A Substitute for the Feulgen Staining Technique

Greig, H. B. W.

doi:10.1136/jcp.12.1.93

Cited by 6 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fuelgen reaction is specific for deoxyribonucleoprotein; thus, nuclear chromatin and chromosomes are stained. Nucleoli and cytoplasm, which contain ribonucleoprotein, are feulgen negative (12).…”

Section: Discussionmentioning

confidence: 99%

Detection of nucleoproteins in squamous cell carcinoma, and dysplastic and normal mucosa in the oral cavity by methyl green-pyronin staining

et al. 2010

View full text Add to dashboard Cite

Squamous cell carcinoma (SCC) is the most malignant neoplasm of the oral cavity. The mortality rate associated with head and neck cancers remains high, and thus detection techniques are important. In the present study, methyl green-pyronin staining was applied to 5 normal mucosa specimens, 10 cases of dysplastic mucosa, 12 cases of SCC (grade I), 10 cases of SCC (grade II) and 5 cases of SCC (grade III) in the oral cavity. This is the first study in which nuclear and nucleolar cytomorphometric parameters (diameter, area and number) in such oral lesions were evaluated using this method. As methyl green-pyronin selectively stains nucleic acids, it can differentiate between DNA (green) and RNA (red). The results reveal that the proposed method can be used to evaluate changes in the nucleus and nucleolus in premalignant lesions in the oral cavity.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection of nucleoproteins in squamous cell carcinoma, and dysplastic and normal mucosa in the oral cavity by methyl green-pyronin staining

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Feulgen staining as described by Pearse (1960) and also by the less elaborate substitute technique described by Greig (1959).…”

Section: E T H O D Smentioning

confidence: 99%

A New Type of Inclusion Bodies in Lymphocytes

Hovig¹,

Jeremic²,

Stavem³

1968

Scandinavian Journal of Haematology

View full text Add to dashboard Cite

Inclusion bodies could be demonstrated in about 8 per cent of circulating lymphocytes from a female, age 70, suffering from chronic rheumatoid arthritis and leukopenia. By light microscopy the inclusions, which measured about 1 to 4 μ. in diameter, gave a reddish‐purple colour with May‐Grünwald Giemsa stain. They were faintly stained with Sudan black or PAS, but were not stained with Toluidine blue, Feulgen, or Methyl green pyronine. By electron microscopy bundles of tubules localized in cytoplasmic vacuoles or in the cytoplasmic matrix could be demonstrated. The outer diameter of each single tubule was approximately 420 Å, and the diameter of its surrounding membrane was 140 Å. The single tubule or bundles of tubules were often coiled. The mitochondria were sometimes arranged along the periphery of the inclusions. Similar inclusions were not found in other members of the patient's family, and it is suggested that the inclusions are related to the disease(s) and/or treatment of the patient.

show abstract

An unusual variant of hereditary spherocytosis

Wiley¹,

Firkin²

1970

The American Journal of Medicine

View full text Add to dashboard Cite

A Substitute for the Feulgen Staining Technique

Cited by 6 publications

References 5 publications

Detection of nucleoproteins in squamous cell carcinoma, and dysplastic and normal mucosa in the oral cavity by methyl green-pyronin staining

Detection of nucleoproteins in squamous cell carcinoma, and dysplastic and normal mucosa in the oral cavity by methyl green-pyronin staining

A New Type of Inclusion Bodies in Lymphocytes

An unusual variant of hereditary spherocytosis

Contact Info

Product

Resources

About