A switch phenomenon in the compound eye of the white-eyed mutant of Drosophila melanogaster

Cosens, Derek; Briscoe, David A.

doi:10.1016/0022-1910(72)90190-4

Cited by 65 publications

(22 citation statements)

References 7 publications

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“…aegypti. One sgRNA (W1-sgRNA) was designed to target exon 3 of the white gene (AAEL016999) (Supplementary Figure 4A) which is the Aedes 1:1 orthologue to D. melanogaster white and functions as an ATP-binding cassette transporter important for red and brown eye color pigmentation (57,58). Another sgRNA was designed to target exon 2 of the Yellow gene (AAEL006830) (Supplementary Figure 5A) which is the Aedes 1:1 orthologue to D. melanogaster yellow, a gene important for melanization of the cuticle (59,60).…”

Section: Germline Expression Of Cas9 In Ae Aegypti Increases Targetimentioning

confidence: 99%

Germline Cas9 Expression Yields Highly Efficient Genome Engineering in a Major Worldwide Disease Vector,Aedes aegypti

Bui

Yang

et al. 2017

Preprint

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The development of CRISPR/Cas9 technologies has dramatically increased the accessibility and efficiency of genome editing in many organisms. In general, in vivo germline expression of Cas9 results in substantially higher activity than embryonic injection. However, no transgenic lines expressing Cas9 have been developed for the major mosquito disease vector Aedes aegypti. Here, we describe the generation of multiple stable, transgenic Ae. aegypti strains expressing Cas9 in the germline, resulting in dramatic improvements in both the consistency and efficiency of genome modifications using CRISPR. Using these strains, we disrupted numerous genes important for normal morphological development, and even generated triple mutants from a single injection. We have also managed to increase the rates of homology directed repair by more than an order of magnitude. Given the exceptional mutagenic efficiency and specificity of

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Section: Germline Expression Of Cas9 In Ae Aegypti Increases Targetimentioning

confidence: 99%

Germline Cas9 Expression Yields Highly Efficient Genome Engineering in a Major Worldwide Disease Vector,Aedes aegypti

Bui

Yang

et al. 2017

Preprint

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“…This event is observed electrophysiologically as an extracellularly recorded prolonged corneal negative afterpotential (Cosens & Correspondence: Mr P. D. Morton Briscoe, 1972;Wright & Cosens, 1977), and an intracellularly recorded prolonged depolarizing after-potential (for retinula cells 1-6 of Drosophila, Minke et al, 1975; reported earlier in Limulus, Nolte et al, 1968, andin Balanus, Hochstein et al, 1973). In effect 'permanent' light channels are created through the receptor cell membranes allowing Na+ ions to enter the cell and depolarize the transmembrane potential.…”

Section: Introductionmentioning

confidence: 96%

Vision in Drosophila: evidence for the involvement of retinula cells 1–6 in the orientation behaviour of Drosophila melanogaster

Morton

Cosens

1978

Physiological Entomology

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ABSTRACT. Orientation responses of wildtype red‐eye, and mutant brown‐ and white‐eye Drosophila melanogaster were studied in a circular arena. Freely walking flies (‘closed‐loop’) were introduced into the arena and their orientations to a vertical black stripe of angular width 10> were recorded under different chromatic regimes. As has been reported previously, the accuracy of orientation and tropotactic responses were directly related to the extent of eye pigmentation. Orange‐adaptation was found to have little effect on the accuracy of orientation, but blue‐adaptation affected this orientation in all three phenotypes, with statistically significant diminutions in their responses. An electro‐physiological study confirmed the inferred effect of blue‐adaptation, namely that it causes a decreased sensitivity in retinula cells 1–6 by converting the visual pigment R480 to a stable metarhodopsin M580. It is concluded that these retinula cells are involved in orientation behaviour requiring a high degree of visual acuity in Drosophila.

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“…These mutants were isolated on the basis of their defect in the prolonged depolarizing afterpotential (PDA) produced by a bright blue stimulus that photoconverts a substantial net amount of rhodopsin to metarhodopsin ( Fig. 1) (5)(6)(7). In mutants with greatly reduced amounts of rhodopsin, the PDA is either very much reduced in size or totally absent (Fig.…”

mentioning

confidence: 99%

Gene encoding cytoskeletal proteins in Drosophila rhabdomeres.

Matsumoto¹,

Isono²,

Pye³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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The ninaC gene is one of eight nina (neither inactivation nor afterpotential) genes identified from mutations that drastically reduce the amount of rhodopsin in the compound eye of Drosophila melanogaster. The gene has been cytogenetically localized to the 27E-28B region of the second chromosome. NaDodSO4/PAGE analysis of eye proteins of flies carrying one, two, or three copies of the ninaC region shows that two eye-specific proteins of molecular weight 170,000 and 130,000 display a strong dependence on the dosage of the ninaC gene, although the dependence is evident only when the dosage is decreased and not when it is increased. All mutations in the ninaC gene studied to date have pronounced effects on these two polypeptides. These results suggest that the ninaC locus encodes these two polypeptides. Ultrastructural studies show that the polypeptides encoded by ninaC are very likely to be important components of the cytoskeletal structure of rhabdomeral microvilli.Among the many Drosophila melanogaster mutants that have been isolated in this laboratory on the basis of their defects in light-evoked electrical responses of the eye (1, 2) are those with greatly reduced amounts ofthe visual pigment, rhodopsin (2-4). These mutants were isolated on the basis of their defect in the prolonged depolarizing afterpotential (PDA) produced by a bright blue stimulus that photoconverts a substantial net amount of rhodopsin to metarhodopsin (Fig.

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A switch phenomenon in the compound eye of the white-eyed mutant of Drosophila melanogaster

Cited by 65 publications

References 7 publications

Germline Cas9 Expression Yields Highly Efficient Genome Engineering in a Major Worldwide Disease Vector,Aedes aegypti

Germline Cas9 Expression Yields Highly Efficient Genome Engineering in a Major Worldwide Disease Vector,Aedes aegypti

Vision in Drosophila: evidence for the involvement of retinula cells 1–6 in the orientation behaviour of Drosophila melanogaster

Gene encoding cytoskeletal proteins in Drosophila rhabdomeres.

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