DC system is the main electrical equipment and control signal system in the substation, and its stability and reliability are directly related to whether the power system can operate safely. This paper firstly introduces the common methods of insulation detection of DC power system in substation and its advantages and disadvantages, and proposes the differential current method based on unbalanced bridge, i.e., using the combination of unbalanced bridge and leakage current sensor to detect the insulation condition of DC system, which makes up for the defects of the traditional differential current method that can only detect the faults of simple branch circuits, and realizes the detection of multi-branch grounding under complex situations. Finally, the feasibility of this method is verified by building a simulation model through simulink.