic proteins involved in cell growth, adhesion, cell cycle regulation, and angiogenesis are also present in ACC. Collectively, studies have identified genes and proteins for targeted, mechanism-based, therapies based on tumor phenotypes, as opposed to nonspecific cytotoxic agents. In addition, although few studies in ACC currently exist, immunotherapy may also hold promise. Better genetic understanding will enable treatment with novel targeted agents and initial exploration of immune-based therapies with the goal of improving outcomes for patients with ACC.

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“…17 On the one hand, iNOS mediates the proliferation and up-regulation of VEGF through its product of NO, which promotes the growth of tumors. 18 , 19 On the other hand, iNOS promotes tumor angiogenesis, proliferation, differentiation, and growth by up-regulating MMPs and P53 oncogene. 20 HIF-α is an important reverting factor that maintains the oxygen balance in the body and is a gene subunit that regulates the oxygen balance of tissues.…”

Section: Discussionmentioning

confidence: 99%

Arterial instillation of rapamycin in treatment of rabbit hepatic xenograft tumors and its effects on VEGF, iNOS, HIF-1α, Bcl-2, Bax expression and microvessel density

Ye-xing

Sun

et al. 2021

Science Progress

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Hepatocellular carcinoma is one of the leading causes of malignant tumor related death word wide with poor prognosis. Chemotherapy and TACE are main treatment methods for advanced stage cases. Rapamycin, a macrolide compound that initially used to coat coronary stents, can inhibit the growth of a variety of cancer cells especially hepatocellular carcinoma. Twenty-four healthy adult New Zealand white rabbits underwent CT-guided puncture to prepare a model of VX2 liver xenograft tumor. The rabbits were randomly divided into four groups with six in each group and received the following treatments: APR-TACE1: arterial perfusion of high-dose rapamycin combined with TACE; APR-TACE2: arterial perfusion of low-dose rapamycin combined with TACE; TACE: TACE alone; and IVR-TACE: intravenous injection of rapamycin combined with TACE. Two weeks after TACE treatment, the rabbits received CT scan and DSA angiography examination, and then killed by air embolism. The non-necrotic region and surrounding tissues were obtained from the peripheral tumor for iNOS, HIF-1α, VEGF, Bcl-2, and Bax protein expression analysis. Protein expression of iNOS, HIF-1α, VEGF, and Bcl-2 in APR-TACE1 were significantly lower than those in groups APR-TACE2, TACE, and IVR-TACE ( p < 0.05). iNOS, HIF-1α, and VEGF in APR-TACE2 were lower than those in TACE ( p < 0.05). iNOS and VEGF in APR-TACE2 were significantly lower than those in IVR-TACE ( p < 0.05). iNOS in IVR-TACE was significantly lower than that in TACE ( p < 0.05). The expression levels of Bcl-2 and Bax were statistically significant between APR-TACE2 and TACE ( p < 0.05). The MVD of the tumor tissue in APR-TACE1 was lower than that of groups APR-TACE2, TACE, IVR-TACE with statistical difference ( p < 0.05). However, MVD of APR-TACE2 was lower than that of groups TACE, IVR-TACE with significant statistical difference ( p < 0.05). Arterial instillation of rapamycin+TACE in treatment of rabbit hepatic xenograft tumors can reduce tumor neovascularization and inhibit iNOS, HIF-1α, VEGF, Bcl-2, and Bax protein expression.

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A study on the inhibition of VEGF expression in salivary gland adenoid cystic carcinoma cells via iNOS gene RNAi in vitro

Cited by 7 publications

References 32 publications

Colocalization of VEGF and iNOS in Parenchymal and Stromal Cells of Oral Squamous Cell Carcinoma

Colocalization of VEGF and iNOS in Parenchymal and Stromal Cells of Oral Squamous Cell Carcinoma

Adenoid cystic carcinoma: current therapy and potential therapeutic advances based on genomic profiling

Arterial instillation of rapamycin in treatment of rabbit hepatic xenograft tumors and its effects on VEGF, iNOS, HIF-1α, Bcl-2, Bax expression and microvessel density

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