A survey for piroplasmids in horses and Bactrian camels in North-Eastern Mongolia

Sloboda, Michal; Jirků, Milan; Lukešová, D.; Qablan, Moneeb A.; Batsukh, Zayat; Fiala, Ivan; Hořín, Petr; Modrý, David; Lukeš, Julius

doi:10.1016/j.vetpar.2011.01.064

Cited by 35 publications

(25 citation statements)

References 23 publications

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“…Such finding is in agreement with previous studies from South Africa (Bhoora et al, 2009), Greece (Kouam et al, 2010), Mongolia (Sloboda et al, 2011) and Jordan (Qablan et al, 2013).…”

Section: Discussionsupporting

confidence: 94%

“…Few studies, based on molecular detection, found the age a significant factor influencing the prevalence of equine piroplasmids (Kouam et al, 2010;Sloboda et al, 2011;Qablan et al, 2013). The same effect of age is apparent also among our dataset, as T. equi infection was more prevalent among equids aged 4 years and older.…”

Section: Discussionsupporting

confidence: 81%

“…Genomic DNA was isolated from blood samples (using 200 l preserved in EDTA or same amount preserved in ethanol) by modified phenol-chloroform method. For equine piroplasmids detection, two PCR assays ("catch-all" and multiplex) were followed (for further details see Sloboda et al, 2011). Detection of canine piroplasmids was commenced with nested PCR (Jefferies et al, 2007).…”

Section: Microscopy Dna Extraction and Pcrmentioning

confidence: 99%

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Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir

Gallusová

Qablan

D’Amico

et al. 2014

Veterinary Parasitology

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“…Such finding is in agreement with previous studies from South Africa (Bhoora et al, 2009), Greece (Kouam et al, 2010), Mongolia (Sloboda et al, 2011) and Jordan (Qablan et al, 2013).…”

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 81%

Section: Microscopy Dna Extraction and Pcrmentioning

confidence: 99%

See 1 more Smart Citation

Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir

Gallusová

Qablan

D’Amico

et al. 2014

Veterinary Parasitology

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“…Typically, canine piroplasmosis is caused by B. canis and B. gibsoni. In our study, we used universal primers targeting the 18S rRNA gene for detection of piroplasmids (Sloboda et al 2011) and confirmed the presence of piroplasms in the blood of three examined dogs. Interestingly, sequencing of the amplicons did not reveal the above-mentioned known canine hostspecific species; indisputably, the obtained sequences are most similar to those of equine piroplasmids B. caballi and T. equi.…”

Section: Discussionsupporting

confidence: 59%

“…The 25-μl PCR reaction consisted of 2 μl of template DNA (25 ng), 12.5 μl of Combi PPP Master Mix (Top-Bio s.r.o., Prague, Czech Republic), 10 μM of each of the primers and 8.5 μl of PCR water. Amplification was carried out under conditions given by Inokuma et al (2002), Walls et al (2000, and Sloboda et al (2011), for A. phagocytophilum, H. canis, and piroplasmids, respectively.…”

Section: Ticksmentioning

confidence: 99%

Stray dogs of northern Jordan as reservoirs of ticks and tick-borne hemopathogens

et al. 2012

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Some aspects on tick species in Mongolia and their potential role in the transmission of equine piroplasms, Anaplasma phagocytophilum and Borrelia burgdorferi L.

et al. 2018

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Ticks are cosmopolitan vectors of numerous diseases, and detection of various pathogens in ticks can help to assess their distribution. In the current study, 528 adult ticks were collected from grazing animals or the ground in ten different Mongolian provinces. Dermacentor nuttalli constituted 76.1% of them and was found in all ecozones except the eastern desert. Dermacentor marginatus (8.3%), Dermacentor silvarum (1.1%) and Ixodes persulcatus (3.0%) were found in the northern forest areas and Hyalomma asiaticum (11.4%) only in the southern (semi-)desert. Of these, 359 ticks were subjected to DNA extraction and PCR was carried out to detect various pathogens. Anaplasma spp. was found in D. marginatus and D. nuttalli (2.5% positive each), including flagged specimen and identified as Anaplasma phagocytophilum. Borrelia spp. were found in 2.5% of the ticks (mostly in I. persulcatus) and identified as Borrelia garinii. Babesia spp. (40%) identified as Babesia caballi were detected in all five tick species including flagged Dermacentor spp. and I. persulcatus, and 3.5% of the ticks (all species except D. silvarum) were positive for Theileria spp. identified as Theileria equi. The piroplasms were found in all provinces. Tick-borne encephalitis virus was not detected. The results highlight the high risk of equine piroplasmosis in Mongolia, which is a concern for both the nomadic population who rely on horses for transport and for conservation of Przewalski's horses in Mongolia. In addition, zoonotic agents such as the avian B. garinii and A. phagocytophilum were also detected, outlining a high risk for exposed humans.

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A survey for piroplasmids in horses and Bactrian camels in North-Eastern Mongolia

Cited by 35 publications

References 23 publications

Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir

Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir

Stray dogs of northern Jordan as reservoirs of ticks and tick-borne hemopathogens

Some aspects on tick species in Mongolia and their potential role in the transmission of equine piroplasms, Anaplasma phagocytophilum and Borrelia burgdorferi L.

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