A synthetic nanofibrillar matrix promotes in vivo-like organization and morphogenesis for cells in culture

Schindler, Melvin; Ahmed, Ijaz; Kamal, Jabeen; Nur‐E‐Kamal, Alam; Grafe, Timothy H.; Chung, Heesung; Meiners, Scott J.

doi:10.1016/j.biomaterials.2005.02.014

Cited by 218 publications

(163 citation statements)

References 28 publications

Supporting

Mentioning

150

Contrasting

Unclassified

Order By: Relevance

“…We have created our nano ECM from an electrospun nonwoven matrix of polyamide nanofibers with an average fiber diameter of 280 nm [33], which was employed as a model to mimic the geometry and nanotopography of the ECM/basement membrane for cell growth [10]. The beneficial influence of this nanofibrillar surface has been reported in cultures of different somatic cells [1,2,25,27] and the differentiation of human embryonic stem cells into a hepatic lineage as well as mouse mesenchymal stem cells [13,31].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Behavior of mouse spermatogonial stem-like cells on an electrospun nanofibrillar matrix

Malek

Kohram

Shahverdi

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose Spermatogonial stem cells are affected by the interactions of extrinsic signals produced by components of the microenvironment niche, in addition to the chemical and physical properties of the extracellular matrix. Therefore, this study was initiated to assess the interaction of these cells on a synthetic nanofibrillar extracellular matrix that mimicked the geometry and nanotopography of the basement membrane for cellular growth. Methods This study has used a variety of experimental approaches to investigate the interaction of mouse neonatalderived spermatogonial stem-like cells on a synthetic random oriented three-dimensional nanofibrillar matrix composed of electrospun polyamide nanofibers (Ultra-Web™).Results Spermatogonial stem-like cell colonies were characterized by their ability to express α6-integrin, Thy-1, PLZF, and β1-integrin. After culture of cells on the nanofibrillar surfaces for 7 days, the number of colonies, the number of cells in each colony, and the average area of colonies were increased (P<0.05). However, the expression difference of related markers in both groups was not significant. A significantly higher proliferation and survival was observed in the nanofibrillar group (P<0.05). After transplantation into the testes of busulfan-treated adult mice, spermatogonial stem-like cell colonies that were cultured on the nanofibrillar surface demonstrated functionality, as verified by their ability to migrate to the seminiferous basal membrane, where they produced additional colonies. Conclusions These results have suggested that electrospun nanofibrillar surfaces could provide a more favorable microenvironment for in vitro short term culture of spermatogonial stem-like cell colonies.

show abstract

Section: Discussionmentioning

confidence: 99%

“…We evaluated their interactions (e.g., colony formation, proliferation, survival, stemness, and function) by a variety of experimental approaches. This synthetic nanofibrillar ECM mimicked the geometry and nanotopography of the basement membrane for cellular growth [27,33].…”

Section: Introductionmentioning

confidence: 99%

Behavior of mouse spermatogonial stem-like cells on an electrospun nanofibrillar matrix

Malek

Kohram

Shahverdi

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Scale bars: 50 µm. and organized assembly of different cell types in vitro the cells obtained by trypsinization of limbal tissue express the markers and characteristics of LSCs and that (2,5,18,25,35). The constructs formed by nanofiber scaffolds and specialized cells have been suggested as perthese cells can be propagated in vitro in tissue culture (13).…”

Section: Suppression Of a Local Inflammatory Reaction Nanofibers Contmentioning

confidence: 99%

Treatment of Ocular Surface Injuries by Limbal and Mesenchymal Stem Cells Growing on Nanofiber Scaffolds

et al. 2010

View full text Add to dashboard Cite

Stem cell (SC) therapy represents a promising approach to treat a wide variety of injuries, inherited diseases, or acquired SC deficiencies. One of the major problems associated with SC therapy remains the absence of a suitable matrix for SC growth and transfer. We describe here the growth and metabolic characteristics of mouse limbal stem cells (LSCs) and mesenchymal stem cells (MSCs) growing on 3D nanofiber scaffolds fabricated from polyamide 6/12 (PA6/12). The nanofibers were prepared by the original needleless electrospun Nanospider technology, which enables to create nanofibers of defined diameter, porosity, and a basis weight. Copolymer PA6/12 was selected on the basis of the stability of its nanofibers in aqueous solutions, its biocompatibility, and its superior properties as a matrix for the growth of LSCs, MSCs, and corneal epithelial and endothelial cell lines. The morphology, growth properties, and viability of cells grown on PA6/12 nanofibers were comparable with those grown on plastic. LSCs labeled with the fluorescent dye PKH26 and grown on PA6/12 nanofibers were transferred onto the damaged ocular surface, where their seeding and survival were monitored. Cotransfer of LSCs with MSCs, which have immunosuppressive properties, significantly inhibited local inflammatory reactions and supported the healing process. The results thus show that nanofibers prepared from copolymer PA6/12 represent a convenient scaffold for growth of LSCs and MSCs and transfer to treat SC deficiencies and various ocular surface injuries.

show abstract

“…Several cells types, including osteoblasts 78,79 , fibroblasts 80,81 , rat kidney cells 80 , smooth muscle cells 82 , neural stem cells 83 and embryonic stem cells 84 , have shown increased attachment on various nano-fibers compared to their corresponding control materials. Additionally, a recent study found that branched nano-fibers improve fibroblast attachment over linear nano-fibers 85 .…”

Section: Attachment and Proliferationmentioning

confidence: 99%

“…Several studies have noted differences in integrin expression on nano-fibrous materials compared to control 78,80,86,87 . Increased expression of α 2 , α V , β 1 , and β 3 integrins has been seen in osteoblasts on nano-fibrous scaffolds compared to comparable solid-walled scaffolds 78 .…”

Section: Attachment and Proliferationmentioning

confidence: 99%

Tissue engineering with nano-fibrous scaffolds

2008

View full text Add to dashboard Cite

Tissue Engineering is a rapidly evolving field in terms of cell source and scaffold fabrication. As the template for three dimensional tissue growth, the scaffold should emulate the native extracellular matrix, which is nano-fibrous. Currently, there are three basic techniques capable of generating nanofibrous scaffolding: electrospinning, molecular self-assembly, and thermally induced phase separation. These scaffolds can then be further modified by various three dimensional surface modification techniques if necessary to more precisely emulate the native extracellular matrix. However, even without further modification, nano-fibrous scaffolds have been shown to have advantageous effects on cellular behavior and tissue formation when compared to more traditional types of scaffolding. This review focuses on the current state of tissue engineering with nano-fibrous scaffolding with particular emphasis on bone tissue engineering.

show abstract

A synthetic nanofibrillar matrix promotes in vivo-like organization and morphogenesis for cells in culture

Cited by 218 publications

References 28 publications

Behavior of mouse spermatogonial stem-like cells on an electrospun nanofibrillar matrix

Behavior of mouse spermatogonial stem-like cells on an electrospun nanofibrillar matrix

Treatment of Ocular Surface Injuries by Limbal and Mesenchymal Stem Cells Growing on Nanofiber Scaffolds

Tissue engineering with nano-fibrous scaffolds

Contact Info

Product

Resources

About