A subunit gIV vaccine, produced by transfected mammalian cells in culture, induces mucosal immunity against bovine herpesvirus-1 in cattle

Hurk, Sylvia van Drunen Littel‐van den; Donkersgoed, Joyce Van; Kowalski, Jacek; Hurk, J V van den; Harland, R.; Babiuk, Lorne A.; Zamb, Timothy J.

doi:10.1016/s0264-410x(94)80055-5

Cited by 68 publications

(35 citation statements)

References 23 publications

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“…This difference in virological protection suggests that the glycoprotein gD is a better vaccine candidate than gC when developing DNA vaccines, paralleling observations made with recombinant adenoviral vectors [16]. This observation also underlines the importance of vaccination-induced neutralising antibodies for reducing BoHV-1 excretion after infection [52].…”

Section: Discussionsupporting

confidence: 60%

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

et al. 2005

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-Bovine herpesvirus 1 (BoHV-1) has frequently been used as a model for testing parameters affecting DNA immunisation in large animals like cattle. However, the selection of target antigens has been poorly studied, and most of the experiments have been conducted in mice. In the present study, we demonstrated in cattle that a DNA vaccine encoding BoHV-1 glycoprotein gD induces higher neutralising antibody titres than vaccines encoding BoHV-1 gC. Additionally, we show that a DNA vaccine encoding a secreted form of gD induces a higher immune response than a vaccine encoding full-length gD. However, the enhanced immunogenicity associated with the secretion of gD could not be extended to the glycoprotein gC. The current study also describes for the first time the development and the evaluation of a DNA vaccine encoding the major tegument protein VP8. This construct, which is the first BoHV-1 plasmid vaccine candidate that is not directed against a surface glycoprotein, induced a high BoHV-1 specific cellular immunity but no humoral immune response. The calves vaccinated with the constructs encoding full-length and truncated gD showed a non-significant tenfold reduction of virus excretion after challenge. Those calves also excreted virus for significantly (p < 0.05) shorter periods (1.5 days) than the nonvaccinated controls. The other constructs encoding gC and VP8 antigens induced no virological protection as compared to controls. Altogether the DNA vaccines induced weaker immunity and protection than conventional marker vaccines tested previously, confirming the difficulty to develop efficient DNA vaccines in large species.BoHV-1 / DNA immunisation / gC / gD / VP8

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Section: Discussionsupporting

confidence: 60%

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

et al. 2005

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“…GST-CD154 was produced as an insoluble protein in lysates of DH5␣ cells and separated on a 10% polyacrylamide gel. The gel was copper stained (25) to identify GST-CD154, which was subsequently excised, homogenized, and mixed with VSA3 adjuvant (26). Rabbits were immunized twice with the GST-CD154 formulation.…”

Section: Production Of Bovine Cd154-specific Rabbit Serummentioning

confidence: 99%

Targeting with Bovine CD154 Enhances Humoral Immune Responses Induced by a DNA Vaccine in Sheep

Manoj

Griebel²,

Babiuk³

et al. 2003

The Journal of Immunology

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CD40-CD154 interactions play an important role in regulating humoral and cell-mediated immune responses. Recently, these interactions have been exploited for the development of therapeutic and preventive treatments. The objective of this study was to test the ability of bovine CD154 to target a plasmid-encoded Ag to CD40-expressing APCs. To achieve this, a plasmid coding for bovine CD154 fused to a truncated secreted form of bovine herpesvirus 1 glycoprotein D (tgD), pSLIAtgD-CD154, was constructed. The chimeric tgD-CD154 was expressed in vitro in COS-7 cells and reacted with both glycoprotein D- and CD154-specific Abs. Both tgD and tgD-CD154 were capable of binding to epithelial cells, whereas only tgD-CD154 bound to B cells. Furthermore, dual-labeling of ovine PBMCs revealed that tgD-CD154 was bound by primarily B cells. The functional integrity of the tgD-CD154 chimera was confirmed by the induction of both IL-4-dependent B cell proliferation and tgD-specific lymphoproliferative responses in vitro. Finally, sheep immunized with pSLIAtgD-CD154 developed a more rapid primary tgD-specific Ab response and a significantly stronger tgD-specific secondary response when compared with animals immunized with pSLIAtgD and control animals. Similarly, virus-neutralizing Ab titers were significantly higher after secondary immunization with pSLIAtgD-CD154. These results demonstrate that using CD154 to target plasmid-expressed Ag can significantly enhance immune responses induced by a DNA vaccine.

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“…The Cooper and 108 strains of BHV-1 were propagated in Madin-Darby bovine kidney (MDBK) cells as previously described (48). Strain 108 was used to challenge cattle, whereas strain Cooper was used in plaque titration and virus neutralization assays.…”

Section: Cells and Virusmentioning

confidence: 99%

“…A truncated version of BHV-1 gD (tgD) was constructed by terminating the protein at amino acid 355, immediately upstream of the transmembrane anchor. It was expressed in MDBK cells under regulation of the bovine heat shock 70A (hsp70) gene promoter (21) and purified as previously discussed (48). Authentic gD (45) and tgB (24) were produced, processed, and purified as described elsewhere.…”

Section: Cells and Virusmentioning

confidence: 99%

“…At present, the greatest potential for combined efficacy, safety, antigenic specificity, and protection against BHV-1 resides in subunit vaccines consisting of one or more of the viral glycoproteins (glycoprotein B [gB], gC, and gD). We have previously shown that a subunit vaccine containing a truncated, secreted form of glycoprotein D (tgD) is as efficient as the authentic full-length gD in protecting calves against BHV-1 disease (48). However, conventional adjuvants used for subunit vaccines such as Emulsigen combined with dimethyl dioctadecyl ammonium bromide (DDA/Em) not only generate a Th2-like immune response but are not metabolized and leave injection site reactions (27,53).…”

mentioning

confidence: 99%

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The Immunogenicity and Protective Efficacy of Bovine Herpesvirus 1 Glycoprotein D plus Emulsigen Are Increased by Formulation with CpG Oligodeoxynucleotides

Ioannou¹,

Griebel²,

Hecker

et al. 2002

J Virol

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119

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The immunogenicity and protective efficacy of a bovine herpesvirus 1 (BHV-1) subunit vaccine formulated with Emulsigen (Em) and a synthetic oligodeoxynucleotide containing unmethylated CpG dinucleotides (CpG ODN) was determined in cattle. A truncated, secreted version of BHV-1 glycoprotein D (tgD) formulated with Em and CpG ODN at concentrations of 25, 2.5, or 0.25 mg/dose produced a more balanced immune response, higher levels of virus neutralizing antibodies, and greater protection after BHV-1 challenge compared to tgD adjuvanted with either Em or CpG ODN alone. In contrast, tgD formulated with Em and either 25 mg of a non-CpG ODN or another immunostimulatory compound, dimethyl dioctadecyl ammonium bromide, induced similar immunity and protection compared to tgD formulated with Em alone, a finding which confirms the immunostimulatory effect of ODN to be CpG motif mediated. Our results demonstrate the ability of CpG ODN to induce a strong and balanced immune response in a target species.

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A subunit gIV vaccine, produced by transfected mammalian cells in culture, induces mucosal immunity against bovine herpesvirus-1 in cattle

Cited by 68 publications

References 23 publications

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

Targeting with Bovine CD154 Enhances Humoral Immune Responses Induced by a DNA Vaccine in Sheep

The Immunogenicity and Protective Efficacy of Bovine Herpesvirus 1 Glycoprotein D plus Emulsigen Are Increased by Formulation with CpG Oligodeoxynucleotides

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