A systematic study of high resolution multielemental quantitative bioimaging of animal tissue using LA-ICP-TOFMS

Abstract

Methodology for quantitative bioimaging of essential metals including Mg, Mn, Fe, Cu and Zn in tissue cryosections based on the use of high-resolution laser ablation coupled with inductively coupled plasma...

Atomic spectrometry update: review of advances in atomic spectrometry and related techniques

“…Between 10 and 15 ng Cr resulted in 3 53 Cr of ± 0.05 (2S). It was noted that samples measured by this total evaporation technique were found to have systematically lower 3 53 Cr and 3 54 Cr than were measured by the normal (non-total evaporation) TIMS method. It was suggested that this difference was caused by fractionation differences between the Cr metal and oxide species present during ionisation.…”

“…Methodology for the quantitative bioimaging of essential metals including Mg, Mn, Fe, Cu and Zn in tissue cryosections based on the use of high-resolution LA-ICP-TOFMS was developed by Strekopytov et al 54 Cryosectioned gelatin standards spiked with the elements of interest were used for calibration. Analysis under 'no gas' conditions showed satisfactory selectivity and 3-fold improvement in LODs for Mn, Fe and Cu when compared with those obtained using 2.4 mL min −1 H 2 in the collision/reaction cell of the ICP-TOFMS instrument.…”

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…The application of LA-ICP-TOF-MS for bioimaging has continued to demonstrate its ability as a powerful multi-elemental quasi-simultaneous technique. Strekopytov and co-workers 173 published a comprehensive study on the quantitative imaging of Cu Fe, Mg, Mn and Zn in animal tissue. A low-dispersion ablation cell was utilised alongside a dual concentric nebuliser for maximum sample transfer.…”

A Strategy for Quantitative Imaging of Lanthanide Tags in A549 Cells Using the Ratio of Internal Standard Elements

“…Gelatin calibration standards (10% w/w) were prepared as previously described. 33 Gelatin solutions were spiked with Eu and Ho solutions to achieve final calibration standard concentrations between 0 and 1000 mg kg −1 . A 200 μL aliquot of the spiked gelatin solutions was frozen directly inside a Leica CM1850 cryomicrotome (Leica Biosystems, Milton Keynes UK) at −17 °C, forming a rapidly freezing gelatin sphere from which 10 μm thick sections were cut onto SuperFrost microscope slides (Fisher Scientific, USA).…”