A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles

Fernández-Penas, Raquel; Verdugo‐Escamilla, Cristóbal; Triunfo, Carla; Gärtner, Stefanie; D’Urso, Annarita; Oltolina, Francesca; Follenzi, Antonia; Maoloni, Gabriele; Cölfen, Helmut; Falini, Giuseppe; Gómez‐Morales, Jaime

doi:10.1039/d3tb00856h

Cited by 6 publications

(2 citation statements)

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“…Most of the previous methods to obtain Ap particles from ground ES consisted of two steps, the first one being calcination (>800–900 °C), thus completely destroying the ES organic matrix. Herein, we applied the “one-pot” hydrothermal method [ 27 ]. The process can be classified as a mineral replacement reaction in which a Ca-salt (e.g., calcium carbonate) is replaced with another one having a lower solubility product (e.g., calcium phosphate apatite).…”

Section: Discussionmentioning

confidence: 99%

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Hydrothermal Transformation of Eggshell Calcium Carbonate into Apatite Micro-Nanoparticles: Cytocompatibility and Osteoinductive Properties

et al. 2023

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The eggshell is a biomineral consisting of CaCO3 in the form of calcite phase and a pervading organic matrix (1–3.5 wt.%). Transforming eggshell calcite particles into calcium phosphate (apatite) micro-nanoparticles opens the door to repurposing the eggshell waste as materials with potential biomedical applications, fulfilling the principles of the circular economy. Previous methods to obtain these particles consisted mainly of two steps, the first one involving the calcination of the eggshell. In this research, direct transformation by a one-pot hydrothermal method ranging from 100–200 °C was studied, using suspensions with a stoichiometric P/CaCO3 ratio, K2HPO4 as P reagent, and eggshells particles (Ø < 50 μm) both untreated and treated with NaClO to remove surface organic matter. In the untreated group, the complete conversion was achieved at 160 °C, and most particles displayed a hexagonal plate morphology, eventually with a central hole. In the treated group, this replacement occurred at 180 °C, yielding granular (spherulitic) apatite nanoparticles. The eggshell particles and apatite micro-nanoparticles were cytocompatible when incubated with MG-63 human osteosarcoma cells and m17.ASC murine mesenchymal stem cells and promoted the osteogenic differentiation of m17.ASC cells. The study results are useful for designing and fabricating biocompatible microstructured materials with osteoinductive properties for applications in bone tissue engineering and dentistry.

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Section: Discussionmentioning

confidence: 99%

“…Recently, a “one-pot” method to directly transform biogenic CaCO 3 particles into apatite micro-nanoparticles under mild hydrothermal conditions was set up [ 27 ]. The method avoids the calcination step as well as the use of acids and other additives.…”

Section: Introductionmentioning

confidence: 99%

Hydrothermal Transformation of Eggshell Calcium Carbonate into Apatite Micro-Nanoparticles: Cytocompatibility and Osteoinductive Properties

et al. 2023

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Mechanical performance of seashell-reinforced polymer composites for structural applications

Nurazzi,

Norrrahim,

Mulla

et al. 2024

Polymer Composites Derived From Animal Sources

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Waste-to-resource: Extraction and transformation of aquatic biomaterials for regenerative medicine

Cao,

Zeng,

Yuan

et al. 2025

Biomaterials Advances

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A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles

Abstract: A straightforward, one-pot, low-temperature hydrothermal method has been set up to transform calcium carbonate particles from oyster shell waste into hydroxyapatite micro/nanoparticles with osteoinductive properties.

Cited by 6 publications

References 75 publications

Hydrothermal Transformation of Eggshell Calcium Carbonate into Apatite Micro-Nanoparticles: Cytocompatibility and Osteoinductive Properties

Hydrothermal Transformation of Eggshell Calcium Carbonate into Apatite Micro-Nanoparticles: Cytocompatibility and Osteoinductive Properties

Mechanical performance of seashell-reinforced polymer composites for structural applications

Waste-to-resource: Extraction and transformation of aquatic biomaterials for regenerative medicine

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