“…Eyelid movement was measured by determining changes in infrared (IR) light reflection from the eye surface with dual IR emitter/ detector units located in front of each eye. 1,14,15 Animal experimentation adhered to protocols approved by the Massachusetts Eye and Ear Infirmary IRB and the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.Fifteen weeks after CN VII transection, 11 of these rats underwent a second surgical manipulation, in which resection of the ipsilateral RB muscle was performed via a superior orbitotomy in the retrobulbar region, transitioning them to an OO paralysis and RB myotomy state. Eye surgery was performed by a team of two microsurgeons, under microsurgical magnification (40ϫ) with a double-headed microscope (Wild M651; Leitz, Rockleigh, NJ), being careful not to damage the long ciliary nerves responsible for corneal sensation.…”