A supersensitive immunofluorometric assay for rat luteinizing hormone

Haavisto, Antti

doi:10.1210/en.132.4.1687

Cited by 117 publications

(94 citation statements)

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“…We used the ultra-sensitive DELFIA rat LH assay (Wallac Oy, Turku, Finland) to measure LH in rodent serum (27). Sensitivity was 0.03 ng/mL, and the intra-and interassay coefficients of variations at 33.1 ng/mL were 3.4% and 16.0%, respectively.…”

Section: Methodsmentioning

confidence: 99%

Long-term, low-dose lead exposure alters the gonadotropin-releasing hormone system in the male rat.

Sokol

Wang

Wan

et al. 2002

Environ Health Perspect

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Lead is a male reproductive toxicant. Data suggest that rats dosed with relatively high levels of lead acetate for short periods of time induced changes in the hypothalamic gonadotropin-releasing hormone (GnRH) at the molecular level, but these changes were attenuated with increased concentration of exposure. The current study evaluated whether exposure to low levels of lead acetate over longer periods of time would produce a similar pattern of adaptation to toxicity at the molecular and biologic levels. Adult 100-day-old Sprague-Dawley male rats were dosed with 0, 0.025, 0.05, 0.1, and 0.3% lead acetate in water. Animals were killed after 1, 4, 8, and 16 weeks of treatment. Luteinzing hormone (LH) and GnRH levels were measured in serum, and lead levels were quantified in whole blood. Hypothalamic GnRH mRNA levels were also quantified. We found no significant differences in serum LH and GnRH among the groups of animals treated within each time period. A significant dose-related increase of GnRH mRNA concentrations with lead dosing occurred in animals treated for 1 week. Animals treated for more than 1 week also exhibited a significant increase in GnRH mRNA, but with an attenuation of the increase at the higher concentrations of lead with increased duration of exposure. We conclude that the signals within and between the hypothalamus and pituitary gland appear to be disrupted by long-term, low-dose lead exposure.

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Section: Methodsmentioning

confidence: 99%

Long-term, low-dose lead exposure alters the gonadotropin-releasing hormone system in the male rat.

Sokol

Wang

Wan

et al. 2002

Environ Health Perspect

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“…For seed enrichment analysis, only genes with FPKM above 1.0 were considered. For cumulative distribution function calculations, log 2 fold-change values were corrected for 3′ UTR length biases (55).…”

Section: Author Contributionsmentioning

confidence: 99%

“…For intratesticular testosterone analysis, lipid extraction was performed using 2× diethylether. Serum LH and FSH concentrations were measured using immunofluorometric assays as described previously (55). For measuring intratesticular and serum testosterone, both sera and tissue homogenates were extracted twice with 2 ml of diethyl ether, followed by conventional RIA (56).…”

Section: Author Contributionsmentioning

confidence: 99%

Loss of microRNA-7a2 induces hypogonadotropic hypogonadism and infertility

Ahmed¹,

LaPierre²,

Gasser³

et al. 2017

Journal of Clinical Investigation

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MicroRNAs (miRNAs) are negative modulators of gene expression that fine-tune numerous biological processes. miRNA loss-of-function rarely results in highly penetrant phenotypes, but rather, influences cellular responses to physiologic and pathophysiologic stresses. Here, we have reported that a single member of the evolutionarily conserved miR-7 family, miR-7a2, is essential for normal pituitary development and hypothalamic-pituitary-gonadal (HPG) function in adulthood. Genetic deletion of mir-7a2 causes infertility, with low levels of gonadotropic and sex steroid hormones, small testes or ovaries, impaired spermatogenesis, and lack of ovulation in male and female mice, respectively. We found that miR-7a2 is highly expressed in the pituitary, where it suppresses golgi glycoprotein 1 (GLG1) expression and downstream bone morphogenetic protein 4 (BMP4) signaling and also reduces expression of the prostaglandin F2a receptor negative regulator (PTGFRN), an inhibitor of prostaglandin signaling and follicle-stimulating hormone (FSH) and luteinizing hormone (LH) secretion. Our results reveal that miR-7a2 critically regulates sexual maturation and reproductive function by interconnecting miR-7 genomic circuits that regulate FSH and LH synthesis and secretion through their effects on pituitary prostaglandin and BMP4 signaling

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“…Serum levels of LH were measured by immunofluorometric assay (Delfia; PerkinElmer-Wallac, Turku, Finland) as described previously (Haavisto et al, 1993). The approximate assay sensitivity for LH was 0.0075 mg/l.…”

Section: Hormone Measurementmentioning

confidence: 99%

Transgenic GATA-4 expression induces adrenocortical tumorigenesis in C57Bl/6 mice

Chruściel

Vuorenoja

Mohanty

et al. 2013

Journal of Cell Science

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SummaryA link between elevated luteinizing hormone (LH) levels, GATA-4 and LH receptor (LHCGR) expression and gonadotropin-dependent adrenocortical tumorigenesis in humans and mice has been shown. To assess the mechanistic tumorigenic interrelationships between these factors, we transgenically expressed Gata4 under the 21-hydroxylase promoter (Cyp21a1, 21-OH) in C57Bl/6N mice. There was a gradual age-dependent increase of GATA-4 expression only in 21-OH-GATA-4 (TG) female adrenals, in association with slowly progressing neoplasia of non-steroidogenic spindle-shaped A cells in the subcapsular cortex. Gonadectomy (GDX), apparently through direct action of elevated serum LH, markedly enhanced the adrenocortical neoplasia, which now also appeared in GDX TG males. The neoplastic areas of the post-GDX TG adrenals contained, besides A cells, larger lipid-laden, steroidogenically active and LHCGRpositive B cells. Prolonged (.10 months) exposure to elevated post-GDX LH levels resulted in formation of adrenocortical adenomas in the TG mice. Intact and GDX TG mouse adrenals displayed elevated FOG-2 and decreased GATA-6 expression. Additionally, increased expression/activation of components of the Inhbb-Acvr2a-Acvr1c-Smad2/3 signaling system was observed in 12-month-old GDX TG adrenals. Our findings show that two distinct GATA-4-dependent populations of neoplastic adrenocortical cells form: non-steroidogenic LH-independent A cells and steroidogenic LH-dependent B cells.

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A supersensitive immunofluorometric assay for rat luteinizing hormone

Cited by 117 publications

References 0 publications

Long-term, low-dose lead exposure alters the gonadotropin-releasing hormone system in the male rat.

Long-term, low-dose lead exposure alters the gonadotropin-releasing hormone system in the male rat.

Loss of microRNA-7a2 induces hypogonadotropic hypogonadism and infertility

Transgenic GATA-4 expression induces adrenocortical tumorigenesis in C57Bl/6 mice

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