A systematic comparison of four bioaerosol generators: Affect on culturability and cell membrane integrity when aerosolizing Escherichia coli bacteria

Zhen, Huajun; Han, Taewon; Fennell, Donna E.; Mainelis, Gediminas

doi:10.1016/j.jaerosci.2014.01.002

Cited by 59 publications

(68 citation statements)

References 30 publications

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“…However, less than 10% collection efficiency was obtained for infectious IFV by the BioSampler. Possible reasons include: (a) loss of virus viability during the collection process, (b) poor physical collection attributed to enrichment of viable IFVs in the smallest particles, as "naked" IFVs can be as small as 80-120 nm (Zhen et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Highly efficient collection of infectious pandemic influenza H1N1 virus (2009) through laminar-flow water based condensation

Lednicky

Pan

Loeb

et al. 2016

Aerosol Science and Technology

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Section: Resultsmentioning

confidence: 99%

Highly efficient collection of infectious pandemic influenza H1N1 virus (2009) through laminar-flow water based condensation

Lednicky

Pan

Loeb

et al. 2016

Aerosol Science and Technology

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“…Even more surprising was the substantial increase in the bioluminescence output per bacterium when the polycarbonate jar was used. We speculate that mechanical stress during the aerosolization of P. fluorescens with both the glass and polycarbonate jars causes damage to bacterial membranes, including the release of intercellular material (Zhen et al, 2014); this partial lysis results in the release of intracellular ATP, which is then available to participate in bioluminescence reaction.…”

Section: Resultsmentioning

confidence: 99%

Application of ATP-based bioluminescence for bioaerosol quantification: Effect of sampling method

Han

Wren

DuBois

et al. 2015

Journal of Aerosol Science

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An adenosine triphosphate (ATP)-based bioluminescence has potential to offer a quick and affordable method for quantifying bioaerosol samples. Here we report on our investigation into how different bioaerosol aerosolization parameters and sampling methods affect bioluminescence output per bacterium, and implications of that effect for bioaerosol research. Bacillus atrophaeus and Pseudomonas fluorescens bacteria were aerosolized by using a Collison nebulizer (BGI Inc., Waltham, MA) with a glass or polycarbonate jar and then collected for 15 and 60 min with: (1) Button Aerosol Sampler (SKC Inc., Eighty Four, PA) with polycarbonate, PTFE, and cellulose nitrate filters, (2) BioSampler (SKC Inc.) with 5 and 20 mL of collection liquid, and (3) our newly developed Electrostatic Precipitator with Superhydrophobic Surface (EPSS). For all aerosolization and sampling parameters we compared the ATP bioluminescence output per bacterium relative to that before aerosolization and sampling. In addition, we also determined the ATP reagent storage and preparation conditions that that do not affect the bioluminescence signal intensity. Our results show that aerosolization by a Collison nebulizer with a polycarbonate jar yields higher bioluminescence output per bacterium compared to the glass jar. Interestingly enough, the bioluminescence output by P. fluorescens increased substantially after its aerosolization compared to the fresh liquid suspension. For both test microorganisms, the bioluminescence intensity per bacterium after sampling was significantly lower than that before sampling suggesting negative effect of sampling stress on bioluminescence output. The decrease in bioluminescence intensity was more pronounces for longer sampling times and significantly and substantially depended on the sampling method. Among the investigated method, the EPSS was the least injurious for both microorganisms and sampling times. While the ATP-based bioluminescence offers a quick bioaerosol sample analysis method, this works demonstrates that the method output depends on bioaerosol generation and sampling methods, as well as reagent storage.

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“…However, aerosolization can injure or fragment microorganisms [73]. We did not identify any study that quantifies the damage that aerosolization associated with showering or use of ultrasonic humidifiers causes to microorganisms.…”

Section: Application and Discussionmentioning

confidence: 99%

Assessing Inhalation Exposures Associated with Contamination Events in Water Distribution Systems

2016

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When a water distribution system (WDS) is contaminated, short-term inhalation exposures to airborne contaminants could occur as the result of domestic water use. The most important domestic sources of such exposures are likely to be showering and the use of aerosol-producing humidifiers, i.e., ultrasonic and impeller (cool-mist) units. A framework is presented for assessing the potential effects of short-term, system-wide inhalation exposures that could result from such activities during a contamination event. This framework utilizes available statistical models for showering frequency and duration, available exposure models for showering and humidifier use, and experimental results on both aerosol generation and the volatilization of chemicals during showering. New models for the times when showering occurs are developed using time-use data for the United States. Given a lack of similar models for how humidifiers are used, or the information needed to develop them, an analysis of the sensitivity of results to assumptions concerning humidifier use is presented. The framework is applied using network models for three actual WDSs. Simple models are developed for estimating upper bounds on the potential effects of system-wide inhalation exposures associated with showering and humidifier use. From a system-wide, population perspective, showering could result in significant inhalation doses of volatile chemical contaminants, and humidifier use could result in significant inhalation doses of microbial contaminants during a contamination event. From a system-wide perspective, showering is unlikely to be associated with significant doses of microbial contaminants. Given the potential importance of humidifiers as a source of airborne contaminants during a contamination event, an improved understanding of the nature of humidifier use is warranted.

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A systematic comparison of four bioaerosol generators: Affect on culturability and cell membrane integrity when aerosolizing Escherichia coli bacteria

Cited by 59 publications

References 30 publications

Highly efficient collection of infectious pandemic influenza H1N1 virus (2009) through laminar-flow water based condensation

Highly efficient collection of infectious pandemic influenza H1N1 virus (2009) through laminar-flow water based condensation

Application of ATP-based bioluminescence for bioaerosol quantification: Effect of sampling method

Assessing Inhalation Exposures Associated with Contamination Events in Water Distribution Systems

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