A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

Abstract

This research aimed to evaluate the effect of inclusion unsaturated fatty acids (UFAs) in the buffalo diet, on the sperm quality after thawing. In parallel, it was verified the degree of association among techniques to assess plasma membrane integrity (PMI) of spermatozoa. Patterns of sperm motility (subjective and computer-assisted analysis), kinetics, morphology and plasma membrane integrity were evaluated. The hypo-osmotic swelling test, nigrosin-eosin staining (E-N) and fluorescent probes propidium iodide/Hoechst 33342 (IP-H342) were adopted to evaluate PMI. The study was planned considering two experimental groups, which were evaluated in repeated measures design. over time. Eleven Murrah buffalo bulls (3.5±1.0 years, and 536.9±73.0 kg) were divided in Control Group (CONT, n=5) and Palm Oil Group (PO, n=6). Animals were fed with forage and concentrated in a controlled manner. Animals of PO received dietary supplementation containing palm oil (2% of dry matter). Dietary consumption was daily monitored and the intake level of UFAs in PO was 55.4% higher than CONT. Semen samples were collected and evaluated every 14 days. The experiment lasted 120 days, considering four periods of 30 days each (Periods I, II, III and IV) and repeated measures structure was used in statistical analysis. Significance level adopted was 5%. There was no effect of treatment on sperm motility (P>0.05), but palm oil supplementation presented a positive effect on spermatic ability to progress (derived from BCF, STR and LIN) in Period IV (P<0.05). High correlation (91%) was observed between IP-H342 and E-N techniques (Y = 0.9155 x + 2.9843, R 2 = 0.82, P≤0.05). Thus, supplementation with UFAs increased the progressivity of spermatozoa after 90 days, and had no effect on other seminal parameters. Eosin-nigrosin stain can be used efficiently as a predictor of the level of PMI when conditions for implementing fluorescent probes not present.

Increased quality of in natura and cryopreserved semen of water buffaloes supplemented with saturated and unsaturated fatty acids from the palm oil industry

“…In addition, lower quality of bovine cryopreserved semen has been reported as a consequence of lower C18:2 and C20:4 concentrations in spermatozoa ( Argov et al, 2007 ). The difference in C18:2 abundance in the biological matrices analyzed in the present study associate with previous results obtained by our research group in complementary experiments with water buffaloes ( Gonçalves et al, 2014 ; Santos et al, 2017 ) allow inferring that seminal plasma composition varies more immediately than sperm membrane composition due to the lipemic effect noted when animals are fed diets rich in fats ( Silva et al, 2014 ).…”

“…Although C18:3 was detected in the plasma membrane of spermatozoa from bulls supplemented with crude palm oil, their derived metabolites were not detected. Dietary supplementation with unsaturated fatty acids reportedly increases the quality of cryopreserved buffalo semen ( Gonçalves et al, 2014 ) likely because they modulate the structure and lipid composition of cell membranes ( Fan et al, 2003 ; Calder, 2012 ). However, in the present study, the most significant changes in sperm membrane composition were effectively related to the differences in expression of saturated fatty acids.…”

“…Recent researches on the adoption of vegetable oils or fats as alternative energy sources for ruminants have been carried out ( Esmaeili et al, 2014 ; Gürler et al, 2015 ; Moallem et al, 2015 ; Khoshvaght et al, 2016 ), which has brought attention to the inclusion of agro-industry products in ruminant feed ( Van Tran et al, 2017 ). Among such products are by-products from oil palm ( Elaeis guineensis ) fruit processing ( Santos et al, 2014 ; Gonçalves et al, 2014 ; Silva et al, 2014 ), which are available in many parts of the world. During industrialization, palm oil is extracted from the pulp and its physical refining generates a compound known as palm oil deodorizer distillate.…”

Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm

“…These results indicate that perhaps the constituents of the TES-TRIS diluent evaluated in our experiment were more efficient in interacting with and protecting the integrity of sperm membranes, which is an essential function to increase the fertility indices of cryopreserved semen. For this reason, perhaps the TES-TRIS diluent has been used since the 1980s and is still used efficiently by numerous researchers for freezing buffalo semen (OBA et al, 1993;SILVA et al, 2002;MOOSE et al, 2007;PESSOA et al, 2011;BRITO, 2014;GONÇALVES et al, 2014;REIS, 2015).…”

Avaliação do efeito da piplartina e cinamidas em cultivos de Leishmania amazonensis, Plasmodium falciparum e de células peritoneais de camundongos swiss

“…Para o ensaio utilizando a coloração por nigrosina, a leitura dos resultados é realizada por microscopia óptica com contagem manual em câmara de Neubauer consumindo muito tempo do pesquisador e limitando a quantidade de amostras a serem lidas de uma única vez (Coelho, 2008;Souza et al, 2015). Mesmo assim, pode ser considerada uma técnica simples e de baixo-custo (Gonçalves et al, 2014).…”