A switch in pdgfrb cell-derived ECM composition prevents inhibitory scarring and promotes axon regeneration in the zebrafish spinal cord

Tsata, Vasiliki; Möllmert, Stephanie; Schweitzer, Christine; Kolb, Julia; Möckel, Conrad; Böhm, Benjamin; Rosso, Gonzalo; Lange, Christian; Lesche, Mathias; Hammer, Juliane; Kesavan, Gokul; Beis, Dimitris; Guck, Jochen; Brand, Michael; Wehner, Daniel

doi:10.1016/j.devcel.2020.12.009

Cited by 59 publications

(79 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, transgenic zebrafish reporters for visualizing all types of mural cells have not been available to investigators, hampering our understanding of the complex processes underlying mural cell development and subsequent vascular maturation steps. It is noteworthy, however, that several mural cell reporter lines have been developed in the last decade in our and other labs ( Table 1 ), allowing us to achieve a better understanding of mural cell development and functions [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Zebrafish Vascular Mural Cell Biology: Recent Advances, Development, and Functions

Ando

Ishii

Fukuhara

2021

Life

View full text Add to dashboard Cite

Recruitment of mural cells to the vascular wall is essential for forming the vasculature as well as maintaining proper vascular functions. In recent years, zebrafish genetic tools for mural cell biology have improved substantially. Fluorescently labeled zebrafish mural cell reporter lines enable us to study, with higher spatiotemporal resolution than ever, the processes of mural cell development from their progenitors. Furthermore, recent phenotypic analysis of platelet-derived growth factor beta mutant zebrafish revealed well-conserved organotypic mural cell development and functions in vertebrates with the unique features of zebrafish. However, comprehensive reviews of zebrafish mural cells are lacking. Therefore, herein, we highlight recent advances in zebrafish mural cell tools. We also summarize the fundamental features of zebrafish mural cell development, especially at early stages, and functions.

show abstract

Section: Introductionmentioning

confidence: 99%

Zebrafish Vascular Mural Cell Biology: Recent Advances, Development, and Functions

Ando

Ishii

Fukuhara

2021

Life

View full text Add to dashboard Cite

show abstract

“…Similar to mammals, fibroblasts infiltration and prominent ECM deposition have been described after SCI in zebrafish [26,106], goldfish [107] and other regenerating vertebrates [8]. In zebrafish, a fibroblast population of perivascular and myoseptal origin that enters the lesion site can be identified by the expression of the platelet-derived growth factor receptor (Pdgfr) β, similar to what has been described in the mouse spinal cord [98,99].…”

Section: Fibroblastsmentioning

confidence: 59%

“…Thus, the initial cellular response to SCI appears to be largely conserved between zebrafish and non-regenerating vertebrates, although additional fibroblast subtypes likely exist in both species that contribute to the lesion site population [108]. Different to mammals however, in zebrafish, pdgfrb + fibroblasts are required for axon regeneration and functional recovery while cell autonomous signaling through the Pdgf receptor (Pdgfr) has been identified as a molecular cue controlling their recruitment [106] (Figures 2 and 3). Interestingly, Pdgf signaling also appears to be a conserved chemoattractant for fibroblasts contributing to organ regeneration in other species [109].…”

Section: Fibroblastsmentioning

confidence: 99%

Know How to Regrow—Axon Regeneration in the Zebrafish Spinal Cord

Tsata

Wehner

2021

Cells

Self Cite

View full text Add to dashboard Cite

The capacity for long-distance axon regeneration and functional recovery after spinal cord injury is poor in mammals but remarkable in some vertebrates, including fish and salamanders. The cellular and molecular basis of this interspecies difference is beginning to emerge. This includes the identification of target cells that react to the injury and the cues directing their pro-regenerative responses. Among existing models of successful spinal cord regeneration, the zebrafish is arguably the most understood at a mechanistic level to date. Here, we review the spinal cord injury paradigms used in zebrafish, and summarize the breadth of neuron-intrinsic and -extrinsic factors that have been identified to play pivotal roles in the ability of zebrafish to regenerate central nervous system axons and recover function.

show abstract

“…Zebrafish has also proven to be an excellent model because of its amazing regenerative potential to functionally heal a spinal cord injury (SCI). In collaboration with several groups in Germany, we have analysed how perivascular cells and the specific ECM components they secrete, promote regeneration zebrafish, while similar cells create a non-permissive environment that leads to scar formation in mammals following SCI (Tsata et al, 2021).…”

Section: Zebrafish Models Of Regenerationmentioning

confidence: 99%

Zebrafish research in Greece: swimming against the current

Beis

2022

Int. J. Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Zebrafish is a vertebrate model extensively used in Developmental Biology and Human Disease modeling as it shares high genetic and physiological similarities with humans. It has become the second most popular animal model, following mice, with several advantages: zebrafish are easily housed and cared for, the cost of installing and maintaining a zebrafish facility is significantly lower than mice, they reproduce often and develop quickly. Using zebrafish complies with the 3Rs principles of laboratory animal use. Zebrafish embryos develop externally and are transparent, allowing for in vivo non-invasive imaging. There are many transgenic and mutant lines available that mimic most Human Diseases, including reporter lines for most signaling pathways. There are also several reverse genetic tools to functionally verify genes or variants of unknown significance, identified in Genome-Wide Association Studies (GWAS) or using Next Generation Sequencing (NGS) approaches. In addition, the model emerges as an invaluable whole animal platform for various stages of drug discovery efforts by exploring the ability to do high-throughput phenotypic-driven screens. These include phenotypic screenings, determinations of general and/or specific toxicity (cardiac, renal, hepatotoxicity etc.), and mechanism of action studies. Finally, zebrafish are able to retain their capacity to regenerate most organs during their entire life span, making them a well-established model to study organ regeneration. The European Zebrafish Society consists of more than 180 research labs throughout Europe. In Greece however, zebrafish use remains rather limited. I present here a brief historical overview of zebrafish research in Greece.

show abstract

A switch in pdgfrb cell-derived ECM composition prevents inhibitory scarring and promotes axon regeneration in the zebrafish spinal cord

Cited by 59 publications

References 76 publications

Zebrafish Vascular Mural Cell Biology: Recent Advances, Development, and Functions

Zebrafish Vascular Mural Cell Biology: Recent Advances, Development, and Functions

Know How to Regrow—Axon Regeneration in the Zebrafish Spinal Cord

Zebrafish research in Greece: swimming against the current

Contact Info

Product

Resources

About