“…The reference indicates that TNBS (2,4,6-trinitrobenzenesulphonate) is a TST enzyme inhibitor, which can effectively inhibit the production of SO 2 in HepG-2 cells. 31 Therefore, in the fourth column, HepG-2 cells were incubated in TNBS for 1 h, then transferred to CPO1 for 1 h, and finally incubated in a mixture of GSH and Na 2 S 2 O 3 for 1 h. After this, the fluorescence intensity was almost the same as that of the control group. In conclusion, probe CPO1 can be used for the detection of endogenous and exogenous SO 2 .…”