A Systematic Review of Extracellular Vesicle-Derived Piwi-Interacting RNA in Human Body Fluid and Its Role in Disease Progression

Goh, Tuan Xin; Tan, Sik Loo; Roebuck, Margaret M.; Teo, Seow Hui; Kamarul, Tunku

doi:10.1089/ten.tec.2022.0092

Cited by 11 publications

(13 citation statements)

References 56 publications

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“…The ncRNA signature also includes four piRNAs. In recent years, piRNAs have gained prominence as modulators of disease pathogenesis [ 42 ], and a recent systematic review analyzed the role of EV-derived piRNA in disease progression [ 13 ]. Only piR-20244 was associated with the GO terms mitochondrial inner membrane and cytochrome-c oxidase activity through the piRNAdb database.…”

Section: Discussionmentioning

confidence: 99%

“…Circulating ncRNAs can be detected in biofluids bound to RNA-binding proteins or packaged into extracellular vesicles (EVs), such as exosomes, functioning as paracrine effectors in the crosstalk between cell types [ 12 ]. Previous studies have established exosomal ncRNAs as biomarkers for diagnosis, prognosis and for monitoring therapy in immune diseases [ 9 , 13 , 14 , 15 ], and exosomal miRNA are the most studied in the SLE setting. Our group has identified exosomal miR-146a-3p levels associated with albuminuria, activity changes and disease flares in LN [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Plasma Exosomal Non-Coding RNA Profile Associated with Renal Damage Reveals Potential Therapeutic Targets in Lupus Nephritis

Flores-Chova

Martínez-Arroyo

Riffo‐Campos

et al. 2023

IJMS

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Despite considerable progress in our understanding of systemic lupus erythematosus (SLE) pathophysiology, patient diagnosis is often deficient and late, and this has an impact on disease progression. The aim of this study was to analyze non-coding RNA (ncRNA) packaged into exosomes by next-generation sequencing to assess the molecular profile associated with renal damage, one of the most serious complications of SLE, to identify new potential targets to improve disease diagnosis and management using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The plasma exosomes had a specific ncRNA profile associated with lupus nephritis (LN). The three ncRNA types with the highest number of differentially expressed transcripts were microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and piwi-interacting RNAs (piRNAs). We identified an exosomal 29-ncRNA molecular signature, of which 15 were associated only with LN presence; piRNAs were the most representative, followed by lncRNAs and miRNAs. The transcriptional regulatory network showed a significant role for four lncRNAs (LINC01015, LINC01986, AC087257.1 and AC022596.1) and two miRNAs (miR-16-5p and miR-101-3p) in network organization, targeting critical pathways implicated in inflammation, fibrosis, epithelial–mesenchymal transition and actin cytoskeleton. From these, a handful of potential targets, such as transforming growth factor-β (TGF-β) superfamily binding proteins (activin-A, TGFB receptors, etc.), WNT/β-catenin and fibroblast growth factors (FGFs) have been identified for use as therapeutic targets of renal damage in SLE.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plasma Exosomal Non-Coding RNA Profile Associated with Renal Damage Reveals Potential Therapeutic Targets in Lupus Nephritis

Flores-Chova

Martínez-Arroyo

Riffo‐Campos

et al. 2023

IJMS

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“…67 It is increasingly becoming obvious that piRNAs and PIWI proteins likely function at both the transcriptional and posttranscriptional stages, and they may even play a small but significant role in translation control. 68,69…”

Section: Piwi Proteins and Their Roles In Spermatogenesismentioning

confidence: 99%

“…Even though it has been shown that piRNAs and PIWI proteins are mostly expressed in the male germ cells of mammals and in the germ cells of many other animal species as well, research into their precise functions and associated processes is still in its infancy 67 . It is increasingly becoming obvious that piRNAs and PIWI proteins likely function at both the transcriptional and posttranscriptional stages, and they may even play a small but significant role in translation control 68,69 …”

Section: Pirna and Spermatogenesismentioning

confidence: 99%

Functions and mechanism of noncoding RNA in regulation and differentiation of male mammalian reproduction

Karoii

Azizi

2023

Cell Biochemistry & Function

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Noncoding RNAs (ncRNAs) are active regulators of a wide range of biological and physiological processes, including the majority of mammalian reproductive events. Knowledge of the biological activities of ncRNAs in the context of mammalian reproduction will allow for a more comprehensive and comparative understanding of male sterility and fertility. In this review, we describe recent advances in ncRNA‐mediated control of mammalian reproduction and emphasize the importance of ncRNAs in several aspects of mammalian reproduction, such as germ cell biogenesis and reproductive organ activity. Furthermore, we focus on gene expression regulatory feedback loops including hormones and ncRNA expression to better understand germ cell commitment and reproductive organ function. Finally, this study shows the role of ncRNAs in male reproductive failure and provides suggestions for further research.

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“…Due to increased access and delayed parenthood (Kocourkova et al, 2014;van Loendersloot et al, 2014), there is a steady rise in the use of ART including in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) (Sunderam et al, 2017). However, despite that ART has existed for several decades, the global overall success rate, as measured by live birth outcomes following a single embryo transfer, is approximately 30% (Smith et al, 2015;Gleicher et al, 2019;Khalife et al, 2020). This may prompt multiple attempts, constituting physical, financial and/or emotional burden among couples (Domar et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Non-coding RNAs from seminal plasma extracellular vesicles and success of live birth among couples undergoing fertility treatment

Oluwayiose

Houle

Whitcomb

et al. 2023

Front. Cell Dev. Biol.

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Background: Infertility remains a global health problem with male-factor infertility accounting for around 50% of cases. Understanding the molecular markers for the male contribution of live birth success has been limited. Here, we evaluated the expression levels of seminal plasma extracellular vesicle (spEV) non-coding RNAs (ncRNAs) in men of couples in relation with those with and without a successful live birth after infertility treatment.Method: Sperm-free spEV small RNA profiles were generated from 91 semen samples collected from male participants of couples undergoing assisted reproductive technology (ART) treatment. Couples were classified into two groups based on successful live birth (yes, n = 28) and (no, n = 63). Mapping of reads to human transcriptomes followed the order: miRNA > tRNA > piRNA > rRNA> “other” RNA > circRNA > lncRNA. Differential expression analysis of biotype-specific normalized read counts between groups were assessed using EdgeR (FDR<0.05).Result: We found a total of 12 differentially expressed spEV ncRNAs which included 10 circRNAs and two piRNAs between the live birth groups. Most (n = 8) of the identified circRNAs were downregulated in the no live birth group and targeted genes related to ontology terms such as negative reproductive system and head development, tissue morphogenesis, embryo development ending in birth or egg hatching, and vesicle-mediated transport. The differentially upregulated piRNAs overlapped with genomic regions including coding PID1 genes previously known to play a role in mitochondrion morphogenesis, signal transduction and cellular proliferation.Conclusion: This study identified novel ncRNAs profiles of spEVs differentiating men of couples with and without live birth and emphasizes the role of the male partner for ART success.

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A Systematic Review of Extracellular Vesicle-Derived Piwi-Interacting RNA in Human Body Fluid and Its Role in Disease Progression

Cited by 11 publications

References 56 publications

Plasma Exosomal Non-Coding RNA Profile Associated with Renal Damage Reveals Potential Therapeutic Targets in Lupus Nephritis

Plasma Exosomal Non-Coding RNA Profile Associated with Renal Damage Reveals Potential Therapeutic Targets in Lupus Nephritis

Functions and mechanism of noncoding RNA in regulation and differentiation of male mammalian reproduction

Non-coding RNAs from seminal plasma extracellular vesicles and success of live birth among couples undergoing fertility treatment

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