A survey of Blastocystis sp. in livestock, pets, and zoo animals in Japan

Abe, Niichiro; Nagoshi, Mizuho; Takami, Kazutoshi; Sawano, Yoshinori; Yoshikawa, Hideki

doi:10.1016/s0304-4017(02)00050-x

Cited by 116 publications

(89 citation statements)

References 30 publications

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“…Our findings are in agreement with a similar study conducted in Japan (Abe et al 2002) and Bangkok ( Inpankaew et al 2007). It should be noted that only the studies indicating exclusively the absence oiBlastocystis are included, as various parasitological studies on dog faeces do not refer Blastocystis as a finding, but is is not clear if the parasite was ignored, or it was not detected.…”

Section: Discussionsupporting

confidence: 83%

Molecular screening for Blastocystìs sp. in canine faecal samples in Greece

Spanakos

Papadogiannakis

Kontos

et al. 2017

J Hellenic Vet Med Soc

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• Μοριακή αναζήτηση της Blastocystìs sp. σε κόπρανα σκύλου στην Ελλάδα. ABSTRACT.Blastocystìs is an anaerobic parasitic microorganism which has been found in the intestinal tract of many vertebrates including humans and dogs. Recently, members of Blastocystìs sp. were classified into nine sub-types, based on phylogenetic trees derived from sequence analysis of the small sub-unit ribosomal RNA gene. The pathogenesis of Blastocystìs in the dog remains uncertain and the existence of pathogenic and non-pathogenic sub-types is under investigation. Feacal samples from 30 privately owned and 42 stray dogs, originated from central and northern Greece, were examined by both light microscopy and PCR assays for the detection of Blastocystìs sp. All samples were found to be negative by both assays. It seems that, in Greece, the dog is unlikely to be a "reservoir" of Blastocystìs sp., although a large scale epidemiologic study is needed to be carried out in order for more conclusive results to be drawn.Keywords: Blastocystìs, dog, light microscopy, PCR, Greece ΠΕΡΙΑΗΨΗ. Η Blastocystìs είναι ένα αναερόβιο παράσιτο που έχει βρεθεί στο έντερο πολλών σπονδυλωτών, περιλαμβανομένων του ανθρώπου και του σκύλου. Πρόσφατα, η Blastocystìs ταξινομήθηκε σε εννέα υπότυπους, βάσει των φυλογενετικών δένδρων που προέκυψαν από την ανάλυση της αλληλουχίας της μικρής υποομάδας του rRNA γονιδίου. Η παθογένεια της Blastocystìs στο σκύλο παραμένει ασαφής και η ύπαρξη παθογόνων και μη-παθογόνων υπότυπων ακόμη ερευνάται. Δείγματα κοπράνων από 30 δεσποζόμενους και 42 αδέσποτους σκύλους, προερχόμενους από την Κεντρική και Βόρεια Ελλάδα εξετάστηκαν μικρο σκοπικά και με τη μέθοδο PCR για τυχόν ανίχνευση της Blastocystìs sp. Όλα τα δείγματα βρέθηκαν αρνητικά και με τις δύο μεθόδους. Φαίνεται ότι στην Ελλάδα ο σκύλος μάλλον δεν συνιστά «δεξαμενή» της Blastocystìs sp., αν και απαιτείται επιδημιολογική μελέτη μεγάλης κλίμακας για να εξαχθούν ασφαλή συμπεράσματα.

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Section: Discussionsupporting

confidence: 83%

Molecular screening for Blastocystìs sp. in canine faecal samples in Greece

Spanakos

Papadogiannakis

Kontos

et al. 2017

J Hellenic Vet Med Soc

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“…Zoonotic transmission of B. hominis has been speculated since epidemiological studies suggested a linkage between close contact with animals and blastocystosis in humans (8,17). In addition, Blastocystislike organisms were detected in a wide range of animals (1,4,9,16). However, there was no conclusive evidence demonstrating animal-to-human transmission and zoonotic potential of B. hominis.…”

mentioning

confidence: 99%

Blastocystis Isolates from a Pig and a Horse Are Closely Related to Blastocystis hominis

Thathaisong¹,

Worapong

Mungthin³

et al. 2003

J Clin Microbiol

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Blastocystis has a widespread distribution in a variety of animals, which is a potential source of infection for humans. However, the contribution of zoonotic transmission remains unclear due to the absence of molecular proof of these organisms being identical to those found in humans. We report herein the similar subgroup of Blastocystis isolates from humans, pigs, and a horse using a restriction fragment length polymorphism (RFLP) analysis of partial small-subunit ribosomal DNA (ssu rDNA). Additionally, sequence and phylogenic analysis of partial ssu rDNA of Blastocystis from a human, a pig, and a horse sharing a common subgroup shows that Blastocystis isolates from a pig and a horse were monophyletic and closely related to B. hominis, with 92 to 94% identity. These results suggest the possibility of zoonotic potential of Blastocystis.Blastocystis hominis is one of the most common intestinal protozoa found in humans. This organism has been recognized as a causative agent of diarrhea both in immunocompromised and immunocompetent hosts in several studies (6,8). However, its role in human disease is still intensely debated since most cases are asymptomatic (2,18,21,23). It has been reported from both developed and developing countries, with a high prevalence in tropical areas, ranging between 30 and 50% (2, 15, 21). Our knowledge of this organism in several aspects, including its transmission, is still unclear. Zoonotic transmission of B. hominis has been speculated since epidemiological studies suggested a linkage between close contact with animals and blastocystosis in humans (8,17). In addition, Blastocystislike organisms were detected in a wide range of animals (1, 4, 9, 16). However, there was no conclusive evidence demonstrating animal-to-human transmission and zoonotic potential of B. hominis. One of the difficulties is the identification of these organisms since infection with Blastocystis has been diagnosed on the basis of morphology, host of origin, and in vitro culture characteristics (4). Although the morphology of Blastocystis detected in some animals was similar to those found in humans, only few animal isolates of Blastocystis were genetically proven to be identical to human isolates (7,25). Using a restriction fragment length polymorphism (RFLP) analysis of small-subunit ribosomal DNA (ssu rDNA), Clark identified a ribodeme of Blastocystis isolated from guinea pigs similar to that found in B. hominis (7). Yoshikawa et al. also showed that the pattern of random amplified polymorphic DNA in Blastocystis isolated from a chicken was similar to that displayed by B. hominis isolate HE87-1 (25). These observations, however, were limited and lack epidemiological data to support the association. Therefore, to determine the zoonotic potential of B. hominis, we conducted a study of genotypic characterization using RFLP analysis of partial ssu rDNAs of Blastocystis isolated from humans compared to those isolated from animals. ssu rDNAs of Blastocystis isolated from a human, a pig, and a horse were also partia...

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“…Blastocystis infection causes diarrhoea, bloating of the stomach and other gastrointestinal symptoms with recent studies showing the existence of pathogenic and nonpathogenic "strains" (Tan et al 2008). The organism has been shown to be present in a wide range of both captive and farm animals including birds (Boreham & Stenzel 1993, Abe et al 2002. The prevalence of infection in animal workers is higher (44%) than in the normal population (17%) (Suresh et al 2001), suggesting that close proximity with animals may facilitate transmission (Rajah Salim et al 1999).…”

Section: Ranking By Importancementioning

confidence: 99%

Animal Waste, Water Quality and Human Health

Dufour¹,

Bartram²,

Bos³

et al. 2013

wio

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The excreta (faeces and urine) of mammals and birds are widespread across planet Earth and frequently contaminate water that is used for bathing and recreation, that is treated and distributed for human consumption, and that is used to irrigate crops.The risk that such contamination represents to human health is inadequately understood. It is widely assumed that faeces of animals represents a lesser risk to human health than human faeces of because of the 'species barrier' and especially the species-specificity of most viruses. This book points to a rational and systematic way of assessing the risks and to cost-effective approaches to manage them. The information presented is expected to have important consequences for prioritisation of preventive and remedial interventions.

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A survey of Blastocystis sp. in livestock, pets, and zoo animals in Japan

Cited by 116 publications

References 30 publications

Molecular screening for Blastocystìs sp. in canine faecal samples in Greece

Molecular screening for Blastocystìs sp. in canine faecal samples in Greece

Blastocystis Isolates from a Pig and a Horse Are Closely Related to Blastocystis hominis

Animal Waste, Water Quality and Human Health

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