A systematic study of antibacterial silver nanoparticles: efficiency, enhanced permeability, and cytotoxic effects

Azócar, Manuel; Tamayo, Laura; Vejar, Nelson; Gómez, Grace; Zhou, Xiaorong; Thompsom, George; Cerda, Enrique; Kogan, Marcelo J.; Salas, Edison; Páez, Maritza

doi:10.1007/s11051-014-2465-4

Cited by 18 publications

(9 citation statements)

References 24 publications

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“…The percentage of bacteria in replication was lower with Ag-K than with the control, indicating that Ag-K interferes with the mechanisms of DNA replication or condensation [ 28 ]. This effect can be seen in the image on the right ( Figure 6 f), with condensed DNA strands that demonstrate antibacterial action in the cell [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

Stability of Antibacterial Silver Carboxylate Complexes against Staphylococcus epidermidis and Their Cytotoxic Effects

et al. 2018

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The antibacterial effects against Staphylococcus epidermidis of five silver carboxylate complexes with anti-inflammatory ligands were studied in order to analyze and compare them in terms of stability (in solution and after exposure to UV light), and their antibacterial and morphological differences. Four effects of the Ag-complexes were evidenced by transmission electronic microscopy (TEM) and scanning electronic microscopy (SEM): DNA condensation, membrane disruption, shedding of cytoplasmic material and silver compound microcrystal penetration of bacteria. 5-Chlorosalicylic acid (5Cl) and sodium 4-aminosalicylate (4A) were the most effective ligands for synthesizing silver complexes with high levels of antibacterial activity. However, Ag-5Cl was the most stable against exposure UV light (365 nm). Cytotoxic effects were tested against two kinds of eukaryotic cells: murine fibroblast cells (T10 1/2) and human epithelial ovarian cancer cells (A2780). The main objective was to identify changes in their antibacterial properties associated with potential decomposition and the implications for clinical applications.

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Section: Resultsmentioning

confidence: 99%

Stability of Antibacterial Silver Carboxylate Complexes against Staphylococcus epidermidis and Their Cytotoxic Effects

et al. 2018

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“…The nanosilver-based biomaterials and devices have found widespread applications in biomedicine, water and air purification, food production and preservation, cosmetics, clothing industry and numerous household products [20,21]. Several mechanisms have been reported for antibacterial activity of AgNPs including direct damage to the bacterial cell membrane, the release of silver ions and subsequent generation of reactive oxygen species which finally lead to the increased membrane permeability and DNA damage [20,22]. It has been reported that the size, shape and surface chemistry of AgNPs significantly affect their potential for cell penetration as well as their cytotoxicity and antibacterial activity.…”

Section: Antibacterial Activity Of Agnpsmentioning

confidence: 99%

Green synthesis of silver nanoparticles using Mentha pulegium and investigation of their antibacterial, antifungal and anticancer activity

Kelkawi

Kajani

Bordbar

2016

IET nanobiotechnol.

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A simple and eco-friendly method for efficient synthesis of stable colloidal silver nanoparticles (AgNPs) using extracts is described. A series of reactions was conducted using different types and concentrations of plant extract as well as metal ions to optimize the reaction conditions. AgNPs were characterized by using UV-vis spectroscopy, transmission electron microscopy, atomic force microscopy, dynamic light scattering, zetasizer, energy-dispersive X-ray spectroscopy (EDAX) and Fourier transform infrared spectroscopy (FTIR). At the optimized conditions, plate shaped AgNPs with zeta potential value of -15.7 and plasmon absorption maximum at 450 nm were obtained using high concentration of aqueous extract. Efficient adsorption of organic compounds on the nanoparticles was confirmed by FTIR and EDAX. The biogenic AgNPs displayed promising antibacterial activity on, , and. The highest antibacterial activity of 25 µg mL-1 was obtained for all the strains using aqueous extract synthesized AgNPs. The aqueous extract synthesised AgNPs also showed considerable antifungal activity against fluconazole resistant . The cytotoxicity assay revealed considerable anticancer activity of AgNPs on HeLa and MCF-7 cancer cells. Overall results indicated high potential of extract to synthesis high quality AgNPs for biomedical applications.

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“…It has been suggested that the cytotoxic and anti-bacterial effects of AgNPs are related to their physical and chemical features including sizes, shapes and coating agents. 39,40 The risk and the safe use of AgNPs for human health and environmental protection are still a controversial issue. Some previous studies claimed that AgNPs were harmless to cells 21 but other studies indicated they were cytotoxic.…”

Section: Physiochemical Properties Of Agnpsmentioning

confidence: 99%

Cytotoxic and sublethal effects of silver nanoparticles on tendon-derived stem cells – implications for tendon engineering

Cheung

Lau

et al. 2015

Toxicology Research

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Tendon injuries occur commonly in sports and workplace. Tendon-derived stem cells (TDSCs) have great potential for tendon healing because they can differentiate into functional tenocytes. To grow TDSCs properly , a scaffold is needed. Silver nanoparticles (AgNPs) have been used in a range of biomedical applications for their anti-bacterial and -inflammatory effects. AgNPs are therefore expected to be a good scaffolding coating material for tendon engineering. Yet, their cytotoxicity in TDSCs remains unknown. Moreover, their sublethal effects were mysterious in TDSCs. In our study, decahedral AgNPs (43.5 nm in diameter) coated with polyvinylpyrrolidone (PVP) caused a decrease in TDSCs' viability beginning at 37.5 μg ml but showed non-cytotoxic effects at concentrations below 18.8 μg ml. Apoptosis was observed in the TDSCs when higher doses of AgNPs (75-150 μg ml) were used. Mechanistically, AgNPs induced reactive oxygen species (ROS) formation and mitochondrial membrane potential (MMP) depolarization, resulting in apoptosis. Interestingly, treating TDSCs with -acetyl-l-cysteine (NAC) antioxidant significantly antagonized the ROS formation, MMP depolarization and apoptosis indicating that ROS accumulation was a prominent mediator in the AgNP-induced cytotoxicity. On the other hand, AgNPs inhibited the tendon markers' mRNA expression (0-15 μg ml), proliferation and clonogenicity (0-15 μg ml) in TDSCs under non-cytotoxic concentrations. Taken together, we have reported here for the first time that the decahedral AgNPs are cytotoxic to rat TDSCs and their sublethal effects are also detrimental to stem cells' proliferation and tenogenic differentiation. Therefore, AgNPs are not a good scaffolding coating material for tendon engineering.

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A systematic study of antibacterial silver nanoparticles: efficiency, enhanced permeability, and cytotoxic effects

Cited by 18 publications

References 24 publications

Stability of Antibacterial Silver Carboxylate Complexes against Staphylococcus epidermidis and Their Cytotoxic Effects

Stability of Antibacterial Silver Carboxylate Complexes against Staphylococcus epidermidis and Their Cytotoxic Effects

Green synthesis of silver nanoparticles using Mentha pulegium and investigation of their antibacterial, antifungal and anticancer activity

Cytotoxic and sublethal effects of silver nanoparticles on tendon-derived stem cells – implications for tendon engineering

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