A Systematic Study of the Antibacterial Activity of Basidiomycota Crude Extracts

Clericuzio, Marco; Bivona, Mattia; Gamalero, Elisa; Bona, Elisa; Novello, Giorgia; Dovana, Francesco; Marengo, Emílio; Robotti, Elisa

doi:10.3390/antibiotics10111424

Cited by 12 publications

(9 citation statements)

References 37 publications

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“…Bacterial infections still represent a major health problem, amplified by the phenomenon of antibiotic resistance. Micromycetes have long been a reservoir of antibacterial drugs, but higher fungi, i.e., mushroom-forming fungi or macromycetes, are now emerging as a promising source of antimicrobial compounds [9,10].…”

Section: Discussionmentioning

confidence: 99%

Screening for Antibacterial Activity of French Mushrooms against Pathogenic and Multidrug Resistant Bacteria

et al. 2022

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In the alarming context of antibiotic resistance, we explored the antibacterial potential of French mushrooms against wild-type and multidrug-resistant (MDR) bacteria. In order to accelerate the discovery of promising compounds, screenings were carried out by TLC-direct bioautography. A total of 70 extracts from 31 mushroom species were evaluated against five wild-type bacteria: Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa. This first screening revealed that 95% of the extracts contained antibacterial compounds. Generally, it was observed that EtOAc extracts exhibited more active compounds than methanolic extracts. In addition, all extracts were overall more active against Gram-positive bacteria than against Gram-negative strains. The most promising mushroom extracts were then screened against various multidrug-resistant strains of S. aureus and E. coli. Activity was globally less on MDR strains; however, two mushroom species, Fomitopsis pinicola and Scleroderma citrinum, still contained several compounds inhibiting the growth of these MDR pathogenic bacteria. Stearic acid was identified as a ubiquitous compound contributing to the antibacterial defence of mushrooms. This screening revealed the potential of macromycetes as a source of antibacterial compounds; further assays are necessary to consider fungal compounds as promising drugs to counter antibiotic resistance.

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Section: Discussionmentioning

confidence: 99%

Screening for Antibacterial Activity of French Mushrooms against Pathogenic and Multidrug Resistant Bacteria

et al. 2022

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“…The metabolites produced by mushrooms and other fungi provide their extracts’ prebiotic and antimicrobial activities, as was observed in Clericuzio et al (2021) [ 35 ]. In this work, metabolites were obtained by extraction using 90% acetone and 10% 2-propanol ( v / v ) from the fruiting bodies of 16 species of Basidiomycetes.…”

Section: Discussionmentioning

confidence: 96%

Aqueous Extracts of Fermented Macrofungi Cultivated in Oilseed Cakes as a Carbon Source for Probiotic Bacteria and Potential Antibacterial Activity

et al. 2023

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Plant biomass colonized by macrofungi can contain molecules with bioactive properties with applications to human/animal health. This work aimed to verify antibacterial activities from aqueous extracts from oil seed cakes of Jatropha curcas (JSC) and cottonseed (CSC), fermented by macrofungi for probiotic bacteria cultivation. Coriolopsis sp., Tyromyces sp., Panus lecomtei, and Pleurotus pulmonarius were cultivated in solid and submerged media. The aqueous extract of unfermented JSC was more efficient than glucose for the growth of all probiotic bacteria. Extracts from four macrofungi fermented in CSC favored Lactobacillus acidophilus growth. In solid fermentation, macrofungi extracts cultivated in JSC favored Bifidobacterium lactis growth. All fungi extracts showed more significant growth than carbohydrates among the four probiotic bacteria evaluated. Regarding antimicrobial activities, no fungal extract or bacterial supernatant showed a more significant inhibition halo for enteropathogenic bacteria than ampicillin (control). Extracts from P. lecomtei and Coriolopsis sp. in CSC showed inhibition halos for Salmonella enterica. Supernatants from L. acidophilus, B. lactis, and Lactobacillus rhamnosus resulted in more significant inhibition of Staphylococcus aureus than the control, which indicates possible antimicrobial activity. Unfermented JSC supernatant showed better results for bacterial growth, while supernatants and aqueous extracts from CSC fermentation can be used for probiotic bacteria culture.

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“…Most of those pathogens are multidrug-resistant and are, therefore, a significant problem for global public health with enormous economic costs. Accordingly, there is an emerging need to find antimicrobials to combat ESKAPE pathogens and minimize their adverse effects [51]. Previously, Garcia et al [21] reported the importance of mushrooms as a source of valuable antibacterial compounds.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Antioxidant, Antibacterial, and Cosmeceutical Potential of Four Common Edible Mushrooms

et al. 2023

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Waste generated in mushroom cultivation represents a valuable raw material with health benefits, the use of which contributes to a reduction in food waste and to the circular economy. Hydroethanolic extracts obtained by ultrasound-assisted extraction of four edible mushroom species (Agaricus bisporus var. Portobello, Boletus edulis, Lentinula edodes, and Pleurotus ostreatus) were characterized according to their antioxidant, antibacterial, and cosmeceutical potential. The extract with the best properties was incorporated into a cosmetic cream. Concerning the phenolic contents and antioxidant capacity, the extracts of A. bisporus and B. edulis stood out from the other two due to their higher levels. The compounds p-hydroxybenzoic and gallic acids were present in all mushrooms in the ranges of 0.010 to 2.554 µg/g DW and 0.032 to 0.112 µg/g DW, respectively. The extract of B. edulis inhibited all the bacterial isolates, with minimum inhibitory concentration values ranging from 5 to 20 mg/mL for Gram-positive and 10–20 mg/mL for Gram-negative strains. In the anti-hyaluronidase assay, A. bisporus extract (10 mg/mL) was the most effective, with 74.4 ± 7.5% enzyme inhibition, and was selected for incorporation into the cosmetic cream. The cream with A. bisporus extract showed significantly higher phenolic contents and antioxidant capacity than the cream without the extract. Overall, our results support the high value of mushroom reuse as a source of natural antioxidants in the cosmetic industry.

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A Systematic Study of the Antibacterial Activity of Basidiomycota Crude Extracts

Cited by 12 publications

References 37 publications

Screening for Antibacterial Activity of French Mushrooms against Pathogenic and Multidrug Resistant Bacteria

Screening for Antibacterial Activity of French Mushrooms against Pathogenic and Multidrug Resistant Bacteria

Aqueous Extracts of Fermented Macrofungi Cultivated in Oilseed Cakes as a Carbon Source for Probiotic Bacteria and Potential Antibacterial Activity

Antioxidant, Antibacterial, and Cosmeceutical Potential of Four Common Edible Mushrooms

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