A Subtyping System for Nontypable Haemophilus influenzae Based on Outer-Membrane Proteins

“…A total protein extract was prepared from NTHI1479 (19). Fractions (0.02-0.06%), extracted from 2.5-7.5 ϫ 10 5 bacteria, were visualized on SDS-PAGE (15).…”

Section: Omp P2 and P6 Quantitationmentioning

confidence: 99%

Impaired Alveolar Macrophage Response to Haemophilus Antigens in Chronic Obstructive Lung Disease

Berenson

Wrona

Grove

et al. 2006

Am J Respir Crit Care Med

View full text Add to dashboard Cite

Rationale: Interactions of nontypeable Haemophilus influenzae (NTHI) with macrophages are implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). However, the immunologic mechanisms that mediate NTHI-macrophage inflammation are poorly understood. Outer membrane protein (OMP) P6 and lipooligosaccharide (LOS) of NTHI are potent immunomodulators. We theorized that alveolar macrophages in COPD possess fundamental immune defects that permit NTHI to evade host responses. Objective: To test this hypothesis, we obtained human alveolar and blood macrophages from exsmokers with COPD, exsmokers without COPD, and nonsmokers. Methods: Alveolar and blood macrophages from each donor were incubated with purified LOS and OMP P6 and with OMP P2 and the total outer membrane preparation (0.1-1 g/ml). Measurements: Supernatants (24 h) were assayed for IL-1␤, TNF-␣, IL-10, IL-12, and IL-8 by multianalyte multiplexed flow cytometry. Results: Comparative induction of COPD and non-COPD alveolar macrophages by LOS and OMP P6 revealed diminished IL-8, TNF-␣, and IL-1␤ responses of COPD alveolar macrophages (p р 0.03 for each). COPD alveolar macrophages also had diminished responses to total outer membrane (p р 0.03 for each). In contrast, COPD blood macrophages had no significant differences among donor groups in IL-8, TNF-␣, or IL-1␤ responsiveness to NTHI antigens. Diminished IL-12 responses of COPD blood macrophages to NTHI antigens, compared with nonsmokers, could not be independently dissociated from group differences in age and pack-years. Conclusions: These findings support a paradigm of defective immune responsiveness of alveolar macrophages, but not blood macrophages, in COPD.

show abstract

“…influenzaeou\cr membrane protein (OMP) prepared as described by Murphy et at. (12). A second ELISA using whole lyophilized nonserotypabie H. influenzae cells as the solid phase was used lo determine the presence of antibodies to surface proteins other than OMP.…”

Section: Quantitation Of Antibodymentioning

confidence: 99%

Inhibition of adhesion of Haemophilus influenzae to buccal cells by respiratory secretions

Taylor

Cripps²,

Clancy³

1990

Immunology & Cell Biology

View full text Add to dashboard Cite

Summary The ability of salivary secretions and sputum sol from patients with chronic bronchitis to inhibit the adhesion of non-serotypable Haemophilus influenzae to human buecal epithelial eells has been examined. Specific H. injluenzae antibodies were measured by an enzyme-linked immunosorbent assay (ELISA). Saliva and sputum levels of ami-//, influenzae immunoglobulin A (IgA) antibody were related inversely to buceal eell adherenee (r = -0 92). Absorption of saliva and sputum sol with //. injluenzae removed antibody detected by ELISA but only partially removed inhibition of attachment of bacteria. In patients with a history of recurrent bronchitis significantly more inhibitory aetivity remained after absorption of the secretory IgA (sIgA) specific antibody compared with patients who were resistant to acute episodes of infection (/'<0 05). These observations suggest that factors, other than sIgA. are present in respiratory seeretions and are capable of modulating //. /n//ufnrat'attaehment to buceal epithelial cells.

show abstract

A Subtyping System for Nontypable Haemophilus influenzae Based on Outer-Membrane Proteins

Cited by 140 publications

References 22 publications

A family of variably expressed outer-membrane proteins (Vomp) mediates adhesion and autoaggregation in Bartonella quintana

A family of variably expressed outer-membrane proteins (Vomp) mediates adhesion and autoaggregation in Bartonella quintana

Impaired Alveolar Macrophage Response to Haemophilus Antigens in Chronic Obstructive Lung Disease

Inhibition of adhesion of Haemophilus influenzae to buccal cells by respiratory secretions

Contact Info

Product

Resources

About