A sub-individual multilevel approach for an integrative assessment of CuO nanoparticle effects on Corbicula fluminea

Is micropollutant-loaded powdered activated carbon from a wastewater treatment plant toxic to the bivalve Corbicula sp.?

“…Asterisks give significant differences between the control and the treatments; *: 0.01 ≤ p ≤ 0.05; for catalase, the difference between control and initial biomarker response was also significant There was, however, a marked difference in the filtration rate between the five-and 10-week exposure although the bivalves were marked to compensate for individual variation. In the literature, high variations in the filtration rate commonly occur in experiments with Corbicula sp., e.g., ranging from 10 mL/h/bivalve [37] to 160 mL/h/bivalve [38]. Way et al [29] described a large variation in the filtration rate within their study and also in the literature.…”

“…Several research articles have shown that bivalves including Corbicula sp. react with reduced filtration rates when exposed to pollutants [53] and also to particles, such as metal nanoparticles [37] and microplastic [38] at moderate concentrations and after relatively short exposure periods of 96 h. Reduced filtration rates may be caused by neurotoxicity [38], histopathological alteration in gills [54], or avoidance of pollutant uptake due to valve closing [53]. In the present study, no such effect was observed and no difference of the exposed bivalves to the controls could be detected despite comparatively high PAC concentrations and rather long exposure periods.…”

“…is already known for its biomonitor and biofiltering abilities and shows sensitive biomarker responses when exposed to pollutants [27,33,35,36]. Apart from this, the filtration rate has been shown to be a sensible endpoint as well [37,38]. In the present study, we examined if the presence of PAC has negative effects on the filtration rate and induces biomarker responses in Corbicula sp.…”

Effects and bioaccumulation of Cr(III), Cr(VI) and their mixture in the freshwater mussel Corbicula fluminea

“…Real-time PCR was performed on a StepOnePlus PCR system using the Fast SYBR® green master mix (Applied Biosystem®, CA, USA). Amplifications were performed with 50 ng of template, or water (no template control), or 50 ng total RNA (no RT control) using 200 to 300 nM of primers in a final volume of 20 µL (Koehle-Divo et al, 2019). The cycling conditions were 20 s at 95°C, followed by 40 cycles of 10 s at 95°C (except for CuZn SOD, HSP70, PS3 and GST-pi: 3 s at 95°C) and 30 s at 60° C. A melting curve analysis (65° C-95° C) was performed to ensure the absence of artefacts and the expression of the genes of interest was normalized to that of PS3 using the 2 -ΔΔCt method.…”

“…Once inside the cell, Cr(VI) undergoes rapid reduction to Cr(III). The reduction reactions cause oxidative stress damage and produce intracellular Cr(III) that can further react with nucleic acids causing mutagenic and genotoxic effects (Kortenkamp et al, 1991;Sawicka et al, 2021;Zhitkovich, 2005). Chromate ions also show limited reactivity towards environmental particles, which usually bear a net negative charge at pH values above 4 (Warren and Haack, 2001).…”