A Sugar Transporter Takes Up both Hexose and Sucrose for Sorbitol-Modulated In Vitro Pollen Tube Growth in Apple

Li, Chunlong; Meng, Dong; Piñeros, Miguel A.; Mao, Yuxin; Dandekar, Abhaya M.; Cheng, Lailiang

doi:10.1105/tpc.19.00638

Cited by 58 publications

(61 citation statements)

References 80 publications

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“…However, an apoplasmic barrier with the surrounding style tissues (Scott et al, 1991) predicts that plasma membrane-localized sugar transporters are required to allow transport of sugars, from style tissue to apoplasm and, subsequent to this, into elongating pollen tubes (Reinders, 2016; Goetz et al, 2017). We assume that SlSWEET5b may contribute to sugar unloading from style cells into the transmitting track, where sugar uptake into growing pollen tubes is mediated by proton driven SUC/SUT and HT type transporters (Cheng et al, 2015; Shen et al, 2019; Li et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Hexose translocation mediated by SlSWEET5b is required for pollen maturation in Solanum lycopersicum

Tseng

et al. 2021

Preprint

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Pollen fertility is critical for successful fertilization and, accordingly, for crop yield. While sugar unloading affects growth and development of all types of sink organs, the molecular nature for sugar import to tomato pollen is poorly understood. However, SWEET transporters have been proposed to function in pollen development. Here, qRT-PCR revealed that SlSWEET5b was markedly expressed in flowers when compared to the remaining tomato SlSWEETs; particularly, in the stamens of maturing flower buds undergoing mitosis. Distinct accumulation of SlSWEET5b-GUS fusion proteins was present in mature flower buds, especially in anther vascular and inner cells, symplasmic isolated pollen cells and styles. The demonstration that GFP fusion proteins located to the plasma membrane support the idea that the SlSWEET5b carrier functions in apoplasmic sugar translocation during pollen maturation. Such function is in line with data from yeast complementation experiments and radiotracer uptakes, showing that SlSWEET5b operates as a low affinity hexose-specific passive facilitator, with a KM of ~36 mM. Most importantly, RNAi-mediated suppression of SlSWEET5b expression resulted in shrunken nucleus-less pollen cells, impaired germination and low seed yield. Interestingly, stamens from SlSWEET5b-silenced tomato mutants contained significantly lower amounts of sucrose and increased invertase activity, pointing to reduced carbon supply and perturbed sucrose homeostasis in this tissue. Taken together, our findings reveal an essential role of SlSWEET5b in mediating apoplasmic hexose import into phloem unloading cells and into developing pollen cells to support pollen mitosis and maturation in tomato flowers.

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Section: Discussionmentioning

confidence: 99%

Hexose translocation mediated by SlSWEET5b is required for pollen maturation in Solanum lycopersicum

Tseng

et al. 2021

Preprint

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“…Consistent with these results, the rice hk5 hk6 mutant, disrupted for two HK cytokinin receptor genes, displayed severely delayed flowering [ 236 ]. SOC1 , FD and ARR5 -like were upregulated in sweet cherry tree buds during flowering induction when the highest amount of CKs was applied [ 237 ], as they were in apple tree buds when CKs were applied [ 11 ].…”

Section: Floweringmentioning

confidence: 99%

“…Sugars growth-related metabolic activity and as signaling entities that drive a wide array of mechanisms throughout the plant life cycle [ 1 , 2 , 3 , 4 , 5 ]. Briefly, sugar signaling is intimately linked to developmental stages, hormonal signaling and environmental conditions, and thereby is an integrative part of plant growth control [ 6 , 7 , 8 , 9 , 10 , 11 ]. Plants can sense a diversity of soluble sugars such as sucrose, glucose, fructose and trehalose-6-phosphate (T6P).…”

Section: Introductionmentioning

confidence: 99%

Convergence and Divergence of Sugar and Cytokinin Signaling in Plant Development

Wang

Gourrierec

Jiao

et al. 2021

IJMS

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Plants adjust their growth and development through a sophisticated regulatory system integrating endogenous and exogenous cues. Many of them rely on intricate crosstalk between nutrients and hormones, an effective way of coupling nutritional and developmental information and ensuring plant survival. Sugars in their different forms such as sucrose, glucose, fructose and trehalose-6-P and the hormone family of cytokinins (CKs) are major regulators of the shoot and root functioning throughout the plant life cycle. While their individual roles have been extensively investigated, their combined effects have unexpectedly received little attention, resulting in many gaps in current knowledge. The present review provides an overview of the relationship between sugars and CKs signaling in the main developmental transition during the plant lifecycle, including seed development, germination, seedling establishment, root and shoot branching, leaf senescence, and flowering. These new insights highlight the diversity and the complexity of the crosstalk between sugars and CKs and raise several questions that will open onto further investigations of these regulation networks orchestrating plant growth and development.

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“…A recent study demonstrated that sugar transporter protein MdSTP13a in apple uptakes sucrose for pollen tube elongation. Sorbitol, a major photosynthate in apple, might control MdMYB39L that binds to promoter of MdSTP13a to activate its expression [ 99 ]. In addition, expression of SWEET5 in Arabidopsis was observed in germinated pollen and it might supply sugar to pollen grain vegetative cells in late pollen development [ 33 ].…”

Section: Pollen Germination and Pollen Tube Elongationmentioning

confidence: 99%

Links between Regulatory Systems of ROS and Carbohydrates in Reproductive Development

Kiyono

Katano

Suzuki

2021

Plants

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To thrive on the earth, highly sophisticated systems to finely control reproductive development have been evolved in plants. In addition, deciphering the mechanisms underlying the reproductive development has been considered as a main research avenue because it leads to the improvement of the crop yields to fulfill the huge demand of foods for the growing world population. Numerous studies revealed the significance of ROS regulatory systems and carbohydrate transports and metabolisms in the regulation of various processes of reproductive development. However, it is poorly understood how these mechanisms function together in reproductive tissues. In this review, we discuss mode of coordination and integration between ROS regulatory systems and carbohydrate transports and metabolisms underlying reproductive development based on the hitherto findings. We then propose three mechanisms as key players that integrate ROS and carbohydrate regulatory systems. These include ROS-dependent programmed cell death (PCD), mitochondrial and respiratory metabolisms as sources of ROS and energy, and functions of arabinogalactan proteins (AGPs). It is likely that these key mechanisms govern the various signals involved in the sequential events required for proper seed production.

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A Sugar Transporter Takes Up both Hexose and Sucrose for Sorbitol-Modulated In Vitro Pollen Tube Growth in Apple

Cited by 58 publications

References 80 publications

Hexose translocation mediated by SlSWEET5b is required for pollen maturation in Solanum lycopersicum

Hexose translocation mediated by SlSWEET5b is required for pollen maturation in Solanum lycopersicum

Convergence and Divergence of Sugar and Cytokinin Signaling in Plant Development

Links between Regulatory Systems of ROS and Carbohydrates in Reproductive Development

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