A supramolecular hydrogel based on the combination of YIGSR and RGD enhances mesenchymal stem cells paracrine function via integrin α2β1 and PI3K/AKT signaling pathway for acute kidney injury therapy

Han, Qiuxia; Ai, Sifan; Hong, Quan; Zhang, Chuyue; Song, Youjian; Wang, Xiaochen; Wang, Xu; Cui, Shaoyuan; Li, Zongjin; Zhu, Hanyu; Yang, Zhimou; Chen, Xiangmei; Cai, Guangyan

doi:10.1016/j.cej.2022.135088

Cited by 22 publications

(9 citation statements)

References 39 publications

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“…For example, Tat (trans-activator) protein-derived peptide named R-peptide (sequence: GRKKRRQRRRGGGRGD) can maintain the MSCs properties (e.g., high proliferation and differentiation capacity), and E7 peptide (sequence: EPLQLKM) can improve adhesion and stem cell phenotype maintenance . A novel peptide (sequence: YIGSRGD) consisting of YIGSR and RGD peptides can augment the paracrine function of the MSCs, thereby enhancing their antiapoptotic and anti-inflammatory effects . These peptides can be immobilized on the carrier via chemical cross-linking, creating biomimetic interfaces to facilitate cell functions.…”

Section: Resultsmentioning

confidence: 99%

“…62 A novel peptide (sequence: YIG-SRGD) consisting of YIGSR and RGD peptides can augment the paracrine function of the MSCs, thereby enhancing their antiapoptotic and anti-inflammatory effects. 63 These peptides can be immobilized on the carrier via chemical cross-linking, creating biomimetic interfaces to facilitate cell functions. In summary, the PET-Gel-S carrier had good cytocompatibility and promoted the adhesion and proliferation of the HUCMSCs.…”

Section: Characterization Of Pet Sponge After Thermalmentioning

confidence: 99%

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Autoclavable Polydopamine-Gelatin-Modified Polyethylene Terephthalate Microfibrous Carriers Regulate the Proliferation and Paracrine Signaling of Mesenchymal Stem Cells

et al. 2022

ACS Appl. Polym. Mater.

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Mesenchymal stem cells (MSCs) have attracted considerable attention in cell therapies and tissue engineering for their multilineage differentiation potential and immunomodulatory properties. However, MSCs gradually lose their stemness and multipotentiality in prolonged two-dimensional (2D) culture in vitro. Herein, a three-dimensional (3D) polyethylene terephthalate (PET) microfiber sponge served as the substrate, and gelatin macromolecules were grafted onto PET through the adsorption of polydopamine to prepare a 3D cell carrier (PET-Gel) with an interpenetrating network structure. The cell carrier could be sterilized by autoclaving. The material characterization results showed that the 3D carrier had good coating stability, thermal stability, and mechanical properties; these properties did not decrease after autoclaving. In vitro cell experiments revealed that the 3D cell carrier promoted the adhesion and proliferation of human umbilical cord MSCs (HUCMSCs). In long-term culture (2 weeks), the cell proliferation rate was 2.4-fold higher than that on 2D plates because the interpenetrating network structure of the carrier led to a larger growing area than 2D plates and the cells infiltrated into the interior of the carrier. Simultaneously, HUCMSCs on PET-Gel expressed surface-specific antigens of the MSCs. The typical paracrine cytokine expression was characterized using qPCR and Western blotting. The paracrine function of cells on the 3D carrier was nearly equivalent to that on the 2D plates. These results showed that the PET-Gel carrier was suitable for the long-term culture of HUCMSCs in vitro.

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Section: Resultsmentioning

confidence: 99%

Section: Characterization Of Pet Sponge After Thermalmentioning

confidence: 99%

Autoclavable Polydopamine-Gelatin-Modified Polyethylene Terephthalate Microfibrous Carriers Regulate the Proliferation and Paracrine Signaling of Mesenchymal Stem Cells

et al. 2022

ACS Appl. Polym. Mater.

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“…3D). SF or SF-based biomaterials has been reported to regulate cell behaviors by PI3K-Akt signaling pathway, and importantly this pathway is also found to mediate the collagen synthesis in mice and the angiogenic factor secretion in MSCs ( 18, 54–56 ). It well known that cell-matrix adhesion and interaction play an essentials role in various important biological processes, such as cell adhesion, cell proliferation, cell migration and regulation of gene expression ( 57 ).…”

Section: Resultsmentioning

confidence: 99%

“…3E). Interestingly, PI3K-AKT signaling is a downstream pathway of integrin signaling, and previous studies reported that the paracrine functions of MSCs could be enhanced by activating integrin/PI3K/AKT signaling pathways ( 56 ). Collectively, these findings highly suggested that SF triggered various cellular responses of MSCs by activating integrin signaling pathway.…”

Section: Resultsmentioning

confidence: 99%

Multiomics reveal that silk fibroin and sericin differentially potentiate the paracrine functions of mesenchymal stem cells and enhance tissue regeneration

Zhang

Sheng

Chen

et al. 2022

Preprint

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Silk fibroin (SF) and sericin (SS), the two major proteins of silk, are attractive biomaterials that show great potential in regenerative medicine. However, their biochemical interactions with stem cells were not fully understood. Here, we employed multiomics to obtain a global view of the triggered cellular processes and pathways of MSCs by SF and SS. Integrated RNA-seq and proteomics revealed that SF and SS strongly enhanced the paracrine activity of MSCs through differentially activating integrin and glycolytic pathways, rather than directly regulating stem cell fate to initiate multiple but distinct biological processes in MSCs. Those specific paracrine signals of MSCs stimulated by SF and SS effectively promoted skin wound healing by influencing the behaviors of multiple resident cells in skin wound microenvironments. This study provides comprehensive and reliable insights into the cellular interactions with SF and SS, enabling future development of silk-based therapeutics for tissue engineering and stem cell therapy.

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“…Studies related to the detection and function of ligand peptide sequences in ECM components have enhanced peptide science in tissue engineering strategies [ 224 , 227 , 229 , 230 ]. Based on this information, tissue engineering strategies have involved the selection of suitable ECM components with specific CAPS for hydrogel synthesis [ 231 ], isolation of certain peptides from predesignated sources, or fabrication of peptides using peptide synthesizers (Biotage®, CEM®, LABX®) and subsequent hydrogel/scaffold modification are prevalent approaches for CAPs utilization in tissue engineering strategies [ 161 , 232 ]. From the molecular structure, sequences such as DEGA, IKVAV, and PHSRN with cell adherence characteristics are present in the structure of collagen [ 233 ], laminin [ 234 ], and fibronectin [ 235 ], respectively.…”

Section: Chemical Cues In Skeletal Muscle Regenerationmentioning

confidence: 99%

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

et al. 2023

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Muscular diseases and injuries are challenging issues in human medicine, resulting in physical disability. The advent of tissue engineering approaches has paved the way for the restoration and regeneration of injured muscle tissues along with available conventional therapies. Despite recent advances in the fabrication, synthesis, and application of hydrogels in terms of muscle tissue, there is a long way to find appropriate hydrogel types in patients with congenital and/or acquired musculoskeletal injuries. Regarding specific muscular tissue microenvironments, the applied hydrogels should provide a suitable platform for the activation of endogenous reparative mechanisms and concurrently deliver transplanting cells and therapeutics into the injured sites. Here, we aimed to highlight recent advances in muscle tissue engineering with a focus on recent strategies related to the regulation of vascularization and immune system response at the site of injury.

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A supramolecular hydrogel based on the combination of YIGSR and RGD enhances mesenchymal stem cells paracrine function via integrin α2β1 and PI3K/AKT signaling pathway for acute kidney injury therapy

Cited by 22 publications

References 39 publications

Autoclavable Polydopamine-Gelatin-Modified Polyethylene Terephthalate Microfibrous Carriers Regulate the Proliferation and Paracrine Signaling of Mesenchymal Stem Cells

Autoclavable Polydopamine-Gelatin-Modified Polyethylene Terephthalate Microfibrous Carriers Regulate the Proliferation and Paracrine Signaling of Mesenchymal Stem Cells

Multiomics reveal that silk fibroin and sericin differentially potentiate the paracrine functions of mesenchymal stem cells and enhance tissue regeneration

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

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