AimsThis study examined the anxiety levels of nurses and nurse candidates regarding humanoid nurse robots and artificial intelligence health technologies in perioperative patient care.DesignDescriptive and cross‐sectional study.MethodsThe research was conducted with 158 intern students and 167 surgical nurses. Socio‐demographic characteristics form, Questions Form Regarding Humanoid Nurse Robots and Artificial Intelligence Health Technologies, Artificial Intelligence Anxiety Scale and The Medical Artificial Intelligence Preparedness Scale were used. The independent t‐test and one‐way analysis of variance (ANOVA) were used. This study complied with Appendix S1.ResultsThe total scores on the Artificial Intelligence Anxiety Scale for nurses and nursing students are 73.089 ± 31.667 and 73.624 ± 28.029, respectively. The total scores on the Artificial Intelligence Readiness Scale for nurses and nursing students are 71.736 ± 15.064 and 72.183 ± 13.714, respectively. When comparing the sociodemographic characteristics and scale scores of nurses, a statistically significant difference was found between age and the Artificial Intelligence Anxiety Scale scores (p < 0.05). There was also a statistically significant difference between age, gender and work duration and the Artificial Intelligence Readiness Scale scores for nurses (p < 0.05).ConclusionBoth groups exhibited moderate levels of anxiety and readiness regarding artificial intelligence. Comprehensive research is needed to elucidate the impact of artificial intelligence technologies on nursing professionals.Implication for the ProfessionThe proper use of Artificial Intelligence technologies can enhance the quality of patient care, alleviate the workload, increase patient and staff satisfaction and foster new perspectives on acceptance. With their integration into clinics, a patient‐centred care environment will emerge, improving patient safety, outcomes and overall well‐being. Thus, the anxieties of nurses and students towards artificial intelligence technologies will decrease, and their readiness will increase.Patient or Public ContributionNo Patient or Public Contribution.